Photoaffinity Labeling of the Guinea Pig Pulmonary Mast Cell Beta-adrenergic Receptor

Arbabian, Martin; Graziano, Filomena; Jicinsky, J; Hadcock, John R.; Malbon, Craig C.; Ruoho, Arnold E.

doi:10.1165/ajrcmb/1.5.351

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…This indicates that both m2 and m3 receptors are found in porcine and rat tracheal smooth muscle.48 Beta2 receptor mRNA has also been detected in human and rat lung49 and in guinea pig lung mast cells. 50 The amount of radioactivity in the labelled band can be quantified by laser densitometry to give a semiquantitative estimate of the amount of mRNA present. This makes it possible to assess changes in gene transcription of a particular receptor.…”

Section: Quantification and Localisation Of Receptor Transcription Nomentioning

confidence: 99%

Molecular biology and respiratory disease. 5. Molecular biology of receptors: implications for lung disease.

Barnes¹

1990

Thorax

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Receptors have a critical role in regulating lung function in health and disease.' Several such receptors have recently been sequenced, cloned, and genetically expressed. This opens up new vistas for investigating receptor regulation in pulmonary cells, for identifying receptor subtypes, and for understanding how disease may affect these regulatory processes. In the future it may also lead to new and more specific therapeutic approaches.This article attempts to discuss some of the areas in which molecular approaches have been used to study receptor structure and regulation. At present, these novel techniques have hardly been applied to pulmonary tissues and lung disease, but it is clear that these are extraordinarily powerful tools that may now address previously unanswerable questions. difficult to obtain a sufficient quantity of the receptor for the initial sequencing necessary to prepare a probe. More recently, the polymerase chain reaction has been used to amplify the number of mRNA copies in a cell in order to make a series of probes that can then be used to screen a DNA library.3The receptor DNA can also be inserted into an immortal cell line, resulting in its expression and thus providing a source of the receptor, so that the gene product can be characterised pharmacologically. The DNA may be microinjected directly into the large Xenopus (toad) oocyte, or transfected into a cultured mammalian cell that does not normally express the receptor by using a plasmid expression vector (ring of self replicating DNA into which the receptor DNA sequence of interest has been inserted), which after suitable manipulation inserts the DNA into the cultured cell genome. Cultured Chinese hamster ovary (CHO) and B-82 (a mouse L cell line) cells have been found to be very useful for studying neurotransmitter receptors because they lack these receptors but possess other elements such as G proteins and adenylyl cyclase, so that aspects of receptor coupling can be investigated. The cultured cells express the single receptor coded by the inserted DNA and this can be studied directly by radioligand binding and by measuring cellular responses, such as cyclic AMP concentrations or phosphoinositide hydrolysis. With the aid of a series of suitable agonists and antagonists the pure cloned receptor can then be characterised pharmacologically. Factors that influence the pure receptor can then be studied in detail without variations in metabolism and the interfering effects of related receptors.

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Section: Quantification and Localisation Of Receptor Transcription Nomentioning

confidence: 99%

Molecular biology and respiratory disease. 5. Molecular biology of receptors: implications for lung disease.

Barnes¹

1990

Thorax

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Bronchodilatatoren: Beta-2-Adrenergica

Soler¹

2000

Pharmakotherapie Bronchopulmonaler Erkrankungen

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Interactions of mast cells with the nervous system ?Recent advances

1992

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This article reviews recent advances in the understanding of mast cell-nervous system interactions. It is drawn largely from work published within the last ten years, and discusses the anatomical and biochemical evidence of a functional connection between mast cells and the nervous system, and the implications that such a relationship may have for normal and abnormal physiological functioning. Mast cells are found at varying levels of association with the nervous system; in CNS parenchyma (mainly thalamus), in connective tissue coverings (e.g. meninges, endoneurium), and in close apposition to peripheral nerve endings in a variety of tissues. There is, as yet, no clearly defined role for mast cells in nervous system function, or vice-versa, and it seems most likely that their interactions fulfil mutually modulatory roles. By extension, pathological situations where one of the partners in this relationship is overly stimulated may lead to a dysregulation of the other, and contribute to disease symptomatology.

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Photoaffinity Labeling of the Guinea Pig Pulmonary Mast Cell Beta-adrenergic Receptor

Cited by 4 publications

References 25 publications

Molecular biology and respiratory disease. 5. Molecular biology of receptors: implications for lung disease.

Molecular biology and respiratory disease. 5. Molecular biology of receptors: implications for lung disease.

Bronchodilatatoren: Beta-2-Adrenergica

Interactions of mast cells with the nervous system ?Recent advances

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