Phosphorus starvation and luxury uptake in green microalgae revisited

Solovchenko, Alexei; Khozin‐Goldberg, Inna; Selyakh, Irina; Semenova, Larisa; Ismagulova, T. T.; Lukyanov, Alexander; Mamedov, Ilgar Z.; Vinogradova, Elizaveta; Карпова, О. В.; Konyukhov, I. V.; Vasilieva, S. G.; Mojzeš, Peter; Dijkema, C.; Vecherskaya, M; Zvyagin, Ivan V.; Nedbal, Ladislav; Gorelova, Olga

doi:10.1016/j.algal.2019.101651

Cited by 77 publications

(63 citation statements)

References 59 publications

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“…On the other hand, the excessive formation of short-chain PolyP must be avoided, since the latter can exert a toxic effect [ 71 , 72 ]. This speculation is supported by the transient increase in PolyP observed in this work and in other experiments with the refeeding of P-starved photosynthetic cells [ 59 ].…”

Section: Discussionsupporting

confidence: 85%

“…The analysis of the available literature on LPU in cyanobacteria and eukaryotic microalgae (e.g., [ 6 , 7 , 9 , 59 ]) reveals a lot of inconsistencies pointed out, e.g., in [ 21 ]. Still, it shows that the observed characteristics of LPU strongly depend on the cultivation conditions and P nutrition prehistory of the culture.…”

Section: Discussionmentioning

confidence: 99%

“…These pools differ dramatically by their size and flexibility of response to P availability in the medium [ 9 ], posing challenges to the reliable estimation of the actual cell P quota size. Therefore, to make our experiments on LUP as deterministic as possible, we used the cells with the P quota depleted by depriving them of P until the cell division is ceased [ 59 ]. This approach also allows us to reconstruct the events taking place in natural cyanobacterial populations upon the abrupt increase of P availability, e.g., as a result of upwelling [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…The latter is important, since the P-rich biomass gradually decomposed by soil microbial phosphatases acts as a slow-release P fertilizer [ 80 ]. As was previously shown for eukaryotic microalgae, the P-starved cells best capable of P i uptake are potentially suitable for the post-treatment of wastewater [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

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Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Solovchenko

Gorelova

Карпова

et al. 2020

Cells

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To cope with fluctuating phosphorus (P) availability, cyanobacteria developed diverse acclimations, including luxury P uptake (LPU)—taking up P in excess of the current metabolic demand. LPU is underexplored, despite its importance for nutrient-driven rearrangements in aquatic ecosystems. We studied the LPU after the refeeding of P-deprived cyanobacterium Nostoc sp. PCC 7118 with inorganic phosphate (Pi), including the kinetics of Pi uptake, turnover of polyphosphate, cell ultrastructure, and gene expression. The P-deprived cells deployed acclimations to P shortage (reduction of photosynthetic apparatus and mobilization of cell P reserves). The P-starved cells capable of LPU exhibited a biphasic kinetic of the Pi uptake and polyphosphate formation. The first (fast) phase (1–2 h after Pi refeeding) occurred independently of light and temperature. It was accompanied by a transient accumulation of polyphosphate, still upregulated genes encoding high-affinity Pi transporters, and an ATP-dependent polyphosphate kinase. During the second (slow) phase, recovery from P starvation was accompanied by the downregulation of these genes. Our study revealed no specific acclimation to ample P conditions in Nostoc sp. PCC 7118. We conclude that the observed LPU phenomenon does not likely result from the activation of a mechanism specific for ample P conditions. On the contrary, it stems from slow disengagement of the low-P responses after the abrupt transition from low-P to ample P conditions.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Solovchenko

Gorelova

Карпова

et al. 2020

Cells

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“…The ultrastructural manifestations of chloroplast degradation, a reduction in cytoplasmic ribosome abundance, and the formation of autophagic vacuoles in the course of lipid droplet (LD) formation were observed in microalgae under various stresses (Zhao et al, 2014;Shebanova et al, 2017;Tsai et al, 2017;Gorelova et al, 2018). Phosphorous (P) deprivation is mitigated by an array of mechanisms, which include membrane lipid remodeling in favor of non-phosphorous lipids, reallocation of cellular C resources, P scavenging from endogenous reserves, and autophagy (Feng et al, 2015;Cañavate et al, 2017;Alipanah et al, 2018;Solovchenko et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich Microalga Lobosphaera incisa Under Nitrogen and Phosphorous Deprivation

et al. 2020

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The green microalga Lobosphaera incisa accumulates triacylglycerols (TAGs) with exceptionally high levels of long-chain polyunsaturated fatty acid (LC-PUFA) arachidonic acid (ARA) under nitrogen (N) deprivation. Phosphorous (P) deprivation induces milder changes in fatty acid composition, cell ultrastructure, and growth performance. We hypothesized that the resource-demanding biosynthesis and sequestration of ARA-rich TAG in lipid droplets (LDs) are associated with the enhancement of catabolic processes, including membrane lipid turnover and autophagic activity. Although this work focuses mainly on N deprivation, a comparative analysis of N and P deprivation responses is included. The results of lipidomic profiling showed a differential impact of N and P deprivation on the reorganization of glycerolipids. The formation of TAG under N deprivation was associated with the enhanced breakdown of chloroplast glycerolipids and the formation of lyso-lipids. N-deprived cells displayed a profound reorganization of cell ultrastructure, including internalization of cellular material into autophagic vacuoles, concomitant with the formation of LDs, while P-deprived cells showed better cellular ultrastructural integrity. The expression of the hallmark autophagy protein ATG8 and the major lipid droplet protein (MLDP) genes were coordinately upregulated, but to different extents under either N or P deprivation. The expression of the Δ5-desaturase gene, involved in the final step of ARA biosynthesis, was coordinated with ATG8 and MLDP, exclusively under N deprivation. Concanamycin A, the inhibitor of vacuolar proteolysis and autophagic flux, suppressed growth and enhanced levels of ATG8 and TAG in N-replete cells. The proportions of ARA in TAG decreased with a concomitant increase in oleic acid under both N-replete and N-deprived conditions. The photosynthetic apparatus’s recovery from N deprivation was impaired in the presence of the inhibitor, along with the delayed LD degradation. The GFP-ATG8 processing assay showed the release of free GFP in N-replete and N-deprived cells, supporting the existence of autophagic flux. This study provides the first insight into the homeostatic role of autophagy in L. incisa and points to a possible metabolic link between autophagy and ARA-rich TAG biosynthesis.

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Impact of Native Biostimulants/Biofertilizers and Their Synergistic Interactions On the Agro-physiological and Biochemical Responses of Date Palm Seedlings

et al. 2022

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Phosphorus starvation and luxury uptake in green microalgae revisited

Cited by 77 publications

References 59 publications

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Phosphorus Feast and Famine in Cyanobacteria: Is Luxury Uptake of the Nutrient Just a Consequence of Acclimation to Its Shortage?

Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich Microalga Lobosphaera incisa Under Nitrogen and Phosphorous Deprivation

Impact of Native Biostimulants/Biofertilizers and Their Synergistic Interactions On the Agro-physiological and Biochemical Responses of Date Palm Seedlings

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