“…To address the question of the intracellular effect of Gemin2 phosphorylation, cells which inducibly overexpress GFP, GFP-SMN wt, GFP-Gemin2 wt, GFP-Gemin2 A81, GFP-Gemin2 A166, GFP-Gemin2 D81, and GFP-Gemin2 D166 were generated (Figure 4A,B). Size exclusion chromatography pointed out that Gemin2 is distributed in a higher molecular complex as well as in the distinct molecular weight range from 158 kDa to 100 kDa, the same size range as the 6S complex [36]. This suggests that the 6S complex, consisting of pICln and the Sm proteins D1, D2, E, F, and G, might be a kinetic trap involved in the transfer of Sm proteins onto the SMN complex to keep snRNP biogenesis ongoing [24].…”