Photoacoustic spectroscopy to evaluate the penetration of two antifungal agents through the human nail

Nuglisch, L.E.R.; Dias, Daniele Toniolo; Sehn, Elizandra; Bento, A. C.; Baesso, Mauro Luciano; Santos, S.T.S.; Fushimi, M.Y.

doi:10.1051/jp4:2005125144

Cited by 8 publications

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“…Finally, by means of the determination of the thermal diffusivity, the thermal diffusion length of the human nail was determined to be of the order 38 μm. These results were used in the spectroscopic evaluation of antifungal agent’s penetration into human fingernails (24), which constitute a very important study for the development of new therapeutic formulations.…”

Section: Resultsmentioning

confidence: 99%

Thermal Characterization In Vitro of Human Nail: Photoacoustic Study of the Aging Process

Dias

Steimacher

Bento

et al. 2007

Photochem & Photobiology

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In the present work, the rear photoacoustic signal technique is used to determine thermal properties of human nails. The aging process of the human nail is analyzed through its thermal diffusivity and specific heat and using these results, thermal conductivity and thermal effusivity is determined. The study in vitro of this natural polymer showed a minimum for thermal properties to age about 20 years and an increase and possible saturation of them for ages over 50 years. The minimum value found for thermal diffusivity was close to 10 · 10 )4 cm 2 s )1 with saturation near 18 · 10 )4 cm 2 s )1 . Thermal conductivity and effusivity presents the same behavior.

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Section: Resultsmentioning

confidence: 99%

Thermal Characterization In Vitro of Human Nail: Photoacoustic Study of the Aging Process

Dias

Steimacher

Bento

et al. 2007

Photochem & Photobiology

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“…The therapeutic effectiveness of medicines applied topically is related, among other factors, to their diffusion rate ( Sugiura et al, 2014 ). PAS has been applied successfully in the evaluation of the penetration of medicines in non-homogeneous and opaque samples, such as the skin ( Ames et al, 2017 ; Borghi-Pangoni et al, 2017 ), tooth ( Occhi-Alexandre et al, 2018 ), and nail ( Nuglisch et al, 2005 ). In the present study, PAS efficiently showed that PE was able to reach the deep layers of the nail by itself, which illustrates an additional property of this natural compound.…”

Section: Discussionmentioning

confidence: 99%

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

et al. 2018

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Onychomycosis is a chronic fungal infection of nails, commonly caused by dermatophyte fungi, primarily species of Trichophyton. Because of the limited drug arsenal available to treat general fungal infections and the frequent failure of onychomycosis treatment, the search for new therapeutic sources is essential, and topical treatment with natural products for onychomycosis has been encouraged. Propolis, an adhesive resinous compound produced by honeybees (Apis mellifera), has shown multiple biological properties including significant antifungal and anti-biofilm activities in vitro. In spite of promising in vitro results, in vivo results have not been reported so far. This study assessed an ethanol propolis extract (PE) as a topical therapeutic option for onychomycosis, including its characterization in vitro and its applicability as a treatment for onychomycosis (from bench to clinic). The in vitro evaluation included analysis of the cytotoxicity and the antifungal activity against the planktonic cells and biofilm formed by Trichophyton spp. We also evaluated the capacity of PE to penetrate human nails. Patients with onychomycosis received topical PE treatments, with a 6-month follow-up period. The results of the in vitro assays showed that PE was non-toxic to the cell lines tested, and efficient against both the planktonic cells and the biofilm formed by Trichophyton spp. The results also showed that PE is able to penetrate the human nail. The results for PE applied topically to treat onychomycosis were promising, with complete mycological and clinical cure of onychomycosis in 56.25% of the patients. PE is an inexpensive commercially available option, easy to obtain and monitor. Our results indicated that PE is a promising natural compound for onychomycosis treatment, due to its ability to penetrate the nail without cytotoxicity, and its good antifungal performance against species such as Trichophyton spp. that are resistant to conventional antifungals, both in vitro and in patients.

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“…[8][9][10] Therapy with topical application of fluoride or new drugs can be monitored since the thermal diffusivity is known. 11 The Open Photoacoustic Cell Technique (OPC) 12-14 is a non-destructive and low-cost technique used to find the thermal diffusivity in vitro with minimal amount of sample, specially dental materials. [15][16][17] In this context, the OPC technique became apt to find the effective thermal diffusivity of human dental samples.…”

Section: Introductionmentioning

confidence: 99%

In vitro thermal diffusivity measurements as aging process study in human tooth hard tissues

Carvalho¹,

Dias²,

Bedeschi³

et al. 2013

Journal of Applied Physics

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Photoacoustic spectroscopy to evaluate the penetration of two antifungal agents through the human nail

Cited by 8 publications

References 0 publications

Thermal Characterization In Vitro of Human Nail: Photoacoustic Study of the Aging Process

Thermal Characterization In Vitro of Human Nail: Photoacoustic Study of the Aging Process

Propolis Extract for Onychomycosis Topical Treatment: From Bench to Clinic

In vitro thermal diffusivity measurements as aging process study in human tooth hard tissues

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