Photo‐Decomposable Sub‐Micrometer Albumin Particles Cross‐Linked by ortho‐Nitrobenzyl Derivatives

Abstract

Stimuli‐responsive particles are widely used as carriers for on‐demand delivery of drugs, genes, proteins, etc., due to their response to environmental stimuli. In this study, photoresponsive albumin sub‐micrometer particles are fabricated by using ortho‐nitrobenzyl derivative 4‐bromomethyl‐3‐nitrobenzoic acid (BNBA) as a cross‐linker. Bovine serum albumin (BSA)‐doped MnCO3 particles are used as the sacrificial template, in which the BSA molecules are cross‐linked by BNBA under the activation of 4‐(4,6‐dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride. After removal of MnCO3 particles by ethylene diamine tetraacetic acid, photoresponsive BSA–BNBA sub‐micrometer particles are obtained. Since the CN bond formed via reaction of benzyl bromide and amine is photocleavable, the particles can be decomposed upon UV irradiation at 365 nm under acidic environment. The loaded macromolecules can be released upon UV irradiation, exhibiting great potential applications of the particles in the field of controlled release.

Biopolymer-Based Multilayer Capsules and Beads Made via Templating: Advantages, Hurdles and Perspectives

“…BSA particles can be achieved. The cross-linking of BSA with ortho-nitrobenzyl derivative 4-bromomethyl-3-nitrobenzoic acid under activation with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride, followed by MnCO 3 dissolution, results in particulate BSA, which contains photo-cleavable C-N bonds, allowing for subsequent UV-controlled release of the cargo [186].…”

Synthesis and characterization of enzymatically active micrometer protein-capsules

“…ACCs were created by a method first presented by Xiong et al [5,19,20] and later modified by Schakowski et al [4]. Crosslinking of the BSA molecules by genipin as described in previous works [4,[21][22][23] results in a stable shell of BSA around the core particle.…”