Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins

Zhou, Shengwang; Metcalf, Kevin J.; Bugga, Pradeep; Grant, Jennifer; Mrksich, Milan

doi:10.1021/acsami.8b16736

Cited by 12 publications

(7 citation statements)

References 59 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One important aspect of this solid-phase synthesis method is that it does not require the use of protecting groups and deprotection steps, ,,, which are necessary in the solid-phase synthesis of DNA, , peptides, , oligosaccharides, , and other molecules, − though we recognize there are a larger number of monomers used in those syntheses. This is because the covalent inhibitors on the linkers are poorly reactive with functional groups found on the surface of proteins and only react efficiently when they are present in the active site of the target enzyme.…”

mentioning

confidence: 99%

Solid-Phase Synthesis of Megamolecules

et al. 2020

Self Cite

View full text Add to dashboard Cite

This paper presents a solid-phase strategy to efficiently assemble multiprotein scaffoldsknown as megamolecules without the need for protecting groups and with precisely defined nanoscale architectures. The megamolecules are assembled through sequential reactions of linkers that present irreversible inhibitors for enzymes and fusion proteins containing the enzyme domains. Here, a fusion protein containing an N-terminal cutinase and a C-terminal SnapTag domain react with an ethyl pnitrophenyl phosphonate (pNPP) or a chloro-pyrimidine (CP) group, respectively, to give covalent products. By starting with resin beads that are functionalized with benzylguanine, a series of reactions lead to linear, branched, and dendritic structures that are released from the solid support by addition of TEV protease and that have sizes up to approximately 25 nm.

show abstract

mentioning

confidence: 99%

Solid-Phase Synthesis of Megamolecules

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…32,33 Regarding other technologies, as self-assembled monolayers (SAMs) of alkanethiols on coinage metals 34 or silanization at oxide surfaces, 35 many examples in the literature can be found where photolithography has been successfully used to pattern monolayer films. [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] Diverse photolabile functional groups have been described in the literature for this purpose as diazoketo 51 or p-methoxyphenacyl derivatives, 52 but o-nitrobenzyl derivatives [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] are by far the most used. Their synthesis is well-known and largely described in the literature.…”

Section: Introductionmentioning

confidence: 99%

Patterned organic layers on gold surfaces prepared by electro-grafting of photolabile-protected aryl diazonium salts

Taras,

Bergamini,

Brooksby

et al. 2024

RSC Appl. Interfaces

View full text Add to dashboard Cite

show abstract

“…The other major category is of parallel methods such as microcontact printing and mask-based photolithography, ,,− both of which allow fast replication but require the time-consuming and expensive production of a master stamp or mask and do not allow for the creation of complex gradients. Maskless photolithography, which uses computerized image projection systems, is quickly becoming a highly capable technique in the molecular printing field .…”

Section: Introductionmentioning

confidence: 99%

UV- and Visible-Light Photopatterning of Molecular Gradients Using the Thiol–yne Click Reaction

Mitmoen

Kedem

2022

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

The rational design of chemical coatings is used to control surface interactions with small molecules, biomolecules, nanoparticles, and liquids as well as optical and other properties. Specifically, micropatterned surface coatings have been used in a wide variety of applications, including biosensing, cell growth assays, multiplexed biomolecule interaction arrays, and responsive surfaces. Here, a maskless photopatterning process is studied, using the photocatalyzed thiol−yne "click" reaction to create both binary and gradient patterns on thiolated surfaces. Nearly defect-free patterns are produced by first coating glass surfaces with mercaptopropylsilatrane, a silanizing agent that forms smoother self-assembled monolayers than the commonly used 3-mercaptopropyltrimethoxysilane. Photopatterning is then performed using UV (365 nm) or visible (405 nm) light to graft molecules onto the surface in tunable concentrations based on the local exposure. The technique is demonstrated for multiple types of molecular grafts, including fluorescent dyes, poly(ethylene glycol), and biotin, the latter allowing subsequent deposition of biomolecules via biotin− avidin binding. Patterning is demonstrated in water and dimethylformamide, and the process is repeated to combine molecules soluble in different phases. The combination of arbitrary gradient formation, broad applicability, a low defect rate, and fast prototyping thanks to the maskless nature of the process creates a particularly powerful technique for molecular surface patterning that could be used for a wide variety of micropatterned applications.

show abstract

Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins

Cited by 12 publications

References 59 publications

Solid-Phase Synthesis of Megamolecules

Solid-Phase Synthesis of Megamolecules

Patterned organic layers on gold surfaces prepared by electro-grafting of photolabile-protected aryl diazonium salts

UV- and Visible-Light Photopatterning of Molecular Gradients Using the Thiol–yne Click Reaction

Contact Info

Product

Resources

About