Photoacoustic imaging in evaluating early intestinal ischemia injury and reperfusion injury in rat models

Wang, Rui; Pan, Teng; Huang, Lin; Liao, Chengde; Li, Qinqing; Jiang, Huabei; Yang, Jun

doi:10.21037/qims-20-1160

Cited by 3 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the usefulness of PAI as an objective and quantitative evaluation method after ischemia and reperfusion has been widely reported in various organs including intestine 20 , 22 , 23 , and the feasibility of evaluating oxygenation by PAI has been established 24 .…”

Section: Discussionmentioning

confidence: 99%

“…With this method, oxygenation of the layers including the mucosal and submucosal layers can be evaluated noninvasively. In principle, oxygenation evaluation can be performed within tens of milliseconds, in other words, motion artifacts are almost negligible unlike computed tomography or magnetic resonance imaging 20 , 30 . Intraoperative guidance by PAI is greatly awaited in various fields 31 .…”

Section: Discussionmentioning

confidence: 99%

“…There are several reports of the usefulness of PAI in assessing intestinal viability 19 – 21 . Recently, Wang et al reported the usefulness of PAI for detecting early intestinal ischemia–reperfusion injury in rats 20 . They reported that, in a model of superior mesenteric artery embolism, there was a significant increase of PAI signal intensity in the group with a certain degree of damage compared to the control group.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Predicting intestinal viability by consecutive photoacoustic monitoring of oxygenation recovery after reperfusion in acute mesenteric ischemia in rats

Sugiura

Okumura

Matsumoto

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The purpose was to assess whether consecutive monitoring of oxygenation by photoacoustic imaging (PAI) can objectively predict intestinal viability during surgery for acute mesenteric ischemia (AMI). PAI uses laser light to detect relative amounts of oxygenated and deoxygenated hemoglobin in intestinal tissue. In 30 rats, AMI was induced by clamping the mesenteric and marginal vessels of the ileum for 0 min in the control group, 30 min in the mild group, and 180 min in the severe group (10 rats per group). After 60 min of reperfusion, intestinal damage was evaluated pathologically. Oxygenation of the intestine was monitored throughout the procedure in real time by a commercially available PAI system and compared among the groups. All rats showed irreversible (i.e. transmucosal or transmural infarction) damage in the severe group. After reperfusion, the oxygenation in the mild group recovered immediately and was significantly higher than in the severe group at 1, 5, 10, 30, and 60 min (P = .011, 002, < .001, 001, and 001, respectively). Oxygenation showed a significant strong negative correlation with pathological severity (rs = − 0.7783, − 0.7806, − 0.7422, − 0.7728, and − 0.7704, respectively). In conclusion, PAI could objectively predict irreversible ischemic damage immediately after reperfusion, which potentially prevents inadequate surgery.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Predicting intestinal viability by consecutive photoacoustic monitoring of oxygenation recovery after reperfusion in acute mesenteric ischemia in rats

Sugiura

Okumura

Matsumoto

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

Optical fiber-based handheld polarized photoacoustic computed tomography for detecting anisotropy of tissues

Ren¹,

Zhang²,

He³

et al. 2022

Quant Imaging Med Surg

View full text Add to dashboard Cite

Background: Photoacoustic computed tomography (PACT) is a fast-developing biomedical imaging modality and has immense potential for clinical translation. It utilizes laser excitation and acoustic detection to achieve high spatial resolution and considerable imaging depth in biological tissues. Current PACT primarily treats the absorption coefficient of tissues as a scalar variable while reconstructing the image, which limits its use for anisotropic evaluation of the tissues. Thus, by incorporating polarized imaging methods to evaluate anisotropy, applications of PACT can be further enhanced. So far, dichroism-sensitive PACT has been suggested for polarization detection of biological tissues. However, this approach is unsuitable for intraoperative imaging, since high-power spatial light is needed for excitation, which is dangerous and inconvenient to operate. Thus, there is a need to develop a polarized PACT system suitable for clinical use.Methods: Herein, we have proposed a specially designed handheld polarized PACT (HP-PACT) system, which was designed to promote intraoperative anisotropy detection of biological tissues. Excitation light was delivered by an optical fiber and reshaped by a compact set of lenses at the output end of the optical fiber. A polarizer was applied to generate linearly polarized light, and the polarization direction was adjusted by simply rotating the half-wave plate. Photoacoustic imaging (PAI) using excitation with several different polarization directions was carried out. Optical axes and the structure of the anisotropic objects were obtained using the principle of polarization detection with the PAI.Results: We experimentally demonstrated the performance of HP-PACT by imaging both the polarized and unpolarized plastic films. The results showed that HP-PACT can successfully detect the direction of the optical axes of polarized plastic films and has the ability to image at different depths. When linearly polarized light with different polarization directions was used as excitation, PAI studies on a highly anisotropic bovine tendon and relatively low anisotropic mouse leg showed the structural differences between the 2 tissues. The quantified degrees of anisotropy of the bovine tendon and mouse legs were 0.6 and 0.3, respectively. Conclusions:The proposed HP-PACT is able to determine the anisotropic substances' optical axes and distinguish anisotropic substances from isotropic ones. Thus, HP-PACT has the potential for intraoperative diagnosis and treatment of anisotropic tissues, including nerves and tendons.

show abstract

Activatable Small Molecule Probes for Photoacoustic Imaging: Dyes and Applications

Merkes

Kießling

Banala

2022

CMC

View full text Add to dashboard Cite

Photoacoustic imaging (PAI) is a non-invasive modality for molecular imaging and on the way to become a routine clinical diagnostic tool. The advantage of PAI over many other currently used modalities is its ability to potentially image in vivo a variety of enzymatic and physiological processes as well as metabolites in real time at high tissue depths. For this purpose, photoacoustic signal generating chromophores, which have the ability to change their signal characteristics upon reaction to their environments or trapping reactive species, are important. This review article provides an overview of the concepts for activateable small molecule probes for photoacoustic imaging, highlights the requirements on structural and optical properties, and describes their responses to the selective triggers.

show abstract

Photoacoustic imaging in evaluating early intestinal ischemia injury and reperfusion injury in rat models

Cited by 3 publications

References 41 publications

Predicting intestinal viability by consecutive photoacoustic monitoring of oxygenation recovery after reperfusion in acute mesenteric ischemia in rats

Predicting intestinal viability by consecutive photoacoustic monitoring of oxygenation recovery after reperfusion in acute mesenteric ischemia in rats

Optical fiber-based handheld polarized photoacoustic computed tomography for detecting anisotropy of tissues

Activatable Small Molecule Probes for Photoacoustic Imaging: Dyes and Applications

Contact Info

Product

Resources

About