Phosphorus availability in a forest soil determined with a respiratory assay compared to chemical methods

Demetz, Matthias; Insam, Heribert

doi:10.1016/s0016-7061(98)00090-1

Cited by 49 publications

(21 citation statements)

References 20 publications

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“…This was in accordance to earlier report with Stevia rebaudiana Bertoni plant treated with TCP and PSB, where Burkholderia gladioli 10216 caused 332.02% increase of soluble P, and Enterobacter aerogenes resulted in 269.1% available P (Mamta et al 2010). The importance of phosphate solubilizing microorganisms is also considered in terms of P immobilized in the biomass of host plants (Demetz and Insam 1999). The stimulatory effect of the inoculation of PSB was evident on P uptake by A. hookeri Th.…”

Section: Treatmentssupporting

confidence: 92%

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Kshetri

Pandey

Sharma

2018

Journal of Plant Interactions

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This work describes integrated nutrient management for cultivation of Allium hookeri by using phosphate solubilizing bacteria (PSB) applied in rhizosphere, along with tricalcium phosphate (TCP). Arthrobacter luteolus S4C7, Enterobacter asburiae S5C7, Klebsiella pneumoniae S4C9, S4C10 and S6C1, and K. quasipneumoniae S6C2, were isolated from rhizosphere of Allium hookeri Thwaites, and were found to release substantial amount of soluble phosphate (124.8-266.4 μg/mL) from TCP in vitro conditions. These isolates were experimented for plant growth promoting attributes, including IAA, siderophore, and nitrogen-fixation. Treatment with PSB resulted in enhanced growth of A. hookeri Th., which was even better with TCP amendment with PSB. K.quasipneumoniae S6C2 resulted in 39.1% and 533.3% increase (p ≤ 0.05) of root length and weight respectively. The treatment with these isolates, in TCP amended soil also resulted in 200-250% increase in available P in soil, which was maximum for K. quasipneumoniae (1.866 mg/g). ARTICLE HISTORY

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Section: Treatmentssupporting

confidence: 92%

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Kshetri

Pandey

Sharma

2018

Journal of Plant Interactions

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“…4) may be related to organic P being less available. Demetz and Insam (1999) found that microbial growth rate was related to P availability and we expect that similar effects could be caused by variations in organic P quality. However, more detailed studies on both enzymatic activity of P degrading enzymes and organic P quality using for instance 31 P-NMR are needed in order to untangle the causes of the small but still lower labile P concentrations on small islands.…”

Section: Effects Of Fire History On P Forms and Availabilitymentioning

confidence: 78%

“…In order to estimate the nature of nutrient limitation of the microbial community, additions of N and P were made to the four incubation vessels prepared for each sample, using the approach described by Nordgren (1992); this approach has previously been used in several studies for estimating the level of availability of soil N and P to microbial organisms (e.g., Demetz and Insam 1999;Vesterdal 1998;Ilstedt et al 2003;Giesler et al 2004;Ilstedt and Singh 2005;Gnankambary et al 2007). Glucose ?…”

Section: Microbial Respirationmentioning

confidence: 99%

Soil phosphorus and microbial response to a long-term wildfire chronosequence in northern Sweden

et al. 2009

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In the prolonged absence of major disturbances, ecosystems may enter a stage of retrogression, which is characterized by decreased ecosystem process rates both above and belowground, and often reduced availability of phosphorus (P). Disturbance through wildfire can increase soil P losses through leaching or erosion, but in the long-term absence of fire, soil P could potentially become increasingly bound in more stable forms that are less available to microbes. We studied forms of P and microbial respiration kinetics in the humus layer of a group of islands that vary considerably in wildfire frequency (40-5,300 years since last fire), and which are known to enter retrogression in the prolonged absence of fire. We found a decrease in labile P with decreasing fire frequency but no change in total P. Soil microorganisms responded more strongly to N than to P addition, and microbial biomass N:P ratios remained unchanged across the gradient. However, the concentration of labile P was the best predictor of microbial respiration responses across the islands, and this provides some evidence that declining access to P could contribute to the decline in soil microbial activity during retrogression. Our results show that even though N is arguably the main limiting nutrient during retrogression in this chronosequence, long term absence of fire also causes a decline in P availability which negatively affects microbial activity. This in turn could potentially impair microbially driven processes such as decomposition and mineralization and further contribute to the reduced availability of soil nutrients during retrogression.

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“…High AMR after glucose addition is thus indicative of carbon limitation, while AMR which increases even further upon nutrient addition (e.g., nitrogen or phosphorus) is indicative of these nutrients being limiting factors for microbial growth (31). This technique (with minor modifications) has been used several times (9,11,12,23,25,30,31,34,35).…”

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confidence: 99%

Rapid Method of Determining Factors Limiting Bacterial Growth in Soil

Aldén

Demoling

Bååth

2001

Appl Environ Microbiol

204

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A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils: an agricultural soil and a forest humus soil. Carbon (as glucose) was found to be the limiting substance for bacterial growth in both of these soils. The effect of adding different amounts of nutrients was studied, and tests were performed to determine whether the additions affected the soil pH and subsequent bacterial activity. The incubation time required to detect bacterial growth after adding substrate to the soil was also evaluated. Second, the method was used in experiments in which three different size fractions of straw (1 to 2, 0.25 to 1, and <0.25 mm) were mixed into the agricultural soil in order to induce N limitation for bacterial growth. When the straw fraction was small enough (<0.25 mm), N became the limiting nutrient for bacterial growth after about 3 weeks. After the addition of the larger straw fractions (1 to 2 and 0.25 to 1 mm), the soil bacteria were C limited throughout the incubation period (10 weeks), although an increase in the thymidine incorporation rate after the addition of C and N together compared with adding them separately was seen in the sample containing the size fraction from 0.25 to 1 mm. Third, soils from high-pH, limestone-rich areas were examined. P limitation was observed in one of these soils, while tendencies toward P limitation were seen in some of the other soils.

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Phosphorus availability in a forest soil determined with a respiratory assay compared to chemical methods

Cited by 49 publications

References 20 publications

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Rhizosphere mediated nutrient management inAllium hookeriThwaites by using phosphate solubilizing rhizobacteria and tricalcium phosphate amended soil

Soil phosphorus and microbial response to a long-term wildfire chronosequence in northern Sweden

Rapid Method of Determining Factors Limiting Bacterial Growth in Soil

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