Photoacoustic imaging of fear conditioning neuronal ensembles using a Fos-LacZ gene reporter system in the rat brain in vivo after intrathecal X-gal injection

Matchynski, James I.; Manwar, Rayyan; Kratkiewicz, Karl; Madangopal, Rajtarun; Lennon, Veronica A; Makki, Kassem M.; Hope, Bruce T.; Conti, Alana C.; Perrine, Shane A.; Avanaki, Kamran

doi:10.1117/12.2579526

Cited by 1 publication

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“…[26][27][28] Many neuroscience questions can be answered by a high-resolution functional brain imaging system. 22,29,30 For functional imaging, PAI relies on the same principle as that of functional magnetic resonance imaging, where neural activity is indirectly captured through imaging of cerebral hemodynamic fluctuations (i.e., changes in oxygen demand). Many applications of PAI for functional brain imaging have been reported.…”

Section: Introductionmentioning

confidence: 99%

“…PAI has previously been used to provide high-resolution structural and functional images of brain vasculature in small and large animal models 26 – 28 Many neuroscience questions can be answered by a high-resolution functional brain imaging system 22 , 29 , 30 . For functional imaging, PAI relies on the same principle as that of functional magnetic resonance imaging, where neural activity is indirectly captured through imaging of cerebral hemodynamic fluctuations (i.e., changes in oxygen demand).…”

Section: Introductionmentioning

confidence: 99%

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Spiral laser scanning photoacoustic microscopy for functional brain imaging in rats

Zafar,

McGuire,

Ranjbaran

et al. 2024

Neurophoton.

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There are many neuroscience questions that can be answered by a high-resolution functional brain imaging system. Such a system would require the capability to visualize vasculature and measure neural activity by imaging the entire brain continually and in rapid succession in order to capture hemodynamic changes. Utilizing optical excitation and acoustic detection, photoacoustic technology enables label-free quantification of changes in endogenous chromophores, such as oxyhemoglobin, deoxyhemoglobin, and total hemoglobin. Aim: Our aim was to develop a sufficiently high-resolution, fast frame-rate, and wide field-of-view (FOV) photoacoustic microscopy (PAM) system for the purpose of imaging vasculature and hemodynamics in a rat brain.Approach: Although the most PA microscopy systems use raster scanning (or less commonly Lissajous scanning), we have developed a simple-to-implement laser scanning optical resolution PAM system with spiral scanning (which we have named "spiral laser scanning photoacoustic microscopy" or sLS-PAM) to acquire an 18 mm diameter image at fast frame rate (more than 1 fps). Such a system is designed to permit continuous rat brain imaging without the introduction of photobleaching artifacts. Conclusion:We demonstrated the functional imaging capability of the sLS-PAM system by imaging cerebral hemodynamics in response to whisker and electrical stimulation and used it for vascular imaging of a modeled brain injury. We believe that we have demonstrated the development of a simple-to-implement PAM system, which could become an affordable functional neuroimaging tool for researchers.

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