Phosphorylation of the zinc finger transcriptional regulator ZAT6 by MPK6 regulates Arabidopsis seed germination under salt and osmotic stress

Liu, Xiaomin; Nguyen, Xuan Canh; Kim, Kyung Eun; Han, Hay Ju; Yoo, Jaehyeong; Lee, Kyung‐Hee; Kim, Min Chul; Yun, Dae‐Jin; Chung, Woo Sik

doi:10.1016/j.bbrc.2012.12.039

Cited by 87 publications

(68 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly to H 2 O 2 , ZAT12 stimulates the induction of the APX1 gene in response to light stress, providing a feedback regulatory circuit for H 2 O 2 removal (Davletova et al, 2005b). WRKY30 is induced by superoxide anions generated in damaged chloroplasts (Scarpeci et al, 2008), whereas ZAT6 is activated by osmotic and salt stress (Liu et al, 2013). These results suggest that HSFA4A can act in common pathways with these transcription factors.…”

Section: Hsfa4a-regulated Genesmentioning

confidence: 87%

“…MAP kinases were reported to phosphorylate other transcription factors such as WRKY33, which is involved in regulation of ethylene biosynthesis (Li et al, 2012), or MYB44, which regulates abscisic acid sensitivity (Nguyen et al, 2012b). MPK3 and MPK6 can form in vivo complexes with ZAT10/salt tolerance zinc finger, regulating plant defense responses Nguyen et al, 2012a), while under salt and osmotic stress, MPK6 interacts with and phosphorylates ZAT6 (Liu et al, 2013). Thus, MPK3/MPK6 appear to regulate a wide range of biotic and abiotic defense responses mediated by ROS signals and coordinate the activity of various transcription factors such as WRKY, MYB, ZAT, and HSF that, in turn, control the transcription of a large set of target genes.…”

Section: Phosphorylation Of Hsfa4a By Interacting Mpk3 and Mpk6 Kinasesmentioning

confidence: 99%

See 1 more Smart Citation

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

et al. 2014

View full text Add to dashboard Cite

Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradioldependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis.

show abstract

Section: Hsfa4a-regulated Genesmentioning

confidence: 87%

Section: Phosphorylation Of Hsfa4a By Interacting Mpk3 and Mpk6 Kinasesmentioning

confidence: 99%

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

et al. 2014

View full text Add to dashboard Cite

show abstract

“…to lack of protein phosphorylation, which is necessary for ZAT6 (Liu et al, 2013), ChIP assays showed that the ZAT6 protein can specifically bind to the promoter of GSH1 in plants. These results collectively demonstrate that GSH1 is a key target gene under transcriptional control of ZAT6.…”

Section: Discussionmentioning

confidence: 99%

Zinc-Finger Transcription Factor ZAT6 Positively Regulates Cadmium Tolerance through the Glutathione-Dependent Pathway in Arabidopsis

et al. 2016

View full text Add to dashboard Cite

“…is activated by osmotic stress in addition to salt (Liu, 2013), these results suggest that HSFA4A, as upstream regulator, acts in common pathways with WRKY30 and ZAT6.…”

Section: The Hsfa4a T-dna Mutant Is Hypersensitive To Saltmentioning

confidence: 71%

“…MAP kinases were shown to phosphorylate transcription factors such as WRKY33, involved in ethylene biosynthesis (Li, 2012), or MYB44 which regulates ABA sensitivity . MPK3 and MPK6 can form in vivo complexes with ZAT10/STZ , while under salt and osmotic stress, MPK6 interacts with and phosphorylates ZAT6 (Liu, 2013 (Wang, 2003).…”

Section: Phosphorylation Of Hsfa4a By Interacting Mpk3 and Mpk6mentioning

confidence: 99%

Functional characterization of the Arabidopsis heat shock factor A4A, identified by a novel genetic screen

Immaculada

View full text Add to dashboard Cite

Phosphorylation of the zinc finger transcriptional regulator ZAT6 by MPK6 regulates Arabidopsis seed germination under salt and osmotic stress

Cited by 87 publications

References 22 publications

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

The Heat Shock Factor A4A Confers Salt Tolerance and Is Regulated by Oxidative Stress and the Mitogen-Activated Protein Kinases MPK3 and MPK6

Zinc-Finger Transcription Factor ZAT6 Positively Regulates Cadmium Tolerance through the Glutathione-Dependent Pathway in Arabidopsis

Functional characterization of the Arabidopsis heat shock factor A4A, identified by a novel genetic screen

Contact Info

Product

Resources

About