Photoacoustic microscopy of blood pulse wave

Yeh, Cheng-Hung; Hu, Song; Maslov, Konstantin; Wang, Lihong V.

doi:10.1117/1.jbo.17.7.070504

Cited by 36 publications

(33 citation statements)

References 12 publications

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“…With high sensitivity for vasculature imaging thanks to the strong optical absorption contrast provided by hemoglobin in red blood cells, PAT has proven capable of providing structural, functional, and mechanical properties of blood vessels in both animal models and humans [16][17][18][19] . PAT has also been applied to measure the elastic properties of biological tissue.…”

Section: Introductionmentioning

confidence: 99%

Vascular elastic photoacoustic tomography in humans

et al. 2016

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Quantification of vascular elasticity can help detect thrombosis and prevent life-threatening conditions such as acute myocardial infarction or stroke. Here, we propose vascular elastic photoacoustic tomography (VE-PAT) to measure vascular elasticity in humans. VE-PAT was developed by incorporating a linear-array-based photoacoustic computed tomography system with a customized compression stage. By measuring the deformation of blood vessels under uniaxial loading, VE-PAT was able to quantify the vascular compliance. We first demonstrated the feasibility of VE-PAT in blood vessel phantoms. In large vessel phantoms, VE-PAT detected a decrease in vascular compliance due to simulated thrombosis, which was validated by a standard compression test. In small blood vessel phantoms embedded 3 mm deep in gelatin, VE-PAT detected elasticity changes at depths that are difficult to image using other elasticity imaging techniques. We then applied VE-PAT to assess vascular compliance in a human subject and detected a decrease in vascular compliance when an occlusion occurred downstream from the measurement point, demonstrating the potential of VE-PAT in clinical applications such as detection of deep venous thrombosis.

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Section: Introductionmentioning

confidence: 99%

Vascular elastic photoacoustic tomography in humans

et al. 2016

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“…OR-PAM realizes the key benefits of PAT in biological experimentation through its high-resolution imaging capability; further, its unique optical absorption-based contrast mechanism enables it to complement conventional highresolution microscopy tools, such as confocal microscopy [44][45][46][47][48][49], two-photon microscopy [44][45][46][50][51][52][53], and optical coherence tomography [44][45][46][53][54][55][56]. So far, considerable efforts have been made to improve the spatial resolution [15], scanning speed [7,12], and functional imaging capability [4][5][6][7][8][9] of OR-PAM. However, OR-PAM has not yet been intensively used for intravital microscopy (IVM) [44][45][46]; most previous studies imaged the body surfaces of small animals or in vitro specimens.…”

Section: Introductionmentioning

confidence: 99%

“…Since Maslov et al [1] reported the first experimental demonstration of optical-resolution photoacoustic microscopy (OR-PAM) in 2008, the technique has become a major experimental tool [2][3][4][5][6][7][8][9][10][11][12][13][14][15] of photoacoustic tomography (PAT) . OR-PAM realizes the key benefits of PAT in biological experimentation through its high-resolution imaging capability; further, its unique optical absorption-based contrast mechanism enables it to complement conventional highresolution microscopy tools, such as confocal microscopy [44][45][46][47][48][49], two-photon microscopy [44][45][46][50][51][52][53], and optical coherence tomography [44][45][46][53][54][55][56].…”

Section: Introductionmentioning

confidence: 99%

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang

Chen

et al. 2015

SPIE Proceedings

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Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an opticalresolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ~10 μm at an optical working distance of 6.5 mm. The OR-PAEM system's high-resolution intravital imaging capability is demonstrated through animal experiments.

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“…Optical resolution photoacoustic microscopy (OR-PAM), which is one version of PAT, uses endogenous hemoglobin absorption contrast to image the microvasculature [9][10][11]. The detailed images provided by OR-PAM pose interesting challenges in interpretation and classification.…”

Section: Introductionmentioning

confidence: 99%

Early-stage tumor detection using photoacoustic microscopy: a pattern recognition approach

Yeh

Wang

Liang

et al. 2017

Photons Plus Ultrasound: Imaging and Sensing 2017

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We report photoacoustic microscopy (PAM) of arteriovenous (AV) shunts in early stage tumors in vivo, and develop a pattern recognition framework for computerized tumor detection. Here, using a high-resolution photoacoustic microscope, we implement a new blood oxygenation (sO 2 )-based disease marker induced by the AV shunt effect in tumor angiogenesis. We discovered a striking biological phenomenon: There can be two dramatically different sO 2 values in bloodstreams flowing side-by-side in a single vessel. By tracing abnormal sO 2 values in the blood vessels, we can identify a tumor region at an early stage. To further automate tumor detection based on our findings, we adopt widely used pattern recognition methods and develop an efficient computerized classification framework. The test result shows over 80% averaged detection accuracy with false positive contributing 18.52% of error test samples on a 50 PAM image dataset.

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Photoacoustic microscopy of blood pulse wave

Cited by 36 publications

References 12 publications

Vascular elastic photoacoustic tomography in humans

Vascular elastic photoacoustic tomography in humans

Label-free optical-resolution photoacoustic endomicroscopy in vivo

Early-stage tumor detection using photoacoustic microscopy: a pattern recognition approach

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