Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells

Gui, Jinshan; Luo, Laifu; Zhong, Yu; Sun, Jing; Umezawa, Toshiaki; Li, Laigeng

doi:10.1016/j.molp.2019.05.008

Cited by 83 publications

(67 citation statements)

References 51 publications

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“…The gene expression data were normalised using the Populus actin2 gene ( Potri.001G309500 ). Primer pairs used for real‐time PCR are as described previously (Gui et al , ).…”

Section: Methodsmentioning

confidence: 99%

“…Lignin biosynthesis‐related transcription factor 1 (LTF1), belonging to the R2R3‐MYB subfamily, binds to the promoter of lignin biosynthesis genes to represses lignin biosynthesis in Populus (Gui et al , ). Several members in this subfamily across various plant species are reported to play a role in regulation of lignin biosynthesis (Zhao & Dixon, ; Rao & Dixon, ; Rao et al , ).…”

Section: Introductionmentioning

confidence: 99%

“…LTF1 shares a high degree of sequence homology with AtMYB4 in Arabidopsis, which is involved in regulation of the phenylproponoid metabolism pathway (Jin et al , ). In Populus , LTF1 can be phosphorylated in response to external stimuli, however the phosphorylation sites are not conserved in AtMYB4 (Gui et al , ). The phosphorylated LTF1 is degraded which leads to the release of its suppression on lignin biosynthesis and thus the phosphorylation‐null LTF1 can convey a persistent suppression of lignin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus

et al. 2020

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Lignin is a major component of cell wall biomass and decisively affects biomass utilisation. Engineering of lignin biosynthesis is extensively studied, while lignin modification often causes growth defects.We developed a strategy for cell-type-specific modification of lignin to achieve improvements in cell wall property without growth penalty. We targeted a lignin-related transcription factor, LTF1, for modification of lignin biosynthesis. LTF1 can be engineered to a nonphosphorylation form which is introduced into Populus under the control of either a vessel-specific or fibre-specific promoter.The transgenics with lignin suppression in vessels showed severe dwarfism and thin-walled vessels, while the transgenics with lignin suppression in fibres displayed vigorous growth with normal vessels under phytotron, glasshouse and field conditions. In-depth lignin structural analyses revealed that such cell-type-specific downregulation of lignin biosynthesis led to the alteration of overall lignin composition in xylem tissues reflecting the population of distinctive lignin polymers produced in vessel and fibre cells.This study demonstrates that fibre-specific suppression of lignin biosynthesis resulted in the improvement of wood biomass quality and saccharification efficiency and presents an effective strategy to precisely regulate lignin biosynthesis with desired growth performance.

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“…The gene expression data were normalised using the Populus actin2 gene ( Potri.001G309500 ). Primer pairs used for real‐time PCR are as described previously (Gui et al , ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus

et al. 2020

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“…Alternatively, phosphorylation may also constitute a signal for protein degradation through proteasome activity. By screening TFs binding to the poplar 4CL promoter, Gui and colleagues identified a lignin biosynthesis-associated factor, LTF1, that represses several genes from this pathway (PAL2, C4H1, C3H2, 4CL1, CAld5H, COMT2, and CCoAOMT1) and decreases lignin content in overexpressing lines [107]. After phosphorylation, LTF1 is targeted to the proteasome, thereby derepressing lignin deposition.…”

Section: Switching On/off Enzymatic Activity With Phosphorylationmentioning

confidence: 99%

“…After phosphorylation, LTF1 is targeted to the proteasome, thereby derepressing lignin deposition. This post-transcriptional regulation seems to be mediated by environmental factors such as wounding, and may trigger rapid lignification as a part of plant response to external stimulation [107].…”

Section: Switching On/off Enzymatic Activity With Phosphorylationmentioning

confidence: 99%

A Molecular Blueprint of Lignin Repression

Behr¹,

Guerriero²,

Grima‐Pettenati³

et al. 2019

Trends in Plant Science

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Although lignin is essential to ensure the correct growth and development of land plants, it may be an obstacle to the production of lignocellulosics-based biofuels, and reduces the nutritional quality of crops used for human consumption or livestock feed. The need to tailor the lignocellulosic biomass for more efficient biofuel production or for improved plant digestibility has fostered considerable advances in our understanding of the lignin biosynthetic pathway and its regulation. Most of the described regulators are transcriptional activators of lignin biosynthesis, but considerably less attention has been devoted to the repressors of this pathway. We provide a comprehensive overview of the molecular factors that negatively impact on the lignification process at both the transcriptional and post-transcriptional levels. Challenging LigninLignin is a major cell wall component that fulfills fundamental functions in plant development as well as in defense against pests and pathogens. This heteropolymer impregnates the compound middle lamella and the secondary cell walls (SCWs) of cells genetically programmed to be lignified, such as xylem tracheary elements as well as xylem and phloem fibers. Lignin biosynthesis spans the phenylpropanoid and the monolignol pathways, from phenylalanine to p-coumaryl, coniferyl, and sinapyl alcohols. Monolignol homeostasis is regulated through a balance between the molecular factors that antagonistically regulate their biosynthesis. After monolignols are excreted into the apoplast, they are oxidized by the phenol-oxidoreductase laccases and/or by class III peroxidases, and are polymerized by radical coupling. A complex regulatory network involving phytohormones, transcription factors (TFs), and post-transcriptional events guide SCW deposition and lignification [1]. This network prevents lignin deposition in tissues undergoing active division or elongation such as apical meristems, vascular cambium, and immature xylem cells, as well as in non-lignified tissues such as stem pith and flax or hemp bast fibers that harbor gelatinous walls under normal developmental conditions. Similarly, this network negatively regulates lignification in xylem tissue formed in response to mechanical stresses such as, for instance, poplar tension wood that harbors hypolignified gelatinous walls.

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Mitigating biomass recalcitrance for plant‐based bioenergy production

Ninkuu,

Liu,

Zhou

et al. 2023

Modern Agriculture

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The emission of greenhouse gases, particularly carbon dioxide, predominantly from fossil fuel combustion has received critical warnings several times as their levels exceed the tolerable limits in view of global warming. This calls for a paradigm shift from a fossil fuel‐based source to a less hazardous bioenergy source. Plant feedstock is an attractive source of raw materials for bioenergy production; however, chemical or enzymatic digestion of the feedstock is expensive owing to the supramolecular lignocellulosic barrier, indicating the need for better alternatives. Several attempts have been made towards reducing the biomass recalcitrance of straw using genetic transformations. We present a review highlighting potential plant candidates for bioenergy production, the lignocellulose composition of the feedstock, how the composition can impede enzymatic degradation, the regulation of lignocellulose polymer biosynthesis, and the influence of genetic transformation on biomass saccharification. Moreover, the review also discusses conflicting research interests in biomass recalcitrance and suggests a common ground. The review findings suggest that bioenergy production from crop straws will drastically reduce over‐dependence on fossil fuels and consequently pollution levels.

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Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells

Cited by 83 publications

References 51 publications

Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus

Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus

A Molecular Blueprint of Lignin Repression

Mitigating biomass recalcitrance for plant‐based bioenergy production

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