Photoactivation of the cryptochrome/photolyase superfamily

Wang, Jing; Du, Xian-li; Pan, Wulue; Wang, Xiaojie; Wu, Wenjian

doi:10.1016/j.jphotochemrev.2014.12.001

Cited by 48 publications

(48 citation statements)

References 217 publications

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“…Such radical pairs exist only in the transient semiquinone form in the redox cycle of cryptochrome, formed either during photoreduction of the fully oxidized form by UV to blue light or during re-oxidation of the fully reduced form without the need of light (Solov'yov and Schulten, 2009;Nießner et al, 2013Nießner et al, , 2014Wiltschko et al, 2016; reviewed by Wang et al, 2015;Fig. 1B).…”

Section: Discussionmentioning

confidence: 99%

“…Shaded shapes correspond to radical pairs. Photoactivation of the light-absorbing cofactor of cryptochromes, flavin adenine dinucleotide (FAD), and possibly methenyltetrahydrofolic acid (MTHF), and the subsequent oxidation/reduction events have been extrapolated from studies on DNA photolyases (light-induced DNA repair proteins), which are closely related to cryptochromes (reviewed by Cashmore et al, 1999;Chaves et al, 2011;Wang et al, 2015). Left: after excitation by UV to blue light, the semiquinone radical (FADH inclination 69.8 deg) towards any of the four cardinal directions [magnetic North (mN) at geographic North (gN), South (gS), East (gE) or West (gW)], without changing magnetic inclination or total intensity (Muheim et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…Considering that cryptochromes are blue-light photosensitive proteins (Wang et al, 2015), the lack of orientation under 461 nm blue light was quite unexpected. Because we could not exclude that the failure to orient was a result of the relatively low light intensity in the maze, compared with the full-spectrum light used by Muheim et al (2016), we carried out a series of experiments under monochromatic light of higher intensity (15×10 16 -18×10 Table 2).…”

Section: High-intensity Light Experimentsmentioning

confidence: 99%

“…• ( peaks at about 495 and 580 nm); and a fully reduced state, FADH − , with no absorption in the visible part of the spectrum (Liu et al, 2010; reviewed by Wang et al, 2015). Magnetic compass orientation in diverse animal groups has been observed under both blue and green monochromatic light (reviewed by Muheim et al, 2002;Johnsen et al, 2007;Phillips et al, 2010a;Wiltschko et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…•− (Solov'yov and Schulten, 2009;Müller and Ahmad, 2011;Nießner et al, 2013Nießner et al, , 2014Wiltschko et al, 2016; reviewed by Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Zebra finches have a light-dependent magnetic compass similar to migratory birds

Pinzon-Rodriguez

Muheim

2017

Journal of Experimental Biology

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Birds have a light-dependent magnetic compass that provides information about the spatial alignment of the geomagnetic field. It is proposed to be located in the avian retina and mediated by a lightinduced, radical-pair mechanism involving cryptochromes as sensory receptor molecules. To investigate how the behavioural responses of birds under different light spectra match with cryptochromes as the primary magnetoreceptor, we examined the spectral properties of the magnetic compass in zebra finches. We trained birds to relocate a food reward in a spatial orientation task using magnetic compass cues. The birds were well oriented along the trained magnetic compass axis when trained and tested under low-irradiance 521 nm green light. In the presence of a 1.4 MHz radio-frequency electromagnetic (RF)-field, the birds were disoriented, which supports the involvement of radical-pair reactions in the primary magnetoreception process. Birds trained and tested under 638 nm red light showed a weak tendency to orient ∼45 deg clockwise of the trained magnetic direction. Under low-irradiance 460 nm blue light, they tended to orient along the trained magnetic compass axis, but were disoriented under higher irradiance light. Zebra finches trained and tested under high-irradiance 430 nm indigo light were well oriented along the trained magnetic compass axis, but disoriented in the presence of a RF-field. We conclude that magnetic compass responses of zebra finches are similar to those observed in nocturnally migrating birds and agree with cryptochromes as the primary magnetoreceptor, suggesting that light-dependent, radicalpair-mediated magnetoreception is a common property for all birds, including non-migratory species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: High-intensity Light Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…•− (Solov'yov and Schulten, 2009;Müller and Ahmad, 2011;Nießner et al, 2013Nießner et al, , 2014Wiltschko et al, 2016; reviewed by Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Zebra finches have a light-dependent magnetic compass similar to migratory birds

Pinzon-Rodriguez

Muheim

2017

Journal of Experimental Biology

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Photochemistry of Wild‐Type and N378D Mutant E. coli DNA Photolyase with Oxidized FAD Cofactor Studied by Transient Absorption Spectroscopy

et al. 2016

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DNA photolyases (PLs) and evolutionarily related cryptochrome (CRY) blue-light receptors form a widespread superfamily of flavoproteins involved in DNA photorepair and signaling functions. They share a flavin adenine dinucleotide (FAD) cofactor and an electron-transfer (ET) chain composed typically of three tryptophan residues that connect the flavin to the protein surface. Four redox states of FAD are relevant for the various functions of PLs and CRYs: fully reduced FADH(-) (required for DNA photorepair), fully oxidized FADox (blue-light-absorbing dark state of CRYs), and the two semireduced radical states FAD(.-) and FADH(.) formed in ET reactions. The PL of Escherichia coli (EcPL) has been studied for a long time and is often used as a reference system; however, EcPL containing FADox has so far not been investigated on all relevant timescales. Herein, a detailed transient absorption study of EcPL on timescales from nanoseconds to seconds after excitation of FADox is presented. Wild-type EcPL and its N378D mutant, in which the asparagine facing the N5 of the FAD isoalloxazine is replaced by aspartic acid, known to protonate FAD(.-) (formed by ET from the tryptophan chain) in plant CRYs in about 1.5 μs, are characterized. Surprisingly, the mutant protein does not show this protonation. Instead, FAD(.-) is converted in 3.3 μs into a state with spectral features that are different from both FADH(.) and FAD(.-) . Such a conversion does not occur in wild-type EcPL. The chemical nature and formation mechanism of the atypical FAD radical in N378D mutant EcPL are discussed.

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Response of Foodborne Pathogens to Ultraviolet Light

Yang

2022

Stress Responses of Foodborne Pathogens

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Photoactivation of the cryptochrome/photolyase superfamily

Cited by 48 publications

References 217 publications

Zebra finches have a light-dependent magnetic compass similar to migratory birds

Zebra finches have a light-dependent magnetic compass similar to migratory birds

Photochemistry of Wild‐Type and N378D Mutant E. coli DNA Photolyase with Oxidized FAD Cofactor Studied by Transient Absorption Spectroscopy

Response of Foodborne Pathogens to Ultraviolet Light

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