Photo‐Crosslinked Gelatin‐Based Hydrogel Films to Support Wound Healing

Abstract

Gelatin is used widely in the biomedical field, among other for wound healing. Given its upper critical solution temperature, crosslinking is required. To this end, gelatin is chemically modified with different photo-crosslinkable moieties with low (32-34%) and high (63-65%) degree of substitution (DS): gelatin-methacrylamide (gel-MA) and gelatin-acrylamide (gel-AA) and gelatin-pentenamide (gel-PE). Next to the more researched gel-MA, it is especially interesting and novel to compare with other gelatin-derived compounds for the application of wound healing. An additional comparison is made with commercial dressings. The DS is directly proportional to the mechanical characteristics and inversely proportional to the swelling capacity. Gel-PE shows weaker mechanical properties (G′ < 15 kPa) than gel-AA and gel-MA (G′ < 39 and 45 kPa, respectively). All derivatives are predominantly elastic (recovery indices of 89-94%). Gel-AA and gel-MA show excellent biocompatibility, whereas gel-PE shows a significantly lower initial biocompatibility, evolving positively toward day 7. Overall, gel-MA shows to have the most potential to be applied as wound dressing. Future blending with gel-AA to improve the curing kinetics can lead to dressings able to compete with current commercial dressings.

Lyophilized Platelet-Rich Fibrin Exudate-Loaded Carboxymethyl Chitosan/GelMA Hydrogel for Efficient Bone Defect Repair

“…The changes in rheological properties, especially the increase in storage modulus (G′), can reflect the crosslinking process and density of the polymer network. 57 As shown in Figure S3, G′ exceeded the loss modulus (G″) upon applying light irradiation in all four groups, indicating the successful formation of gel networks. Besides, G′ of all groups reached plateaus within 120 s of light exposure.…”

“…The near-UV blue light-initiated crosslinking of precursor solutions was studied in situ using rheology. The changes in rheological properties, especially the increase in storage modulus ( G ′), can reflect the crosslinking process and density of the polymer network . As shown in Figure S3, G ′ exceeded the loss modulus ( G ″) upon applying light irradiation in all four groups, indicating the successful formation of gel networks.…”

Reactive Oxygen Species Suppressive Kraft Lignin‐Gelatin Antioxidant Hydrogels for Chronic Wound Repair

“…[49][50][51][52] It is applied in various wound healing applications due to its promising biological properties. [53][54][55][56][57][58][59] However, chemical modifications of gelatin are necessary to enhance its thermo-reversible behavior under physi-ological temperature, and mechanical properties for a successful tissue regeneration. [60][61][62] Herein, an antioxidant hydrogel was developed through chemical cross-linking kraft lignin with gelatin for a chronic wound repair application.…”

“…[ 49–52 ] It is applied in various wound healing applications due to its promising biological properties. [ 53–59 ] However, chemical modifications of gelatin are necessary to enhance its thermo‐reversible behavior under physiological temperature, and mechanical properties for a successful tissue regeneration. [ 60–62 ]…”

Droplet absorption and spreading into thin layers of polymer hydrogels

“…In tissue engineering, they are used as a matrix framework for the repair and regeneration of a wide range of tissues and organs [16][17][18]. Hydrogel wound dressings have been developed that maintain a moist healing environment whilst allowing gaseous exchange, promoting wound healing [4,19,20]. These systems, characterised by complex interactions between biological materials and hydrogels, require a thorough understanding of hydrogel swelling behaviour.…”