Photoactivated resveratrol against Staphylococcus aureus infection in mice

Santos, Denisar Palmito dos; Lopes, Diego Patrick Soares; Moraes, Ricardo Costa de; Gonçalves, Caroline Vieira; Rosa, Luciano Pereira; Rosa, Francine Cristina da Silva; Silva, Robson Amaro Augusto da

doi:10.1016/j.pdpdt.2019.01.005

Cited by 12 publications

(3 citation statements)

References 79 publications

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“…Despite this, there is no clear consensus on antiviral mechanism of action of resveratrol or emodin. Resveratrol and aloe-emodin, an isomer of emodin, were recently shown to be antimicrobial PSs [65][66][67]. These studies used blue LED photoactivation with comparable fluence to the white light LED source used herein; however, the concentrations for antimicrobial activity of photoactivated emodin (≥27 µg/mL) or resveratrol (2 mg/mL) were significantly higher than those in 4 µg/mL of PhytoQuin.…”

Section: Discussionmentioning

confidence: 94%

Photodynamic Inactivation of Human Coronaviruses

Duguay

Herod

Pringle

et al. 2022

Viruses

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Photodynamic inactivation (PDI) employs a photosensitizer, light, and oxygen to create a local burst of reactive oxygen species (ROS) that can inactivate microorganisms. The botanical extract PhytoQuinTM is a powerful photosensitizer with antimicrobial properties. We previously demonstrated that photoactivated PhytoQuin also has antiviral properties against herpes simplex viruses and adenoviruses in a dose-dependent manner across a broad range of sub-cytotoxic concentrations. Here, we report that human coronaviruses (HCoVs) are also susceptible to photodynamic inactivation. Photoactivated-PhytoQuin inhibited the replication of the alphacoronavirus HCoV-229E and the betacoronavirus HCoV-OC43 in cultured cells across a range of sub-cytotoxic doses. This antiviral effect was light-dependent, as we observed minimal antiviral effect of PhytoQuin in the absence of photoactivation. Using RNase protection assays, we observed that PDI disrupted HCoV particle integrity allowing for the digestion of viral RNA by exogenous ribonucleases. Using lentiviruses pseudotyped with the SARS-CoV-2 Spike (S) protein, we once again observed a strong, light-dependent antiviral effect of PhytoQuin, which prevented S-mediated entry into human cells. We also observed that PhytoQuin PDI altered S protein electrophoretic mobility. The PhytoQuin constituent emodin displayed equivalent light-dependent antiviral activity to PhytoQuin in matched-dose experiments, indicating that it plays a central role in PhytoQuin PDI against CoVs. Together, these findings demonstrate that HCoV lipid envelopes and proteins are damaged by PhytoQuin PDI and expands the list of susceptible viruses.

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Section: Discussionmentioning

confidence: 94%

Photodynamic Inactivation of Human Coronaviruses

Duguay

Herod

Pringle

et al. 2022

Viruses

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“…Furthermore, Kugaji et al demonstrated remarkable antibacterial and anti-biofilm activity of resveratrol against Porphyromonas gingivalis (P. gingivalis), a bacterium associated with gum disease (99). Dos Santos et al were the first to establish a connection between aPDT and resveratrol, highlighting its effective inhibition of S. aureus when used as a PS (61). Resveratrol as a natural polyphenol compound, exhibits therapeutic potential.…”

Section: Resveratrolmentioning

confidence: 99%

“…Dos Santos et al observed that blue LED light (54 J/cm 2 ) enhanced the antimicrobial effect of resveratrol (2 mg/mL, 100 µL) against MRSA. In a mouse abscess model, it induced the production of TNF-a and IL-17A cytokines, reduced bacterial burden, and consequently decreased inflammation 24 hours after infection (61). Ma et al demonstrated that AE-mediated aPDT effectively treated tinea corporis caused by T. rubrum in a guinea pig model and tinea unguium in an ex vivo model (67).…”

Section: In Vivo Apdt With Polyphenolsmentioning

confidence: 99%

Polyphenolic natural products as photosensitizers for antimicrobial photodynamic therapy: recent advances and future prospects

Wang,

Fekrazad

et al. 2023

Front. Immunol.

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Antimicrobial photodynamic therapy (aPDT) has become a potent contender in the fight against microbial infections, especially in the context of the rising antibiotic resistance crisis. Recently, there has been significant interest in polyphenolic natural products as potential photosensitizers (PSs) in aPDT, given their unique chemical structures and inherent antimicrobial properties. Polyphenolic natural products, abundant and readily obtainable from natural sources, are generally regarded as safe and highly compatible with the human body. This comprehensive review focuses on the latest developments and future implications of using natural polyphenols as PSs in aPDT. Paramount polyphenolic compounds, including curcumin, hypericin, quercetin, hypocrellin, celastrol, riboflavin, resveratrol, gallic acid, and aloe emodin, are elaborated upon with respect to their structural characteristics, absorption properties, and antimicrobial effects. Furthermore, the aPDT mechanism, specifically its targeted action on microbial cells and biofilms, is also discussed. Polyphenolic natural products demonstrate immense potential as PSs in aPDT, representing a promising alternate approach to counteract antibiotic-resistant bacteria and biofilm-related infections.

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Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

Xin

Kwek

et al. 2023

ChemPlusChem

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The emergence of multi‐drug resistant bacteria strains has been an uphill battle in modern healthcare worldwide, due to the increasing difficulty of killing them. The evolving pathogenicity of bacteria has led to researchers searching for more effective antimicrobial therapeutics to successfully eliminate them without undesirable consequences to the human body. In recent years, antimicrobial photodynamic therapy (APDT), an obsolete technique for cancer treatments, has been reported to eradicate bacteria and biofilm‐related infections. The principle of antimicrobial photodynamic therapy solely relies on the photosensitizers (PSs) generating reactive oxygen species, in the presence of oxygen and light, to destroy pathogens. Thus, it can target a broad spectrum of microorganisms, owing to the indirect interaction between PSs and the bacteria, resulting in the less likelihood for the development of drug resistant bacteria strains. This review will focus on the recent progress of APDT in the last five years and some future perspectives of APDT. The mechanism of APDT against bacteria and biofilms, various PSs used for APDT, and some common multidrug‐resistant bacteria strains will be briefly introduced. The reported in vivo applications of APDT in the several types of bacterial infections that includes periodontitis, wound infections, keratitis, endophthalmitis and tuberculosis in the last five years will be summarized in detail.

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Photoactivated resveratrol against Staphylococcus aureus infection in mice

Cited by 12 publications

References 79 publications

Photodynamic Inactivation of Human Coronaviruses

Photodynamic Inactivation of Human Coronaviruses

Polyphenolic natural products as photosensitizers for antimicrobial photodynamic therapy: recent advances and future prospects

Antimicrobial Photodynamic Therapy for the Remote Eradication of Bacteria

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