2003
DOI: 10.1093/intimm/dxg116
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Phosphorylation of class II transactivator regulates its interaction ability and transactivation function

Abstract: The MHC class II transactivator (CIITA) plays a central role in adaptive immune responses by controlling the expression of MHC class II genes. CIITA binds DNA-binding proteins and co-activator proteins to form an enhanceosome complex necessary for MHC class II gene expression. Here we demonstrate that CIITA interactions depend upon the phosphorylation status of CIITA. Hyper-phosphorylated CIITA interacts with co-activator p300, RFX5 and CIITA itself, which in turn results in induction of MHC class II promoter … Show more

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Cited by 39 publications
(31 citation statements)
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“…Interestingly, the Neu RTK activates the mitogen activated kinase signaling cascade, which in turn can increase the transcriptional activity of CIITA. 39,40 Variable levels of expression of the transduced genes and/or the extinction of MHC class II on tumor cells might also explain some of the differences in results between these studies.…”
Section: Discussionmentioning
confidence: 97%
“…Interestingly, the Neu RTK activates the mitogen activated kinase signaling cascade, which in turn can increase the transcriptional activity of CIITA. 39,40 Variable levels of expression of the transduced genes and/or the extinction of MHC class II on tumor cells might also explain some of the differences in results between these studies.…”
Section: Discussionmentioning
confidence: 97%
“…To generate stable cell lines expressing WT or DN PKCδ, RAW264.7 cells were transfected with WT, DN PKCδ or the empty vector, and selected with G418. The 1.1 kb pIV-driven luciferase plasmid and MHC class II Eα driven luciferase reporter plasmid were described previously (Sisk et al, 2003;Yao et al, 2006). The IRF-1 promoter construct that contains the GAS and NF-κB site (0.7kb) was kindly provided by Dr. Richard Pine (Public Health Research Institute, New York, NY) (Pine, 1997).…”
Section: Plasmid and Transfectionsmentioning
confidence: 99%
“…Two days later, the cells were treated with human IFN-γ for 12hrs. Cell lysates were prepared to measure the luciferase activity as described (Sisk et al, 2003). Luciferase activity was normalized with CMV promoter driven β-galactosidase expression.…”
Section: Plasmid and Transfectionsmentioning
confidence: 99%
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“…Although the CIITA gene is subject to both constitutive and inducible expression, the CIITA protein is also targeted by posttranslational modifications that alter its activity. Phosphorylation within the PST domain of CIITA by the kinases protein kinase A, protein kinase C, GSK3, CK1, and ERK1/2 selectively enhance or inhibit target gene expression (34)(35)(36)(37)(38)(39). Acetylation of CIITA by p300/CBP and pCAF increases its transactivation ability (40,41), whereas deacetylation by HDAC1 leads to its proteasomal degradation (42).…”
mentioning
confidence: 99%