Phosphorylation of HspB1 regulates its mechanosensitive molecular chaperone interaction with native filamin C

Abstract

Small heat-shock proteins (sHsps; HspBs) are molecular chaperones involved in the cellular stress response and a range of basal functions. Despite a multitude of targets, sHsp interactions are not well understood due their heterogeneous structures and weak binding affinities. The most widely expressed human sHsp, HspB1, is prevalent in striated muscle, where the actin cross-linker filamin C (FLNC, γ-filamin, ABP-L) is a putative binding partner. Musculoskeletal HspB1 is phosphorylated in response to a variety of cues, including mechanical stress, which promotes oligomer disassembly and association with myoarchitectural elements. Here, we report the up-regulation and interaction of both proteins in the hearts of a mouse model of heart failure, with HspB1 being phosphorylated and FLNC increasingly associated with the sarcomeric Z-disc. We used a combination of structural approaches to reveal that phosphorylation of HspB1 results in increased availability of the residues surrounding the phosphosite, facilitating their interaction with folded FLNC domains equivalent to a force-sensing region in the paralog filamin A. By employing native mass spectrometry, we show that domains 18 to 21 of FLNC are extensible under conditions mimicking force, with phosphorylated HspB1 stabilising an intermediate from further unfolding. These findings report on conformations accessible during the cycles of mechanical extension central to filamin function, and are consistent with an interaction between the chaperone and a native target that is strengthened upon the application of force. This may represent a new mode of molecular chaperone activity, allowing HspB1 to protect FLNC from over-extension during mechanical stress.

Time-resolved proximity proteomics uncovers a membrane tension-sensitive caveolin-1 interactome at the rear of migrating cells

“…Among these proteins were Rho-associated protein kinase 1 (ROCK1) and the heat shock protein HSPB1. Rho/ROCK1 signaling was previously shown to be required for caveolae localisation to the rear of migrating cells (39) and HSPB1 is a stress-regulated actin binding protein implicated in mechanotransduction (58,59). Pathway enrichment analysis suggested a functional link between Cav1, ROCK1, and HSPB1 (Figure 3J).…”

“…The copyright holder for this this version posted December 14, 2022. ; https://doi.org/10.1101/2022.12.13.520222 doi: bioRxiv preprint and HSPB1 is a stress-regulated actin binding protein implicated in mechanotransduction (58,59). Pathway enrichment analysis suggested a functional link between Cav1, ROCK1, and HSPB1 (Figure 3J).…”