Photoautotroph-Methanotroph Coculture – A Flexible Platform for Efficient Biological CO2-CH4 Co-utilization

Badr, Kiumars; Hilliard, Matthew; Roberts, Nathan; He, Q. Peter; Wang, Jin

doi:10.1016/j.ifacol.2019.06.179

Cited by 15 publications

(10 citation statements)

References 9 publications

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“…Following the principles that drive the natural consortia, different synthetic methanotroph–photoautotroph (e.g., cyanobacteria or microalgae) cocultures have been demonstrated to simultaneously convert both CH 4 and CO 2 into microbial biomass without external oxygen supply (Badr et al, 2019; Hill et al, 2017; Rasouli et al, 2018; van der Ha et al, 2012). The biogas‐derived coculture biomass could be further processed to produce biofuels (such as biodiesel), directly used as single‐cell protein for animal feed supplement, or used as feedstock to produce bioplastics.…”

Section: Introductionmentioning

confidence: 99%

“…Figure 1a illustrates the key synergistic interactions within the methanotroph-photoautotroph coculture: the photoautotroph converts CO 2 into biomass while producing O 2 via photosynthesis and the methanotroph utilizes the in situ produced O 2 to convert CH 4 into biomass while producing CO 2 for the photoautotroph. Following the principles that drive the natural consortia, different synthetic methanotroph-photoautotroph (e.g., cyanobacteria or microalgae) cocultures have been demonstrated to simultaneously convert both CH 4 and CO 2 into microbial biomass without external oxygen supply (Badr et al, 2019;Hill et al, 2017;Rasouli et al, 2018;van der Ha et al, 2012). The biogas-derived coculture biomass could be further processed to produce biofuels (such as biodiesel), directly used as single-cell protein for animal feed supplement, or used as feedstock to produce bioplastics.…”

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confidence: 99%

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Fast and easy quantitative characterization of methanotroph–photoautotroph cocultures

Badr

Whelan

et al. 2020

Biotech & Bioengineering

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Recent research has demonstrated that synthetic methanotroph–photoautotroph cocultures offer a highly promising route to convert biogas into value‐added products. However, there is a lack of techniques for fast and accurate characterization of cocultures, such as determining the individual biomass concentration of each organism in real‐time. To address this unsolved challenge, we propose an experimental–computational protocol for fast, easy, and accurate quantitative characterization of the methanotroph–photoautotroph cocultures. Besides determining the individual biomass concentration of each organism in the coculture, the protocol can also obtain the individual consumption and production rates of O2 and CO2 for the methanotroph and photoautotroph, respectively. The accuracy and effectiveness of the proposed protocol was demonstrated using two model coculture pairs, Methylomicrobium alcaliphilum 20ZR–Synechococcus sp. PCC7002 that prefers high pH high salt condition, and Methylococcus capsulatus–Chlorella sorokiniana that prefers low salt and neutral pH medium. The performance of the proposed protocol was compared with a flow cytometry‐based cell counting approach. The experimental results show that the proposed protocol is much easier to carry out and delivers faster and more accurate results in measuring individual biomass concentration than the cell counting approach without requiring any special equipment.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Fast and easy quantitative characterization of methanotroph–photoautotroph cocultures

Badr

Whelan

et al. 2020

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following the principles that drive the natural consortia, different synthetic methanotroph-photoautotroph (e.g., cyanobacteria or microalgae) cocultures have been demonstrated to simultaneously convert both CH 4 and CO 2 into microbial biomass without external oxygen supply (Badr, Hilliard, Roberts, He, & Wang, 2019;Hill, Chrisler, Beliaev, & Bernstein, 2017;Rasouli, Valverde-Pérez, D'Este, De Francisci, & Angelidaki, 2018;van der Ha et al, 2012;Wang & He, 2018). The biogas-derived coculture biomass could be further processed to produce biofuels (such as biodiesel), directly used as single cell protein for animal feed supplement, or serves as feedstock to produce bioplastics.…”

Section: Introductionmentioning

confidence: 99%

Fast and Easy Quantitative Characterization of Methanotroph-Photoautotroph Cocultures

Badr

Whalen

et al. 2020

Preprint

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Recent research has demonstrated that synthetic methanotroph-photoautotroph cocultures offer a highly promising route to convert biogas into value-added products. However, there is a lack of techniques for fast and accurate characterization of cocultures, such as determining the individual biomass concentration of each organism in real-time. To address this unsolved challenge, we propose an experimental-computational protocol for fast, easy and accurate quantitative characterization of the methanotroph-photoautotroph cocultures. Besides determining the individual biomass concentration of each organism in the coculture, the protocol can also obtain the individual consumption and production rates of O2 and CO2 for the methanotroph and photoautotroph, respectively. The accuracy and effectiveness of the proposed protocol was demonstrated using two model coculture pairs, Methylomicrobium alcaliphilum 20ZR -Synechococcus sp. PCC7002 that prefers high pH high salt condition, and Methylococcus capsulatus -Chlorella sorokiniana that prefers low salt and neutral pH medium. The performance of the proposed protocol was compared with a flow cytometry based cell counting approach. The experimental results show that the proposed protocol is much easier to carry out and delivers faster and more accurate results in measuring individual biomass concentration than the cell counting approach without requiring any special equipment.

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“…buryatense[72], Methylocystis parvus[73], is worth noting that several methanotrophic genera have been reported to be capable of CO 2 fixation, which make them suitable candidates to grow on biogas; for instance,Methylococcus, Methylocaldum, and Methyloferula [utilize O 2 as an electron acceptor and H 2 as an electron donor, and fix CO 2 to biomass while assimilating ammonia nitrogen [78, 79]. According to the literatures, the following HOB genera (and species, if applicable) demonstrated to be capable of producing SCP.…”

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confidence: 99%

Conversion of biogas from anaerobic digestion to single cell protein and bio-methanol: mechanism, microorganisms and key factors - A review

Salehi¹,

Chaiprapat

2021

Environmental Engineering Research

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Biogas is a mixture of mainly CH 4 and CO 2 , which can be generated from anaerobic digestion of organic materials. The use of biogas is mostly limited to thermal energy and electricity generation. However, the abundance of cheap shale gas and solar energy could drive out biogas for such applications in the near future. Hence, alternative applications of biogas have recently received great attention among researchers across the globe. This review, for the first time, discusses the feasibility of the bioconversion of biogas to value-added products, including single cell protein as animal feed supplement, and methanol that is an important building block in the chemical industries. Mechanisms of the process, microorgnisms involved and key parameters affecting their efficiency are discussed in detail. Some future research needs are suggested in order to deepen our understanding of these biochemical processes.

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Photoautotroph-Methanotroph Coculture – A Flexible Platform for Efficient Biological CO2-CH4 Co-utilization

Cited by 15 publications

References 9 publications

Fast and easy quantitative characterization of methanotroph–photoautotroph cocultures

Fast and easy quantitative characterization of methanotroph–photoautotroph cocultures

Fast and Easy Quantitative Characterization of Methanotroph-Photoautotroph Cocultures

Conversion of biogas from anaerobic digestion to single cell protein and bio-methanol: mechanism, microorganisms and key factors - A review

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