Photo-irradiation improved functional preservation of the isolated rat heart

Zhu, Qingyan; Yu, Wei; Yang, Xiaoping; Hicks, George L.; Lanzafame, Raymond J.; Wang, Ting‐Chung

doi:10.1002/(sici)1096-9101(1997)20:3<332::aid-lsm12>3.0.co;2-f

Cited by 40 publications

(25 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, LLLI precondition has a mitigating influence on the deleterious effects of the free radicals in the infarcted myocardium. Our findings are mainly in agreement with recent study by Zhu et al [38], who observed that LLLI at 660 nm wavelength improved functional recovery of the cold‐stored rat heart possibly via conservation of ATP and antioxidant enzyme activity. Although there is strong evidence of cardioprotective effects of LLLI, the underlying mechanisms remain unclear.…”

Section: Discussionsupporting

confidence: 93%

Low level laser irradiation precondition to create friendly milieu of infarcted myocardium and enhance early survival of transplanted bone marrow cells

Zhang

Hou

Shen³

et al. 2010

J Cellular Molecular Medi

View full text Add to dashboard Cite

We suggested that low-level laser irradiation (LLLI) precondition prior to cell transplantation might remodel the hostile milieu of infarcted myocardium and subsequently enhance early survival and therapeutic potential of implanted bone marrow mesenchymal stem cells (BMSCs). Therefore, in this study we wanted to address: (1) whether LLLI pre-treatment change the local cardiac micro-environment after myocardial infarction (MI) and (2) whether the LLLI preconditions enhance early cell survival and thus improve therapeutic angiogenesis and heart function. MI was induced by left anterior descending artery ligation in female rats. A 635 nm, 5 mW diode laser was performed with energy density of 0.96 J/cm2 for 150 sec. for the purpose of myocardial precondition. Three weeks later, qualified rats were randomly received with LLLI precondition (n= 26) or without LLLI precondition (n= 27) for LLLI precondition study. Rats that received thoracotomy without coronary ligation were served as sham group (n= 24). In the cell survival study, rats were randomly divided into 4 groups: serum-free culture media injection (n= 8), LLLI precondition and culture media injection (n= 8), 2 million male BMSCs transplantation without LLLI pre-treatment (n= 26) and 2 million male BMSCs transplantation with LLLI precondition (n= 25) group, respectively. Vascular endothelial growth factor (VEGF), glucose-regulated protein 78 (GRP78), superoxide dismutase (SOD) and malondialdehyde (MDA) in the infarcted myocardium were evaluated by Western blotting, real-time PCR and colorimetry, respectively, at 1 hr, 1 day and 1 week after laser irradiation. Cell survival was assayed with quantitative real-time PCR to identify Y chromosome gene and apoptosis was assayed with transferase-mediated dUTP end labelling staining. Capillary density, myogenic differentiation and left ventricular function were tested by immunohistochemistry and echocardiography, respectively, at 1 week. After LLLI precondition, increased VEGF and GRP78 expression, as well as the enhanced SOD activity and inhibited MDA production, was observed. Compared with BMSC transplantation and culture media injection group, although there was no difference in the improved heart function and myogenic differentiation, LLLI precondition significantly enhanced early cell survival rate by 2-fold, decreased the apoptotic percentage of implanted BMSCs in infarcted myocardium and thus increased the number of newly formed capillaries. Taken together, LLLI precondition could be a novel non-invasive approach for intraoperative cell transplantation to enhance cell early survival and therapeutic potential.

show abstract

Section: Discussionsupporting

confidence: 93%

Low level laser irradiation precondition to create friendly milieu of infarcted myocardium and enhance early survival of transplanted bone marrow cells

Zhang

Hou

Shen³

et al. 2010

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…It can be assumed that this elevation reflects the increase of catalase in the myocar- dium at a short time interval after MI by LELI, as was previously shown for isolated cardiomyocytes. 12 This elevated antioxidant activity in the dog ischemic myocardium is probably one of the mechanisms inhibiting cardiomyocyte degeneration and asserting a cardioprotective effect on these cells in the ischemic zone. The inverse significant correlation between the level of catalase after MI and infarct size in the dogs further supports the assumption that the antioxidant level in the ischemic myocardium at the early phase after LAD occlusion plays a major role in the complex processes that eventually lead to scar formation.…”

Section: Discussionmentioning

confidence: 99%

“…11 The effects of LELI on cardiac cells have been studied to a limited extent. Zhu et al 12 recently showed that argon dye laser (660-nm wavelength) improves the functional recovery of cold-stored, isolated rat cardiomyo-cytes. In the present study, we investigated the possibility that LELI may also attenuate the process of scar tissue formation after chronic occlusion of the LAD in rats and dogs.…”

mentioning

confidence: 99%

Low-Energy Laser Irradiation Reduces Formation of Scar Tissue After Myocardial Infarction in Rats and Dogs

et al. 2001

View full text Add to dashboard Cite

Background-Low-energy laser irradiation (LELI) has been found to attenuate various biological processes in tissue culture and experimental animal models. The aim of the present study was to investigate the effect of LELI on the formation of scar tissue in experimentally induced chronic infarct in rats and dogs. Methods and Results-Myocardial infarction (MI) was induced in 50 dogs and 26 rats by ligation of the left anterior descending coronary artery. After induction of MI, the laser-irradiated (LI) group received laser irradiation (infrared laser, 803-nm wavelength) epicardially. Control MI-induced non-laser irradiated (NLI) dogs were sham-operated, and laser was not applied. All dogs were euthanized at 5 to 6 weeks after MI. Infarct size was determined by TTC staining and histology. The laser treatment (PϽ0.05) lowered mortality significantly, from 30% to 6.5%, after induction of MI. The infarct size in the LI dogs was reduced significantly (PϽ0.0001) (52%) compared with NLI dogs. Histological observation of the infarct revealed a typical scar tissue in NLI dogs and cellularity in most of the LI dogs. Only 14Ϯ3% of the mitochondria in the cardiomyocytes in the ischemic zone (4 hours after MI) of LI MI-induced rats were severely damaged, compared with 36Ϯ1% in NLI rats. Accordingly, ATP content in that zone was 7.6-fold (significantly) higher in LI than in NLI rats. Conclusions-Our observations indicate that epicardial LELI of rat and dog hearts after chronic MI caused a marked reduction in infarct size, probably due to a cardioprotective effect of the LELI. Key Words: ischemia Ⅲ myocardial infarction Ⅲ antioxidants Ⅲ lasers T he approach to acute myocardial infarction (MI) has moved in the past decade from simple monitoring of coronary events to aggressive interventions in the processes underlying coronary thrombosis. Cardiac repair after infarct is a complex process involving diverse inflammatory components, extracellular matrix remodeling, and responses of the cardiomyocytes to the ischemia. 1,2 The sequential events that take place in the myocardium after occlusion of the left anterior descending coronary artery (LAD) in experimental animals (including dogs) have been well documented. 1,2 After necrosis of the cardiomyocytes and a rather long inflammatory phase, the ischemic zone is subsequently replaced by fibrotic tissue.Many studies have been directed toward the use of drugs, growth factors, and various interventional technologies in reducing myocardial infarct size and improvement of heart function after MI in experimental animals and humans. For example, recombinant adenovirus-mediated transfer of genes encoding antioxidants to the myocardium has been demonstrated to attenuate after ischemic dysfunction in neonatal mice. 3 Novel approaches to enhancing angiogenesis in the ischemic myocardium by introducing growth factors (mainly of the vascular endothelial growth factor family) were adapted and found to have a beneficial effect on patients with severe angina. 4 Low-energy laser irradiation (LELI) has b...

show abstract

“…We speculate that LEVL evokes a cellular adaptation mechanism and calcium signaling, which might explain previously obtained results such as improving functional preservation of isolated rat hearts by low energy photoirradiation (31), reduction of infarct size of the hearts of dogs (68), and enhancing recovery of ischemic damage in cardiomyocytes (69).…”

Section: Effect Of Visible Light Irradiation On [Ca 2ϩmentioning

confidence: 99%

“…We chose cardiomyocytes as low energy photoirradiation has been found to improve heart preservation for transplantation (31). In addition, cardiomyocytes possess low levels of antioxidant enzymes, thus being more sensitive to light and ROS.…”

mentioning

confidence: 99%

Low Energy Visible Light Induces Reactive Oxygen Species Generation and Stimulates an Increase of Intracellular Calcium Concentration in Cardiac Cells

Lavi

Shainberg

Friedmann

et al. 2003

Journal of Biological Chemistry

164

View full text Add to dashboard Cite

Low energy visible light (LEVL) irradiation has beenshown to exert some beneficial effects on various cell cultures. For example, it increases the fertilizing capability of sperm cells, promotes cell proliferation, induces sprouting of neurons, and more. To learn about the mechanism of photobiostimulation, we studied the relationship between increased intracellular calcium ( Life on earth is entirely dependent upon the interaction of sunlight with cells especially in plant photosynthesis (1). Sunlight also has medical benefits, which have been exploited for over thousands of years in ancient Egypt, India, and China in treating skin diseases, psoriasis, vitiligo, and even cancer (2). Recent observations show that even low energy visible light (LEVL) 1 can serve as a medical tool. For example, LEVL increases the rate of wound healing (3), enhances the fertilizing capability of sperm cells (4), and increases the rate of healing bone defects (5). In vitro studies have found that LEVL increases proliferation of cells as fibroblasts (6), keratinocytes (7), and lymphocytes (8) and induces the respiratory burst in neutrophils (9). The mechanism of photobiostimulation by LEVL is still unclear. It has been suggested that reactive oxygen species (ROS), which can be produced by photosensitization of endogenous cell chromophores such as cytochromes (10) [Ca 2ϩ ] i followed by myosin phosphorylation and cell contractions (26). Growth factors and hormones were shown to stimulate ROS production, which were dependent on [Ca 2ϩ ] i rise (27). The relationship between ROS and [Ca 2ϩ ] i has been suggested to involve the redox-sensitive transcription factor N␤, which was found to change [Ca 2ϩ ] i homeostasis in response to changes in the redox state of thiol groups (28). The kinetics that characterize the [Ca 2ϩ ] i elevation have been shown to be an important parameter determining the kind of signal that will be evoked. Livingston et al. (29) showed that high concentrations of oxidants (Ͼ50 M) caused a sustained increase in [Ca 2ϩ ] i , whereas a transient increase in [Ca 2ϩ ] i was observed following administration of a low concentration of oxidants. More than a 4-fold increase in the [Ca 2ϩ ] i level was obtained in photodynamic treatment of mouse myeloma cells that had been enriched with exogenous photosensitizers before illumination, whereas only a slight increase in [Ca 2ϩ ] i was observed in irradiated cells without exogenous photosensitizers (30).Only a few works have dealt with calcium/ROS changes following LEVL irradiation. In this study, we have investigated * The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.§ The article describes research performed by Ronit Lavi as partial fulfillment of the requirements for her Ph.D. degree at Bar-Ilan University. To whom correspondence should be addressed. Tel.: 972-3-5317797; Fax: 972-3-...

show abstract

Photo-irradiation improved functional preservation of the isolated rat heart

Cited by 40 publications

References 14 publications

Low level laser irradiation precondition to create friendly milieu of infarcted myocardium and enhance early survival of transplanted bone marrow cells

Low level laser irradiation precondition to create friendly milieu of infarcted myocardium and enhance early survival of transplanted bone marrow cells

Low-Energy Laser Irradiation Reduces Formation of Scar Tissue After Myocardial Infarction in Rats and Dogs

Low Energy Visible Light Induces Reactive Oxygen Species Generation and Stimulates an Increase of Intracellular Calcium Concentration in Cardiac Cells

Contact Info

Product

Resources

About