Photo-immunotargeting with haematoporphyrin conjugates activated by a low-power He-Ne laser

Berki, Tímea; Németh, Pèter

doi:10.1007/bf01741058

Cited by 14 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the sagittal axis, mobility (defined as the difference between the chin-jugulum distance on maximal reclination and inclination) improved during the course of treatment by a mean of 4.8 cm to reach 15.3 (12)(13)(14)(15)(16)(17)(18)(19)(20) cm in Group 1 and by 5.2 cm to reach 15.9 (12-18) cm in Group 2. In the axial and frontal planes, Group 1 showed an improvement of rotation (sum of right and left rotation in angular degrees) by 44.3° to reach 151.2° (120-166°) and an improvement of lateral bending (sum of right and left bending in angular degrees) by 14.4° to reach 70.4°( 58-76°), compared with improvements of 48° to reach 153.7° (119-170°) and of 18° to reach 74.4° (58-80°) in Group 2 (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…The use of lowlevel laser therapy (LLLT) for the treatment of musculoskeletal pain syndromes has also become common, particularly for tennis elbow (epicondylitis radialis humeri). However, the results of studies on LLLT vary greatly [12][13][14][15]; most authors have irradiated the affected region [16], but others irradiate the acupuncture points known from traditional Chinese medicine [17,18].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adjuvant laser acupuncture in the treatment of whiplash injuries: a prospective, randomized placebo-controlled trial

Aigner

Fialka²,

Radda

et al. 2006

Wien Klin Wochenschr

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Adjuvant laser acupuncture in the treatment of whiplash injuries: a prospective, randomized placebo-controlled trial

Aigner

Fialka²,

Radda

et al. 2006

Wien Klin Wochenschr

View full text Add to dashboard Cite

show abstract

“…These data further revealed that SOD activity in tissue is increased after low power laser stimulation but decreased after increased power [11]. Cell destruction was found to be dependent on the dose of He-Ne laser light energy [12], and biological membranes seem to be one of the primary targets [5]. In general, damage to the cellular mechanism as a result of laser irradiation is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Hairless mouse epidermal antioxidants and lipid peroxidation assessed by He‐Ne laser

Kim¹,

Pak²,

Lee³

2000

Lasers Surg. Med.

View full text Add to dashboard Cite

Background and Objective:To evaluate the possibility of oxygen radical damage in the skin after He-Ne laser irradiation according to dose intensity and time. Study Design/Materials and Methods: The He-Ne laser ( = 632.8, 10 mW) was used on the skin of mice with 1, 3, and 5 joule (J/cm 2 ) dose rates for 1, 5, and 7 days in each case, and the results were compared with normal and anesthetic nonirradiated skins. The efficacy was determined by the formation of thiobarbituric acid-reactive substances (TBARS) in a 10-minute period and expressed as a concentration of malondialdehyde (MDA) from the lipid peroxidation of epidermal tissue, and total superoxide dismutases (SODs) and catalase activities, correlated with histologic biopsies. Results: Data from epidermal SODs, catalase activity and the degree of lipid peroxidation at low-power radiation showed that repeated exposure had led to the induction of free radical damage and of epidermal changes as confirmed by microscopic study. The application of the HeNe laser at 1, 3, and 5 J intensity for 5 days caused a gradual increase in the SODs and catalase activities, while the levels of TBARS were slightly decreased in the mouse epidermis. However, these patterns were reversed after 3 and 5 J irradiations for 5 and 7 days laser treatment. Furthermore, microscopic examinations revealed that the laserirradiated skin changed the release of stratum granule from epidermis to hair follicle, and produced blood vessel thrombosis of the dermal capillary plexus. Conclusion:The presence of lipid peroxidation in the hairless mouse skin after exposure to He-Ne laser energy intensity of over 3 J for over 5 days was demonstrated. This lipid peroxidation could have been generated from oxygen free radicals. The histologic and oxidative enzymatic correlations on lipid peroxidation in the skin have provided a better understanding of He-Ne laser therapy-tissue interactions. It is possible to take advantage of these findings to evaluate pathologic skin conditions and effective laser dosage more efficiently. Lasers Surg. Med. 27:420-426, 2000.

show abstract

“…In vitro tests showed that the CAMAL-1:hematoporphyrin conjugate was able to efficiently kill CAMAL expressing cells when irradiated with a 620 nm dye laser. A similar conjugation method was followed by Németh et al, in 1991 ( Berki and Németh, 2005 ) and Pass et al, in 1993 ( Pogrebniak et al, 1993 ) to treat human gastric cancer and lung cancer, respectively, in athymic mice. Further, Németh et al, in 1998 again used the same carbodiimide conjugation strategy to generate a-Thy-1-hematoporphyrin antibody targeting Thy-l antigen in T lymphocytes to show the selective killing of T lymphocytes by the phototoxic conjugate upon irradiation ( Berki and Németh, 1998 ).…”

Section: Conjugation To Proteinsmentioning

confidence: 97%

Synthesis and Applications of Porphyrin-Biomacromolecule Conjugates

et al. 2021

View full text Add to dashboard Cite

With potential applications in materials and especially in light-responsive biomedicine that targets cancer tissue selectively, much research has focused on developing covalent conjugation techniques to tether porphyrinoid units to various biomacromolecules. This review details the key synthetic approaches that have been employed in the recent decades to conjugate porphyrinoids with oligonucleotides and peptides/proteins. In addition, we provide succinct discussions on the subsequent applications of such hybrid systems and also give a brief overview of the rapidly progressing field of porphyrin-antibody conjugates. Since nucleic acid and peptide systems vary in structure, connectivity, functional group availability and placement, as well as stability and solubility, tailored synthetic approaches are needed for conjugating to each of these biomacromolecule types. In terms of tethering to ONs, porphyrins are typically attached by employing bioorthogonal chemistry (e.g., using phosphoramidites) that drive solid-phase ON synthesis or by conducting post-synthesis modifications and subsequent reactions (such as amide couplings, hydrazide-carbonyl reactions, and click chemistry). In contrast, peptides and proteins are typically conjugated to porphyrinoids using their native functional groups, especially the thiol and amine side chains. However, bioorthogonal reactions (e.g., Staudinger ligations, and copper or strain promoted alkyne-azide cycloadditions) that utilize de novo introduced functional groups onto peptides/proteins have seen vigorous development, especially for site-specific peptide-porphyrin tethering. While the ON-porphyrin conjugates have largely been explored for programmed nanostructure self-assembly and artificial light-harvesting applications, there are some reports of ON-porphyrin systems targeting clinically translational applications (e.g., antimicrobial biomaterials and site-specific nucleic acid cleavage). Conjugates of porphyrins with proteinaceous moieties, on the other hand, have been predominantly used for therapeutic and diagnostic applications (especially in photodynamic therapy, photodynamic antimicrobial chemotherapy, and photothermal therapy). The advancement of the field of porphyrinoid-bioconjugation chemistry from basic academic research to more clinically targeted applications require continuous fine-tuning in terms of synthetic strategies and hence there will continue to be much exciting work on porphyrinoid-biomacromolecule conjugation.

show abstract

Photo-immunotargeting with haematoporphyrin conjugates activated by a low-power He-Ne laser

Cited by 14 publications

References 23 publications

Adjuvant laser acupuncture in the treatment of whiplash injuries: a prospective, randomized placebo-controlled trial

Adjuvant laser acupuncture in the treatment of whiplash injuries: a prospective, randomized placebo-controlled trial

Hairless mouse epidermal antioxidants and lipid peroxidation assessed by He‐Ne laser

Synthesis and Applications of Porphyrin-Biomacromolecule Conjugates

Contact Info

Product

Resources

About