Photoacoustic detection of SARS-CoV-2 spike N501Y single-nucleotide polymorphism based on branched rolling circle amplification

On-Particle Hyperbranched Rolling Circle Amplification-Scaffolded Magnetic Nanoactuator Assembly for Ferromagnetic Resonance Detection of MicroRNA

“…The high level of sequence homology for miRNAs from the same family makes specific quantification challenging. Guaranteed by the specificity of ligase, PLP ligation-based strategies are highly discriminative to mutations located at the termini of PLP, which has been exploited to distinguish single-nucleotide variants. , However, for mutations far from the termini of annealed PLP, the specificity should be compromised. As a comparison, PCR detects mutations based on the differences in melting temperatures between matched and mismatched probes (or primers), which is less single-nucleotide discriminative but more universal than PLP ligation-based methodologies.…”

AND-Logic Cascade Rolling Circle Amplification for Optomagnetic Detection of Dual Target SARS-CoV-2 Sequences

“…Owing to its high efficiency, simplicity, and robustness, rolling circle amplification (RCA) is the most widely adopted isothermal nucleic acid amplification method , and has been employed in DNA computation systems as the AND-logic operator or the output amplifier. − However, since the molecular amplification is not embedded before the operator, the output signal is strongly influenced by the bias between the two inputs (i.e., the weaker rather than the stronger input determines the output), leading to underestimations or false-negative judgments when the reference input is occasionally lower than the target input. In our previous studies, target molecule-induced cascade RCA reactions were designed to construct biosensors, − which suggest an “amplifiers-before-operator” configuration for ultrasensitive AND-logic gate quantification.…”