Phosphorylation of Tyrosine 1070 at the GluN2B Subunit Is Regulated by Synaptic Activity and Critical for Surface Expression of N-Methyl-d-aspartate (NMDA) Receptors

Increased Activity of Src Homology 2 Domain Containing Phosphotyrosine Phosphatase 2 (Shp2) Regulates Activity-dependent AMPA Receptor Trafficking

“…Extraction of Subcellular Fraction-Subcellular fractionation was performed as described (12). Hippocampus from adult mice or the CA1 region of the hippocampal slice was homogenized in cold buffer A (0.32 M sucrose, 10 mM HEPES, pH 7.4) and centrifuged at 1,000 ϫ g for 4 min to remove nuclei and large debris (P1).…”

Ca2+‐Dependent Hyperpolarization Pathways in Sleep Homeostasis and Mental Disorders

“…In this case, Process S is identified as an activity‐dependent effect (e.g., phosphorylation) and/or a mechanism affecting the quantity (e.g., translation) of target molecules (Figure B). Given the increasing evidence for the post‐translational modification of K Ca channels, Ca v channels, and NMDARs, one of the plausible execution mechanisms of Process S is activity‐dependent protein modifications (e.g., phosphorylation or dephosphorylation) of components of the fast and slow Ca 2+ ‐dependent hyperpolarization pathways. The finding that the impairment of CaMKIIα ( Camk2a ) and CaMKIIβ ( Camk2b ), which are well‐known to bind and modify Ca v channels and NMDARs, decreases sleep duration may support this mechanism.…”

Antidiabetic Drug Metformin Ameliorates Depressive-Like Behavior in Mice with Chronic Restraint Stress via Activation of AMP-Activated Protein Kinase

“…The procedure was performed as described in previously [34]. Briefly, the hippocampal tissues from mice were rapidly dissected and homogenized in ice-cold RIPA buffer containing 10 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% Triton X-100, and 1% SDS (Beyotime) with Protease Inhibitor and Phosphatase Inhibitor and centrifuged at 16,000 × g for 20 min.…”