Abstract:Purified protein samples are often acquired via recombinant DNA techniques using heterologous E. coli induced expression systems. This bacterial expression and purification technique has been widely utilized for decades, and many researchers have worked under the misconception that eukaryotic proteins are not post‐translationally modified in a significant way during expression and purification in bacteria. Based on contradicting experimental data, we hypothesized that cortactin is phosphorylated by endogenous … Show more
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