Phosphorylation by Casein Kinase-2 and Reversible Alteration of Thiol Groups: Mechanisms of Control of Ornithine Decarboxylase?

Flamigni, Flavio; Meggio, Flavio; Marmiroli, Sandra; Guarnieri, Carlo; Pinna, Lorenzo A.; Caldarera, Claudio Marcello

doi:10.1007/978-1-4684-5637-0_5

Cited by 3 publications

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“…ODC expression has been suggested to be regulated at the translational level in mouse testis (Alcivar et al, 1989), Xenopus laevis oocytes (Bassez et al, 1990), Ehrlich ascites tumor cells (Wallon et al, 1990), and murine 3T3 fibroblasts (Levine et al, 1990). There are also reports that ODC activity is post-translationally inactivated in polyaminetreated cultured cells (Flamigni et al, 1988) and regenerating liver of ethanol-fed rats (Diehl et al, 1990) and activated in murine colony-stimulatingfactor-dependent cells (Farrar and Harel-Bellan, 1989) and injured gastric mucosa ofaged rats (Edgerton et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

Effects of Sialagogues on Ornithine Decarboxylase Induction and Proto-oncogene Expression in Murine Parotid Gland

et al. 1992

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The mechanism of a sialagogue-induced increase in ornithine decarboxylase (ODC) activity and the expressions of proto-oncogenes in murine parotid gland were investigated by use of isoproterenol (IPR), carbachol (CC), and methoxamine (MTX). The results were as follows: (1) The three sialagogues had similar effects on the parotid in vivo (mouse parotid after a single injection of IPR) and/or in vitro (rat parotid explants cultured on siliconized lens paper floating on 199 medium containing IPR, CC, or MTX), the order of their effectiveness being IPR > CC > MTX. (2) Northern/dot and Western blot analyses revealed that the sialagogues elevated the steady-state levels of ODC mRNA and ODC protein to maxima at two h and six h, respectively, after stimulation. The increases were roughly proportional to those in ODC activity, suggesting that sialagogue-dependent enzyme induction is regulated at the transcriptional level. (3) The mRNAs of four of nine proto-oncogenes examined showed sialagogue-dependent increases to maxima at 30 min (c-fos) or 60 min (c-jun, c-myc, and c-src) after the beginning of stimulation. These increases were all transient, with the levels returning to the control values (without sialagogue) within 60 min. (4) The IPR-dependent elevations of ODC activity and the mRNAs of ODC, c-fos, and c-jun were inhibited by monensin, but not by polymyxin B. On the other hand, the CC-dependent increases in these parameters were inhibited by polymyxin B but not by monensin.(ABSTRACT TRUNCATED AT 250 WORDS)

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