Phosphorus limitation in a Ferralsol: Impact on microbial activity and cell internal P pools

Ehlers, Knut; Bakken, Lars R.; Frostegård, Åsa; Frossard, Emmanuel; Bünemann, Else K.

doi:10.1016/j.soilbio.2009.11.025

Cited by 108 publications

(57 citation statements)

References 64 publications

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“…A recent study by Esberg et al (2010) showed correlation between microbial respiration and changes in NaOHextractable P and suggested that microbial access to this fraction was greater. Likewise, Bü nemann et al (2004) and Ehlers et al (2010) have suggested greater access to fixed pools of inorganic P may occur in Ferrosol soils in response to growth enhancement of microorganisms resulting from addition of C substrates under laboratory incubations. Further work to quantify the relative ability of microorganisms to access different fractions of soil P, as compared with plants, is warranted.…”

Section: Solubilization Of Inorganic Pmentioning

confidence: 98%

“…More recent studies have shown that a severalfold increase in microbial P in response to added C (and nitrogen [N]) is associated with a significant decline in soil solution orthophosphate. This occurs in soils that are either P amended, or without added P and in soils that are considered to be P limited (Oehl et al, 2001;Bü nemann et al, 2004;Ehlers et al, 2010). It is evident therefore that microorganisms effectively compete with plants for available orthophosphate from soil solution and also represent a significant pool of immobilized P that is temporarily unavailable to plants (Fig.…”

Section: Accumulation and Turnover Of Microbial Biomass Pmentioning

confidence: 99%

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Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

2011

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Section: Solubilization Of Inorganic Pmentioning

confidence: 98%

Section: Accumulation and Turnover Of Microbial Biomass Pmentioning

confidence: 99%

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

2011

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“…This was the case in combination with all crops tested. It is well documented that the application of organic materials enhances the microbial biomass and microbial activity of soils (Ehlers et al, 2010;Krey et al, 2013;Requejo and Eichler-Löbermann, 2014). Nonetheless, this depends on the material applied.…”

Section: Effect Of the Digestates On Plant And Soil Parametersmentioning

confidence: 99%

Phosphorus distribution and availability in untreated and mechanically separated biogas digestates

Bachmann

Uptmoor

Eichler‐Löbermann

2016

Sci. agric. (Piracicaba, Braz.)

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Biogas digestates contain valuable nutrients but also have high water contents. Digestates were sampled from two different biogas facilities before and after solid-liquid separation and were analyzed with regard to their composition and phosphorus (P) fractions. Additionally, to investigate the P fertilizer effects of these digestates in comparison with undigested slurry or TripleSuper-P (TSP), they were applied in a pot experiment (6 kg soil per pot) in an amount corresponding to 200 mg P per pot in combination with various crops (amaranth, maize, maize + beans mixed cropping, sorghum). A separation of digestates resulted in higher P concentrations of the solid fraction in comparison with the liquid fraction. The proportion of the readily soluble P fractions (H 2 O-P, NaHCO 3 -P) to the total P was higher than 70 % in all digestates. The digestates increased P uptake of the tested crops and concentrations of bioavailable P in the soil to the same extent as highly soluble TSP. Activities of soil enzymes were lower after application of the digestates in comparison to unfermented slurry. The fertilizer management of digestates can be improved by a solid-liquid separation since the solid fraction showed a relatively high concentration of P resulting in a reduction in application doses required to meet the P demands of crops.

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“…Plants and soil microorganisms take up phosphorus (P) as inorganic phosphate (P i ) from the soil solution and low P i concentrations can limit biological growth and crop production in many ecosystems (Ehlers et al, 2010;Richardson et al, 2011). Under P-limiting conditions, some plants and mi-C. von Sperber et al: The oxygen isotope composition of phosphate released from phytic acid croorganisms can exude phytases, which catalyze the hydrolysis of phosphomonoester bonds in IP 6 leading to the release of inorganic phosphate (P i ) (Hayes et al, 1999;Richardson et al, 2000Richardson et al, , 2001Lung and Lim, 2006;Li et al, 1997a, b).…”

Section: Introductionmentioning

confidence: 99%

The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and <i>Aspergillus niger</i> phytase

et al. 2015

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Abstract. Phosphorus (P) is an essential nutrient for living organisms. Under P-limiting conditions plants and microorganisms can exude extracellular phosphatases that release inorganic phosphate (P i ) from organic phosphorus compounds (P org ). Phytic acid (myo-inositol hexakisphosphate, IP 6 ) is an important form of P org in many soils. The enzymatic hydrolysis of IP 6 by phytase yields available P i and less phosphorylated inositol derivates as products. The hydrolysis of organic P compounds by phosphatases leaves an isotopic imprint on the oxygen isotope composition (δ 18 O) of released P i , which might be used to trace P in the environment. This study aims at determining the effect of phytase on the oxygen isotope composition of released P i . For this purpose, enzymatic assays with histidine acid phytases from wheat and Aspergillus niger were prepared using IP 6 , adenosine 5 -monophosphate (AMP) and glycerophosphate (GPO 4 ) as substrates. For a comparison to the δ 18 O of P i released by other extracellular enzymes, enzymatic assays with acid phosphatases from potato and wheat germ with IP 6 as a substrate were prepared. During the hydrolysis of IP 6 by phytase, four of the six P i were released, and one oxygen atom from water was incorporated into each P i . This incorporation of oxygen from water into P i was subject to an apparent inverse isotopic fractionation (ε ∼ 6 to 10 ‰), which was similar to that imparted by acid phosphatase from potato during the hydrolysis of IP 6 (ε ∼ 7 ‰), where less than three P i were released. The incorporation of oxygen from water into P i during the hydrolysis of AMP and GPO 4 by phytase yielded a normal isotopic fractionation (ε ∼ −12 ‰), similar to values reported for acid phosphatases from potato and wheat germ. We attribute this similarity in ε to the same amino acid sequence motif (RHGXRXP) at the active site of these enzymes, which leads to similar reaction mechanisms. We suggest that the striking substrate dependency of the isotopic fractionation could be attributed to a difference in the δ 18 O values of the C-O-P bridging and non-bridging oxygen atoms in organic phosphate compounds.

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Phosphorus limitation in a Ferralsol: Impact on microbial activity and cell internal P pools

Cited by 108 publications

References 64 publications

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

Phosphorus distribution and availability in untreated and mechanically separated biogas digestates

The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and <i>Aspergillus niger</i> phytase

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Phosphorus limitation in a Ferralsol: Impact on microbial activity and cell internal P pools

Cited by 108 publications

References 64 publications

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

Phosphorus distribution and availability in untreated and mechanically separated biogas digestates

The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and &lt;i&gt;Aspergillus niger&lt;/i&gt; phytase

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The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and <i>Aspergillus niger</i> phytase