Photo-chemically patterned polymer surfaces for controlled PC-12 adhesion and neurite guidance

Welle, Alexander; Horn, S.; Schimmelpfeng, Jutta; Kalka, Dorothee

doi:10.1016/j.jneumeth.2004.08.011

Cited by 61 publications

(52 citation statements)

References 39 publications

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“…High-energy light, such as deep UV light below 200nm (Azioune et al, 2010) or concentrated light from pulsed lasers (see panel C) (Doyle et al, 2009), have also proved useful to create local plasma and directly oxidise culture substrates without the need for dedicated photo-chemistry. Exposure to plasma renders hydrophobic polystyrene culture substrates hydrophilic (Welle and Gottwald, 2002;Welle et al, 2005) and also destroys the protein and cell repellent properties of PEG (Azioune et al, 2009), thus allowing for further protein grafting.Most of these methods can be repeated to micropattern several distinct proteins at specific locations (multi-patterning), as long as each step preserves the preceding protein coating. Repeated micro-contact printing steps are difficult to perform, as they require to align all printing steps.…”

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confidence: 99%

Micropatterning as a tool to decipher cell morphogenesis and functions

Théry

2010

Journal of Cell Science

646

560

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Cell architectureThe physiological cell microenvironment consists of extracellular matrix (ECM) fibres, adjacent cells and extracellular fluids, and the cell adhesion machinery is the first cellular component to encounter it. Upon binding of extracellular ligands, localised signalling is induced with subsequent assembly of the cytoskeleton. These localised events will affect the entire cell architecture, because the intracellular space is physically connected and SummaryIn situ, cells are highly sensitive to geometrical and mechanical constraints from their microenvironment. These parameters are, however, uncontrolled under classic culture conditions, which are thus highly artefactual. Micro-engineering techniques provide tools to modify the chemical properties of cell culture substrates at sub-cellular scales. These can be used to restrict the location and shape of the substrate regions, in which cells can attach, so-called micropatterns. Recent progress in micropatterning techniques has enabled the control of most of the crucial parameters of the cell microenvironment. Engineered micropatterns can provide a micrometer-scale, soft, 3-dimensional, complex and dynamic microenvironment for individual cells or for multi-cellular arrangements. Although artificial, micropatterned substrates allow the reconstitution of physiological in situ conditions for controlled in vitro cell culture and have been used to reveal fundamental cell morphogenetic processes as highlighted in this review. By manipulating micropattern shapes, cells were shown to precisely adapt their cytoskeleton architecture to the geometry of their microenvironment. Remodelling of actin and microtubule networks participates in the adaptation of the entire cell polarity with respect to external constraints. These modifications further impact cell migration, growth and differentiation. Journal of Cell Sciencemechanically supported by a dynamic equilibrium (Ingber, 2003; Ingber, 2006). Integrin-based cell adhesionIntegrins are transmembrane receptors that bind to ECM proteins and to intracellular actin filaments. When cells contact the ECM, they change their shape and spread in a multi-step process that includes cell attachment, formation of membrane protrusion, extension of cell membrane, and formation and contraction of stress fibers, which further stimulate cell attachment, membrane protrusion and cell shape extension.The attachment of the actin cytoskeleton to cell adhesions requires integrin clustering. Nanopatterning methods have led to determine the maximum distance of 60 nm between integrin molecules -a distance that still allows intracellular recruitment of actin filaments and signalling molecules (Arnold et al., 2004). Arrays of adhesive dots have been used to study the formation of filopodia and subsequent spreading steps. Depending on the cell type and the level of Rac activation, cells need a minimal distance between adhesion sites, so that filopodia can bridge them and promote cell spreading (Guillou et al., 2008;Lehnert et al., 2004). Th...

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confidence: 99%

Micropatterning as a tool to decipher cell morphogenesis and functions

Théry

2010

Journal of Cell Science

646

560

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“…It is well established that the microenvironment of a cell greatly influences its state of differentiation, and one of the great challenges of tissue engineering has been the precise control of cell-cell contacts. 185 One classic example is the primary hepatocyte, which rapidly loses a number of hepatocytespecific markers, such as cytochrome P-450 186 , albumin, and urea 187 once removed from its natural environment. This loss of function can be ameliorated by coculture with nonparenchymal cells.…”

Section: Cell Patterning and Directed Growthmentioning

confidence: 99%

Light forces the pace: optical manipulation for biophotonics

2010

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Abstract. The biomedical sciences have benefited immensely from photonics technologies in the last 50 years. This includes the application of minute forces that enable the trapping and manipulation of cells and single molecules. In terms of the area of biophotonics, optical manipulation has made a seminal contribution to our understanding of the dynamics of single molecules and the microrheology of cells. Here we present a review of optical manipulation, emphasizing its impact on the areas of single-molecule studies and single-cell biology, and indicating some of the key experiments in the fields.

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“…Functional studies have therefore been hindered by these ill-defined arrangements that do not accurately capture in vivo geometries and connectivities (49). To address this need, researchers have adapted microtechnologies for engineering substrates with defined biochemical patterns and topographical features.…”

Section: Microtechnologiesmentioning

confidence: 99%

“…Micropatterned Cues. Many methods exist for patterning substrates with biochemical cues (49). Microcontact printing is a common technique used to pattern substrates with a silicone rubber stamp ''inked'' with peptides, proteins, or other cell adhesion-promoting factors.…”

Section: Microtechnologiesmentioning

confidence: 99%

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Tissue‐Engineered Peripheral Nerve

Leach

2006

Wiley Encyclopedia of Biomedical Engineering

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Peripheral nerves can be injured by a variety of mechanisms but can regenerate on their own if the injury is not extensive. Nerve grafts, the current standard of surgical care for repairing large nerve gaps, are successful, but they can be associated with several major drawbacks. To overcome these disadvantages, researchers are designing “off‐the‐shelf” nerve guide implants; thus, a multidisciplinary effort is underway to engineer nerve repair through a deeper understanding of the dynamic cell–cell and cell–environment interactions that occur during regeneration. This entry, which focuses on literature published in the last 5 years, first emphasizes new nerve guide designs that have been demonstrated to improve functional regeneration in vivo . The second section reviews studies that have deepened our understanding of in vitro and in vivo neural regenerative responses via new biomaterials technologies and imaging techniques. These critical advancements in peripheral nerve tissue engineering provide templates for future mechanistic studies and improved strategies to aid functional regeneration in vivo .

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Photo-chemically patterned polymer surfaces for controlled PC-12 adhesion and neurite guidance

Cited by 61 publications

References 39 publications

Micropatterning as a tool to decipher cell morphogenesis and functions

Micropatterning as a tool to decipher cell morphogenesis and functions

Light forces the pace: optical manipulation for biophotonics

Tissue‐Engineered Peripheral Nerve

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