Photoacoustic imaging of early inflammatory response using gold nanorods

Kim, Kang; Huang, Sheng Wen; Ashkenazi, Shai; O’Donnell, Matthew; Agarwal, Ashish; Kotov, Nicholas A.; Denny, Michael F.; Kaplan, Mariana J.

doi:10.1063/1.2743752

Cited by 157 publications

(126 citation statements)

References 21 publications

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“…S3). It has been demon- strated that a variety of gold nanocolloids are already entering in vivo clinical trials (19,(29)(30)(31)(32)(33). Among them, Au nanorods present particularly good optical absorption in the near-infrared region, tunable by changing the aspect ratio.…”

Section: B and Dmentioning

confidence: 99%

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

Bouchard

Anwar

Liu

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

170

126

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Multimodality imaging based on complementary detection principles has broad clinical applications and promises to improve the accuracy of medical diagnosis. This means that a tracer particle advantageously incorporates multiple functionalities into a single delivery vehicle. In the present work, we explore a unique combination of MRI and photoacoustic tomography (PAT) to detect picomolar concentrations of nanoparticles. The nanoconstruct consists of ferromagnetic (Co) particles coated with gold (Au) for biocompatibility and a unique shape that enables optical absorption over a broad range of frequencies. The end result is a dual-modality probe useful for the detection of trace amounts of nanoparticles in biological tissues, in which MRI provides volume detection, whereas PAT performs edge detection.dual-modality imaging ͉ ferromagnetic nanoparticle ͉ molecular imaging ͉ MRI contrast ͉ photoacoustic tomography W e have synthesized nanoparticles for dual-modality (1-7)MRI and photoacoustic tomography (PAT). The incorporation of MRI and PAT into a single probe offers the unique possibility of combining the complementary strategies of contrastbased volume imaging and edge detection. Our nanoconstruct consists of zero-valence ferromagnetic cobalt (Co) particles (8) with a gold (Au) coating for biocompatibility and a unique shape rendering increased optical absorption over a broad range of frequencies (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). This research theme follows the rapid developments in nanotechnology, diagnostic radiology, and targeted molecular imaging (20), whereby nanoparticulate contrast agents, with the desirable properties of high chemical specificity, biocompatibility, and a reasonable half-life, are administered within a specific region of interest. In nanoparticle-based imaging studies, higher particle concentrations lead to better signal-to-noise contrasts, but this also poses a tradeoff with the toxicity. Therefore, one of the most important parameters when developing particle-based contrast is the safest and lowest nanoparticle concentration that offers sufficient contrast sensitivity.In MRI, magnetic materials such as gadolinium chelates and magnetic nanoparticles are often used (21-23) to enhance image contrast. The magnetic nanoparticles are passivated by biocompatible coatings such as dextrin, citrate, polystyrene/divinylbenzene, and elemental gold. These coatings also detoxify the particles, resulting in enhanced lifetimes in vivo. Typical examples of magnetic nanoparticulate core-shell configurations include magnetitedextrin, magnetite-silica (24) and iron-gold (25).Laser-based PAT (9-19) is a hybrid imaging modality [see supporting information (SI) Fig. S1]. It uses a pulsed laser source to illuminate a biological sample. Light absorption by the tissue results in a transient temperature rise on the order of 10 mK. The rapid thermoelastic expansion excites ultrasonic waves that are measured by using broadband ultrasonic transducers conformally arranged around the sample. Finally, a modif...

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Section: B and Dmentioning

confidence: 99%

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

Bouchard

Anwar

Liu

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

170

126

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“…11 Absorption of light by GNRs is followed by predominantly nonradiative de-excitation processes and the released heat and subsequent temperature rise have applications in improving photoacoustic signals. 12,13 Further the temperature rise can be made sufficiently high to cause cell death by hyperthermia, which has potential for important therapeutic applications. [14][15][16] In the last mentioned application, the ability to redshift LP peaks by tailoring the dimensions of the GNRs coupled with the fact that biological tissue is relatively transparent in the red and NIR wavelengths makes GNRs very attractive.…”

Section: Introductionmentioning

confidence: 99%

Discrete dipole approximation simulations of gold nanorod optical properties: Choice of input parameters and comparison with experiment

Ungureanu

Rayavarapu

Manohar

et al. 2009

Journal of Applied Physics

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Gold nanorods have interesting optical properties due to surface plasmon resonance effects. A variety of biomedical applications of these particles have been envisaged and feasibilities demonstrated in imaging, sensing, and therapy based on the interactions of light with these particles. In order to correctly interpret experimental data and tailor the nanorods and their environments for optimal use in these applications, simulations of the optical properties of the particles under various conditions are essential. Of various numerical methods available, the discrete dipole approximation (DDA) approach implemented in the publicly available DDSCAT code is a powerful method that had proved popular for studying gold nanorods. However, there is as yet no universal agreement on the shape used to represent the nanorods and on the dielectric function of gold required for the simulations. We systematically study the influence of these parameters on simulated results. We find large variations in the position of plasmon resonance peaks, their amplitudes, and shapes of the spectra depending on the choice of the parameters. We discuss these in the light of experimental optical extinction spectra of gold nanorods synthesized in our laboratory. We show that much care should be taken and prudence applied before DDA results be used to interpret experimental data and to help characterize nanoparticles synthesized.

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“…Excess PAA in solution was removed by brief centrifugation and GNRs were dispersed in 10 ml of PBS followed by the addition of 1.25 ml of 1M N-ethyl-N-3-dimethylamino propyl-carbodiimide (EDC) (Sigma-Aldrich, St. Louis, MO, USA) and 1.25 ml of 1M N-hydroxy-succinimide (NHS) (Sigma-Aldrich, St. Louis, MO, USA). [10][11][12][13][14]16] After 20 min, the reaction mixture was added to 20 µl of FITC labeled anti-ICAM-1 antibody or PE labeled anti-E-selectin antibody. The EDC/NHS mixture forms an active ester intermediate with the -COOH groups of PAA which undergoes amidation reaction with the -NH2 group in the FITC labeled anti-ICAM-1 and PE labeled anti-E-selectin antibodies to yield the GNR-antibody conjugates.…”

Section: Photoacousic Molecular Imaging Of Inflammatory Diseasementioning

confidence: 99%

Photoacousic Molecular Imaging of Inflammatory Disease

2015

International Academy of Engineers (IA-E) April 24-25, 2015 Pattaya (Thailand)

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Abstract---Multiple biomarkers of cardiovascular inflammatory were simultaneously imaged in vivo using antibody conjugated gold nanorods (GNRs) injected into a mouse model of vascular injury stimulated by a photochemical reaction of Rose Bengal dye to green light. Mixed solutions of ICAM-1 antibody conjugated GNRs (715 nm) and E-selectin antibody conjugated GNRs (800 nm) were injected to bind to their respective inflammatory markers on the luminal surface of the inferior vena cava of a mouse. Photoacoustic intensity was measured by a commercial ultrasound probe synchronized to a pulsed laser (10-18 mJ/cm 2 ) at 715 nm or 800 nm clearly identified the up-regulation of targeted biomarkers. Histopathology on the harvested tissues confirmed inflammation. The feasibility of simultaneous photoacoustic molecular imaging of inflammation responses in cardiovascular system using a commercial ultrasound system has been demonstrated in vivo.

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Photoacoustic imaging of early inflammatory response using gold nanorods

Cited by 157 publications

References 21 publications

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

Picomolar sensitivity MRI and photoacoustic imaging of cobalt nanoparticles

Discrete dipole approximation simulations of gold nanorod optical properties: Choice of input parameters and comparison with experiment

Photoacousic Molecular Imaging of Inflammatory Disease

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