Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought

Chen, Qing-Chao; Hu, Tao; Li, Xiaohua; Song, Chun‐Peng; Zhu, Jian‐Kang; Chen, Liqing; Zhao, Yanping

doi:10.1038/s41477-021-01040-7

Cited by 127 publications

(106 citation statements)

References 45 publications

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“…Detailed research revealed that in response to drought in rice, OsSWEET13 and OsSWEET15 genes were activated because of an ABA‐responsive transcription factor OsbZIP72 directly binds to their promoters (Mathan et al., 2020). Another independent research found that Arabidopsis SWEET11 and SWEET12 are rapidly phosphorylated under the conditions of drought and abscisic acid treatments and this phosphorylation enhances the sucrose transport activity of SWEETs (Chen et al., 2022). However, the reason that some SWEET genes were downregulated under drought stress will need to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Comparison of SWEET gene family between maize and foxtail millet through genomic, transcriptomic, and proteomic analyses

Liu

2022

The Plant Genome

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Foxtail millet [Setaria italica (L.) P. Beauv.] does not show high yield and biomass compared with maize (Zea mays L.) although it is a C 4 crop with the potential for high productivity. Because SWEET genes, which are important for sugar transport in plants, play critical roles in biomass production and seed filling in crops, genome-wide, transcriptomic, and proteomic comparison on SWEET gene family between these two species would provide some clues for unlocking this issue. In our study, 24 SWEET genes were identified in foxtail millet and maize. Sequence-based bioinformatics combined with gene expression analyses identified several candidate functional orthologs in these two species. A comparative analysis on expression characteristics of SWEET genes and proteins between maize and foxtail millet indicate that not only some critical major SWEET proteins show significant upregulation in maize compared with their orthologs in foxtail millet, but also there are more quantities of maize SWEET genes showing high expressions than that of foxtail millet genes, suggesting that compared with foxtail millet, maize possesses higher capacity of sugar transport, the crucial determinant for crop yield and biomass. These results provide a basis on revealing why foxtail millet exhibits low yield and biomass although it is a C 4 crop with the potential for high productivity. INTRODUCTIONSucrose is not only the main end product of photosynthesis but also the predominant form of photosynthates transported by the phloem in higher plants (Volkert et al., 2014).

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Section: Introductionmentioning

confidence: 99%

Comparison of SWEET gene family between maize and foxtail millet through genomic, transcriptomic, and proteomic analyses

Liu

2022

The Plant Genome

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“…The transcript levels of the AtSWEET11, AtSWEET12 , and AtSUC2 genes are highly induced in the leaves of plants under water deficit conditions and these sucrose transporters were predicted to be involved in exporting sucrose accumulated in the leaves to the roots (Durand et al, 2016). In the recent detailed study the phosphorylation of sucrose transporters was shown to direct enhanced root:shoot ratio in plants under drought stress (Chen et al, 2022; Gong and Yang, 2022). In addition, AtSWEET11, AtSWEET12 , and AtSUC2 gene expression was also induced in water-stressed roots, indicating the potential role of these transporters during sucrose-unloading processes (Durand et al, 2016; Gong and Yang, 2022) ( Figure 2E ).…”

Section: Resultsmentioning

confidence: 99%

SWEET11 and SWEET12 transporters function in tandem to modulate sugar flux in Arabidopsis: An account of the underlying unique structure–function relationship

Fatima

Balasubramaniam

Khan³

et al. 2022

Preprint

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Sugar will eventually be exported transporters (SWEETs) have been identified as a unique class of sugar efflux transporters in all biological kingdoms. AtSWEET11 and AtSWEET12 in Arabidopsis act synergistically to perform distinct physiological roles, particularly in apoplasmic phloem loading, seed filling, and sugar level alteration at the site of pathogen infection. Plasma membrane-localized AtSWEET11 and AtSWEET12 transporters exclusively facilitate sucrose transport along the concentration gradient. This article examines the sucrose binding pocket of AtSWEET11 and AtSWEET12 using docking studies, and how they act synergistically in various functions throughout plant development and during abiotic and biotic stresses. Further, we highlight the phylogenetic and the in-silico analyses of AtSWEET11 and AtSWEET12 orthologs from 39 economically important plant species that could provide new platforms for future studies on sugar allocation mechanisms across the different plant families. An in-depth understanding of these transporters and their molecular regulatory mechanisms could be harnessed for crop improvement and crop protection.

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“…In line with the rapid growth of SWEETs research in Arabidopsis [ 2 , 13 , 14 , 28 , 29 ], rice [ 11 ], and maize [ 30 , 31 , 32 ], studies on SWEET homologues in other plant species such as tomato [ 33 ], watermelon [ 25 ], and the tea plant [ 15 , 16 ] have been reported, and findings have been updated. Although SWEETs are important versatile regulators and affect yield potential, only two cucumber CsSWEET genes have been reported in detail to date [ 21 , 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have demonstrated that AtSWEET11 and AtSWEET12 are directly phosphorylated by SnRK2 protein kinases, leading to enhanced drought resistance of Arabidopsis plants [ 29 ]. In rice, the expression of OsSWEET13 and OsSWEET15 is activated by the direct binding of their promoters to OsbZIP72 , an ABA-responsive transcription factor, resulting in the maintenance of sugar homeostasis and improved resistance to drought and salinity stresses [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

CsSWEET2, a Hexose Transporter from Cucumber (Cucumis sativus L.), Affects Sugar Metabolism and Improves Cold Tolerance in Arabidopsis

Zhang

Song

et al. 2022

IJMS

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Sugars, which are critical osmotic compounds and signalling molecules in plants, and Sugars Will Eventually be Exported Transporters (SWEETs), which constitute a novel family of sugar transporters, play central roles in plant responses to multiple abiotic stresses. In the present study, a member of the SWEET gene family from cucumber (Cucumis sativus L.), CsSWEET2, was identified and characterized. Histochemical analysis of β-glucuronidase expression in transgenic Arabidopsis plants showed that CsSWEET2 is highly expressed in the leaves; subcellular localization indicated that CsSWEET2 proteins are localized in the plasma membrane and endoplasmic reticulum. Heterologous expression assays in yeast demonstrated that CsSWEET2 encodes an energy-independent hexose/H+ uniporter that can complement both glucose and fructose transport deficiencies. Compared with wild-type Arabidopsis plants, transgenic Arabidopsis plants overexpressing CsSWEET2 had much lower relative electrolyte leakage levels and were much more resistant to cold stress. Sugar content analysis showed that glucose and fructose levels in the transgenic Arabidopsis plants were significantly higher than those in the wild-type plants. Taken together, our results suggest that, by mediating sugar metabolism and compartmentation, CsSWEET2 plays a vital role in improving plant cold tolerance.

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Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought

Cited by 127 publications

References 45 publications

Comparison of SWEET gene family between maize and foxtail millet through genomic, transcriptomic, and proteomic analyses

Comparison of SWEET gene family between maize and foxtail millet through genomic, transcriptomic, and proteomic analyses

SWEET11 and SWEET12 transporters function in tandem to modulate sugar flux in Arabidopsis: An account of the underlying unique structure–function relationship

CsSWEET2, a Hexose Transporter from Cucumber (Cucumis sativus L.), Affects Sugar Metabolism and Improves Cold Tolerance in Arabidopsis

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