Abstract:Abstract. Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions provide valuable long-range structural information in NMR spectroscopic analyses of biological macromolecules such as proteins, but labelling proteins site-specifically with a single lanthanide ion remains an ongoing challenge, especially for proteins that are not suitable for ligation with cysteine-reactive lanthanide complexes. We show that a specific lanthanide binding site can be installed on proteins by incorporation of phospho… Show more
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