Phosphorylation of plant virus proteins: Analysis methods and biological functions

Zhuang, Xinjian; Guo, Xiao; Gu, Tianxiao; Xu, Xiaowei; Qin, Lang; Xu, Kai; He, Zhen; Zhang, Kun

doi:10.3389/fmicb.2022.935735

Cited by 5 publications

(4 citation statements)

References 153 publications

(166 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These ndings demonstrate that the onset of puberty is regulated by phosphorylated proteins. PTMs act as signal transducers for adaptations in living organisms because of their reversible and rapid nature, relatively small energy requirement, and effective functional modulation of the target protein [34]. Phosphorylation is the most intensively investigated PTM and is involved in almost every cellular process to orchestrate a variety of cellular signal transduction processes [35].…”

Section: Discussionmentioning

confidence: 99%

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goatsto determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty. Results: We used peptide and posttranslational modifications peptide quantification and statistical analyses, and identified 67 differentially expressed proteins from 5,057 proteins and 576 differentially expressed phosphopeptides from 1574 phosphorylated proteins. Combined proteomic and phosphoproteomics, 759 correlated proteins were identified, of which 5 were differentially expressed only at the protein level, and 201 were only differentially expressed at the phosphoprotein level. Pathway enrichment analyses revealed that the majority of correlated proteins were associated with glycolysis/gluconeogenesis, Fc gamma R-mediated phagocytosis, focal adhesion, GABAergic synapse, and Rap1 signaling pathway. These pathways are related to cell proliferation, neurocyte migration, and promoting the release of gonadotropin-releasing hormone in the hypothalamus. CTNNB1 occupied important locations in the protein-protein interaction network and is involved in focal adhesion. Conclusion: The results demonstrate that the proteins differentially expression only at the protein level or only differentially expressed at the phosphoprotein level and their related signalling pathways are crucial in regulating puberty in goats. These differentially expressed proteins and phosphorylated proteins may constitute the proteomic backgrounds between the two different stages.

show abstract

Section: Discussionmentioning

confidence: 99%

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…These findings demonstrate that the onset of puberty is regulated by phosphorylated proteins. PTMs act as signal transducers for adaptations in living organisms because of their reversible and rapid nature, relatively small energy requirement, and effective functional modulation of the target protein [ 38 ]. Phosphorylation is the most intensively investigated PTM and is involved in almost every cellular process to orchestrate a variety of cellular signal transduction processes [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Ye,

Yan,

Zhang

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

Background Puberty marks the end of childhood and achieve sexual maturation and fertility. The role of hypothalamic proteins in regulating puberty onset is unclear. We performed a comprehensive differential proteomics and phosphoproteomics analysis in prepubertal and pubertal goats to determine the roles of hypothalamic proteins and phosphoproteins during the onset of puberty. Results We used peptide and posttranslational modifications peptide quantification and statistical analyses, and identified 69 differentially expressed proteins from 5,057 proteins and 576 differentially expressed phosphopeptides from 1574 phosphorylated proteins. Combined proteomic and phosphoproteomics, 759 correlated proteins were identified, of which 5 were differentially expressed only at the protein level, and 201 were only differentially expressed at the phosphoprotein level. Pathway enrichment analyses revealed that the majority of correlated proteins were associated with glycolysis/gluconeogenesis, Fc gamma R-mediated phagocytosis, focal adhesion, GABAergic synapse, and Rap1 signaling pathway. These pathways are related to cell proliferation, neurocyte migration, and promoting the release of gonadotropin-releasing hormone in the hypothalamus. CTNNB1 occupied important locations in the protein-protein interaction network and is involved in focal adhesion. Conclusion The results demonstrate that the proteins differentially expression only at the protein level or only differentially expressed at the phosphoprotein level and their related signalling pathways are crucial in regulating puberty in goats. These differentially expressed proteins and phosphorylated proteins may constitute the proteomic backgrounds between the two different stages.

show abstract

“…Post-translational modification through phosphorylation is critical in regulating virus infection, as virus-encoded proteins are often involved in this process [11]. For instance, the phosphorylation/dephosphorylation of the replicase cofactor p33 to Cucumber necrosis virus (CMV) regulates virus replication and transcription [12].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the TGBp1 of Barley stripe mosaic virus is phosphorylated by CK2 protein kinase and regulates virus cell-to-cell and systemic movement in both Nicotiana benthamiana and barley [16]. Phosphorylation of PVX TGBp1 at Ser-165 in N. tabacum by CK2-like kinase also regulates viral infectivity and pathogenicity [11,17].…”

Section: Introductionmentioning

confidence: 99%

The phosphorylation of the movement protein TGBp1 regulates the accumulation of the Bamboo mosaic virus

Wu,

Chen,

Hou

et al. 2024

Journal of General Virology

View full text Add to dashboard Cite

Phosphorylation and dephosphorylation of viral movement proteins plays a crucial role in regulating virus movement. Our study focused on investigating the movement protein TGBp1 of Bamboo mosaic virus (BaMV), which is a single-stranded positive-sense RNA virus. Specifically, we examined four potential phosphorylation sites (S15, S18, T58, and S247) within the TGBp1 protein. To study the impact of phosphorylation, we introduced amino acid substitutions at the selected sites. Alanine substitutions were used to prevent phosphorylation, while aspartate substitutions were employed to mimic phosphorylation. Our findings suggest that mimicking phosphorylation at S15, S18 and T58 of TGBp1 might be linked to silencing suppressor activities. The phosphorylated form at these sites exhibits a loss of silencing suppressor activity, leading to reduced viral accumulation in the inoculated leaves. Furthermore, mimicking phosphorylation at residues S15 and S18 could diminish viral accumulation at the single-cell level, while doing so at residue T58 could influence virus movement. However, mimicking phosphorylation at residue S247 does not appear to be relevant to both functions of TGBp1. Overall, our study provides insights into the functional significance of specific phosphorylation sites in BaMV TGBp1, illuminating the regulatory mechanisms involved in virus movement and silencing suppression.

show abstract

Phosphorylation of plant virus proteins: Analysis methods and biological functions

Cited by 5 publications

References 153 publications

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

Integrative proteomic and phosphoproteomic analysis in the female goat hypothalamus to study the onset of puberty

The phosphorylation of the movement protein TGBp1 regulates the accumulation of the Bamboo mosaic virus

Contact Info

Product

Resources

About