Phosphorus compounds in the cell. 1. Protein-bound phosphorus fractions studied with the aid of radioactive phosphorus

Toxic liver injury. Inhibition of protein synthesis in rat liver by dimethylnitrosamine in vivo

“…The concentrations and specific radioactivities of the plasma inorganic phosphorus, and liver acid-soluble, lipid and alkali-soluble phosphorus fractions (Davidson et al 1951) were determined. The concentrations and relative specific activities, calculated as shown, are given in Table 3.…”

“…This was determined by a modification of the method of Fiske & Subbarow (1925). Separation of the phosphorus-containing fractions of liver into acid-soluble, lipid and residual phosphorus was done by the procedure of Davidson, Frazer & Hutchison (1951), and partially purified ribonucleic acid (RNA) was prepared according to Davidson & Smellie (1952). Plasma inorganic phosphorus was precipitated by the method of Stoner et al (1952).…”

A Difference Between Lymph Nodal and Thymic Lymphocytes Shown by Transfusion of Labelled Cells

“…The Schmidt-Thannhuuser m ethod of isolating DNA has been criticized (Davidson, Frazer and Hutchinson [1951]). Assuming that isolation is incom plete, extraneous phosphorus-fractions m ay be included and spoil the results.…”

The recirculation of lymphocytes from blood to lymph in the rat

“…of the sodium citrate-formaldehyde solution and counted in a G-M liquid counter. Lipid was extracted from lymphocytes by the extraction procedure described by Davidson, Frazer & Hutchison (1951); the acid-soluble fraction was discarded and the final lipid extract dissolved in 10 ml. of chloroform and counted in a G-M liquid counter.…”