2008
DOI: 10.1128/mcb.02117-07
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Phosphorylation of Fibroblast Growth Factor (FGF) Receptor 1 at Ser777 by p38 Mitogen-Activated Protein Kinase Regulates Translocation of Exogenous FGF1 to the Cytosol and Nucleus

Abstract: Exogenous fibroblast growth factor 1 (FGF1) signals through activation of transmembrane FGF receptors (FGFRs) but may also regulate cellular processes after translocation to the cytosol and nucleus of target cells. Translocation of FGF1 occurs across the limiting membrane of intracellular vesicles and is a regulated process that depends on the C-terminal tail of the FGFR. Here, we report that translocation of FGF1 requires activity of the ␣ isoform of p38 mitogen-activated protein kinase (MAPK). FGF1 transloca… Show more

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Cited by 54 publications
(64 citation statements)
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References 66 publications
(106 reference statements)
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“…In order to study the internalization of the fusion protein in a variety of cell lines, we performed digitonin-based cell fractionation as reported previously (Sorensen et al 2008). HeLa cervical carcinoma cells treated with C2IN-Stv13 and the binding/translocation component C2IIa displayed a time-dependent accumulation of C2IN-Stv13 in the cytosol with a maximum of protein translocated after 4 h (Fig.…”
Section: Uptake Of C2in-stv13 Into the Cytosol Of Cancer Cellsmentioning
confidence: 99%
See 1 more Smart Citation
“…In order to study the internalization of the fusion protein in a variety of cell lines, we performed digitonin-based cell fractionation as reported previously (Sorensen et al 2008). HeLa cervical carcinoma cells treated with C2IN-Stv13 and the binding/translocation component C2IIa displayed a time-dependent accumulation of C2IN-Stv13 in the cytosol with a maximum of protein translocated after 4 h (Fig.…”
Section: Uptake Of C2in-stv13 Into the Cytosol Of Cancer Cellsmentioning
confidence: 99%
“…To study the cytosolic translocation of the C2IN-Stv13 fusion protein, digitonin-based cell fractionation was used according to Sørensen and co-workers (Sorensen et al 2008). Briefly, cells grown in 12-well plates were preincubated in serum-free medium for 15 min and then supplemented with a combination of C2IN-Stv13 (2 μg/ml) and C2IIa (4 μg/ml).…”
Section: Cell Fractionation Using Digitoninmentioning
confidence: 99%
“…Two siRNA sequences, GAACGUUGUUUCCUGGUACTT (␣1) and GAUGCUCGUUUUGGACUCAG (␣2), targeting mouse p38␣ mRNA were the same as previously published (28) and HPLC purified (MWG Biotech) with 2-nt 3Ј terminal dTdT overhangs at each strand. A negative control siRNA was obtained from Dharmacon.…”
Section: Transfection Of P38␣ Small Interfering Rna (Sirna)mentioning
confidence: 99%
“…4C). Since Ser/Thr phosphorylation of several growth factor receptors has been associated with tyrosine kinase inhibition (5,7,11,16,34,35,44,55), these results raise the possibility that PP2A promotes TrkA activity by dephosphorylating inhibitory Ser/Thr phosphorylation sites.…”
Section: Pp2a Sustains Ngf-dependent Trka Autophosphorylationmentioning
confidence: 84%
“…1) suggests that the Ser/Thr phosphatase opposes a delayed, Trk-specific desensitization mechanism. Ser/Thr phosphorylation of several receptor tyrosine kinases has been re- (5,7,11,16,34,35,44,55). In the case of the hepatocyte growth factor receptor cMET, PP2A dephosphorylates an inhibitory protein kinase C phosphorylation site in the tyrosine kinase domain, thereby keeping cMET active (20).…”
Section: Discussionmentioning
confidence: 99%