Abstract:Recently, intensive research on photoacoustic (PA) imaging has been conducted to accelerate the development of dermoscopy of the human skin. In this Letter, we first developed a PA dermoscope equipped with a waterless coupling and impedance matching opto-sono probe to achieve quantitative, high-resolution, and high-contrast imaging of the human skin. Compared with the commonly used liquid-coupled PA probes, the human-skin-adapted probe can facilitate implementation in the clinical setting. The noninvasive imag… Show more
“…Also various contrast agents can be used to enhance the visibility of internal structures [ 20 , 21 ] or in combination with treatment, to realize the theranostic approach [ [22] , [23] , [24] ]. Current promising applications of OA angiography include superficial tumor diagnostics in clinical oncology [ [25] , [26] , [27] ] and studies of tumor vascular pattern during growth and after the treatment of preclinical tumor models in experimental oncology [ 20 , 22 , [28] , [29] , [30] , [31] , [32] ].…”
Raster-scan optoacoustic angiography at 532 nm wavelength with 50 μm lateral resolution at 2 mm diagnostic depth was used for quantitative characterization of neoangiogenesis in colon cancer models. Two tumor models of human colon adenocarcinoma (HT-29) and murine colon carcinoma (CT26) different in their histology and vascularization were compared. Tumors of both origins showed an inhomogeneous distribution of areas with high and low vascularization. Rapidly growing CT26 tumor demonstrated a higher rate of vessel growth from the periphery to the center. Peculiarities of the vascularity of tumor models revealed by optoacoustic imaging were confirmed by fluorescent microscopy with FITC-dextran and morphological analysis. The obtained results may be important for the investigation of tumor development and for improvement of colon cancer treatment strategies.
“…Also various contrast agents can be used to enhance the visibility of internal structures [ 20 , 21 ] or in combination with treatment, to realize the theranostic approach [ [22] , [23] , [24] ]. Current promising applications of OA angiography include superficial tumor diagnostics in clinical oncology [ [25] , [26] , [27] ] and studies of tumor vascular pattern during growth and after the treatment of preclinical tumor models in experimental oncology [ 20 , 22 , [28] , [29] , [30] , [31] , [32] ].…”
Raster-scan optoacoustic angiography at 532 nm wavelength with 50 μm lateral resolution at 2 mm diagnostic depth was used for quantitative characterization of neoangiogenesis in colon cancer models. Two tumor models of human colon adenocarcinoma (HT-29) and murine colon carcinoma (CT26) different in their histology and vascularization were compared. Tumors of both origins showed an inhomogeneous distribution of areas with high and low vascularization. Rapidly growing CT26 tumor demonstrated a higher rate of vessel growth from the periphery to the center. Peculiarities of the vascularity of tumor models revealed by optoacoustic imaging were confirmed by fluorescent microscopy with FITC-dextran and morphological analysis. The obtained results may be important for the investigation of tumor development and for improvement of colon cancer treatment strategies.
“…After linearly scaling the laser pulse energy to 40 nJ without considering the non-linear effect, an averaged signal-to-noise ratio of 100 dB was estimated. Other integrated systems for performing OR-PAM have been reported 28,29 .Figure 7( a ) schematic of the photoacoustic imaging system and ( b ) its imaging head.…”
Ovarian cancer is the leading cause of death among gynecological cancers, but is poorly amenable to preoperative diagnosis. In this study, we investigate the feasibility of “optical biopsy,” using high-optical-resolution photoacoustic microscopy (OR-PAM) to quantify the microvasculature of ovarian and fallopian tube tissue. The technique is demonstrated using excised human ovary and fallopian tube specimens imaged immediately after surgery. Quantitative parameters are derived using Amira software. The parameters include three-dimensional vascular segment count, total volume and length, which are associated with tumor angiogenesis. Qualitative results of OR-PAM demonstrate that malignant ovarian tissue has larger and more tortuous blood vessels as well as smaller vessels of different sizes, while benign and normal ovarian tissue has smaller vessels of uniform size. Quantitative analysis shows that malignant ovaries have greater tumor vessel volume, length and number of segments, as compared with benign and normal ovaries. The vascular pattern of benign fallopian tube is different than that of benign ovarian tissue. Our initial results demonstrate the potential of OR-PAM as an imaging tool for fast assessment of ovarian tissue and fallopian tube and could avoid unnecessary surgery if the risk of the examined ovary is extremely low.
“…The PAI sub-system (in the green dotted box) consists of a microchip pulsed laser (Model DTL-314QT, pulsed Q-switched Laser, Russia) with a center wavelength of 532 nm and a pulse width of 10 ns as the excitation source for the PA signal of the sample. The PA signal is detected by a custom-made ultrasonic transducer (Doppler Electronic Technologies Co., Ltd., China) with central frequency of 25 MHz, which is mainly composed of an opto-sono polystyrene (PS) lens and a hollow bowl polyvinylidene fluoride (PVDF) element with a 1.5 mm center hole for the exit of the laser beam [33]. The OCT subsystem (in the red dotted box) consists of a low coherence light source (central wavelength of 1310 nm and spectral bandwidth of 45 nm) and an all-fiber Michelson interferometer (an optical circulator, a 2 × 2 fiber coupler and a photodetector).…”
Accurate and timely assessment of the severity of burn is essential for the treatment of burns. Currently, although most first-degree and thirddegree burns are easily diagnosed through visual inspection or auxiliary diagnostic methods, the seconddegree burn is still difficult to distinguish due to the ambiguity boundaries of second-degree with first-degree and third-degree burns. In this study, we proposed a non-invasive technique by combing photoacoustic imaging (PAI) and optical coherence tomography (OCT) to multi-parameter quantitatively assess the burns. The feasibility and capacity of the dualmode PAT/OCT for assessing the burns was first testified by tissuemimicking phantom and burn wounds in mouse pinna in vivo. The further
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