Photoadduct Leading to Crosslinking in Ru^II-Derivatized Oligonucleotides

Bioinorganic Photochemistry: Frontiers and Mechanisms

“…This strategy, so-called the anti-sense or anti-gene strategy, can be used to direct the photoreactive complex to a specific sequence. The photoactive ruthenium(II) complexes ([Ru(dip)(tap) 2 ] 2+ or [Ru(CH 3 CN)(dppz)(tpy)] 2+ ) have been attached to a oligonucleotide and used for irreversible cross linking of the two strands upon irradiation. ,,− For the former ruthenium complex it was found that electron transfer from the photoexcited complex to DNA initiates the formation of adducts between the metal complex and DNA (Figure ). Such behavior was also observed for other complexes such as [Ru(bpy) 3 - n (hat/tap) n ] 2+ ( n = 2, 3) , and [Ru(bpz) 3 ] 2+ .…”

Photo-oxidizing RuII complexes and light: Targeting biomolecules via photoadditions

“…Indeed, the formation of the new bond relies on the accessibility of the G residue to the photoreactive complex. In a very detailed study, the influence of the position of the G base in the strand sequence relative to the position of the complex (distance and orientation towards 3 and 5 ends) has been examined [127,128].…”

Detection of secondary structures in 17-mer Ru(ii)-labeled single-stranded oligonucleotides from luminescence lifetime studies

“…1). [6][7][8][9][10][11][12] These systems represent models for studying the photocrosslinking, which is triggered by the Ru(II) complex, between the probe-strand and target-strand. 13 Such Ru-ODN systems could be developed in the future either in the frame of the antisense strategy or as molecular tools in DNA diagnostics with however the problem related to a possible structuration as mentioned above.…”