Phosphorus storage in<i>Chlorella vulgaris</i>(Trebouxiophyceae, Chlorophyta) cells and its dependence on phosphate supply

Eixler, Sebastian; Karsten, Ulf; Selig, Uwe

doi:10.2216/04-79.1

Cited by 124 publications

(59 citation statements)

References 36 publications

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“…A similar phenomenon has been observed in other microalgae (Markou et al 2012c;Wu et al 2012). This may be attributed to the storage of excessive phosphorus as polyphosphate that can serve as an internal resource for cell survival when external phosphorus is limiting (Eixler et al 2006;Nishikawa et al 2006). Our results show that phosphorus was completely removed from the medium within 1 day (Fig.…”

Section: Discussionmentioning

confidence: 56%

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Yao

Cao

et al. 2013

Appl Microbiol Biotechnol

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Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell densities (ICD) of 1.5, 3.0, 6.0, and 9.0×10 6 cells mL were all obtained in the culture with the ICD of 3.0× 10 6 cells mL −1 . Appropriate ICD could be used to regulate the intracellular phosphorus concentration and maintain adequate photosynthetic activity to achieve the highest starch productivity, along with biomass and starch concentration. The recovery of phosphorus-deprived T. subcordiformis in medium containing 0.5, 1.0, or 6.0 mM KH 2 PO 4 was also tested. Cell growth and starch accumulation ability could be recovered completely. A phosphorus pool in T. subcordiformis was shown to manipulate its metabolic activity under different environmental phosphorus availability. Though lower starch productivity and starch content were achieved under phosphorus deprivation compared with nitrogen-or sulfur-deprived conditions, the higher biomass and starch concentration make T. subcordiformis a good candidate for biomass and starch production under extracellular phosphorus deprivation.

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Section: Discussionmentioning

confidence: 56%

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Yao

Cao

et al. 2013

Appl Microbiol Biotechnol

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“…All experimental flasks were maintained during 24 h at the same physical conditions of aeration and light environment as the preculture. Luxury consumption can be generally detected in a few hours after onset the perturbation (Eixler et al, 2006). Preculture was not axenic, but it was unialgal and free of protozoan and fungal contaminants; sterile technique was used throughout.…”

Section: Methodsmentioning

confidence: 99%

“…Sterner and Elser (2002) hypothesized that the N:P content of producers would closely match the N:P ratio supplied to ecosystems because of the ability of autotrophs to store nutrients through luxury consumption, which refers to 'increases in organismal nutrients over and above what is immediately required for growth' or the minimal elemental requirements. Generally, luxury uptake of nutrients can be detected in a few hours after onset a nutrient pulsed perturbation (e.g., see Eixler et al 2006). In this short-term, changes in cell elemental composition are involved in the acclimation process (Giordano, 2013).…”

Section: Introductionmentioning

confidence: 99%

Strict stoichiometric homeostasis of Cryptomonas pyrenoidifera (Cryptophyceae) in relation to N:P supply ratios

2016

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A common freshwater cryptophyte, Cryptomonas pyrenoidifera, was cultivated in batch-cultures to analyze intraspecific variation in elemental stoichiometry along a broad gradient of pulsed phosphorus (P) enrichment during the early acclimation period and to determine the immediate homeostatic capacity of the nitrogen-to-phosphorus (N:P) ratio of this alga when nutrients are at saturating levels. Experimental results revealed that nitrogen (N) and P cell quotas significantly increased with increasing P concentration. However, despite the wide range of N:P ratios in the medium, C. pyrenoidifera N:P ratios were highly stable at higher P-level treatments, indicating a highly conservative behavior and suggesting strict elemental homeostasis when nutrients are at saturating levels. The strictly homeostatic N:P ratio appears to be attributable to their high potential for a fast luxury consumption of both N and P after a brief and intense episode of increased resource availability and to physiological limits on their nutrient storage capacity. Most importantly, the N:P biomass ratio at nutrient saturating levels converged around 11:1, which was the observed ratio of maximum internal cell quotas for N and P (i.e., Q max , N :Q max , P ) under the prevailing experimental conditions. This value is particularly informative for C. pyrenoidifera because it represents cell storage quotients and may be a taxon-specific evolutionary optimum, providing a reference point to infer the grade of nutrient-limitation. The experimental data give ranges of variation in C. pyrenoidifera elemental composition permitting, among others, proper parameterization of cryptophyte stoichiometry models.

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“…그리고 이러한 현상을 "Luxury uptake" 라고 부른다 (Eixler et al, 2006). microalgae-A review of technologies for production, prodcessing, and extraction of biofuels and co-products" Renewable and Sustainable Energy Reviews, Vol.…”

Section: 서 론mentioning

confidence: 99%

Effects of phosphorus concentration and nitrogen sources on photoautotrophic microalgae Scenedesmus dimorphus applied wastewater treatment

Kim¹,

Cho²,

Park³

et al. 2013

Journal of Korean Society of Water and Wastewater

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: A result of estimating the effects of initial phosphorus concentration and nitrogen sources on removal of nitrogen and phosphorus in wastewater treatment using Scenedesmus dimorphus shows that there was no difference in microalgae growth amount and nitrogen removal amount by phosphorus concentration(5 ~ 60 mg/L). On the other hand, as initial phosphorus concentration increased, phosphorus removal amount was increased by luxury uptake of microalgae. Scenedesmus dimorphus preferred to ammonia when ammonia(40 mg/L) and nitrate(40 mg/L) are simultaneously presented and nitrogen removal rate of ammonia was high at approximately 20 mg/L/day.

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Phosphorus storage inChlorella vulgaris(Trebouxiophyceae, Chlorophyta) cells and its dependence on phosphate supply

Cited by 124 publications

References 36 publications

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Strict stoichiometric homeostasis of Cryptomonas pyrenoidifera (Cryptophyceae) in relation to N:P supply ratios

Effects of phosphorus concentration and nitrogen sources on photoautotrophic microalgae Scenedesmus dimorphus applied wastewater treatment

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