Phosphorus and nitrogen removal by a novel phosphate-accumulating organism, Arthrobacter sp. HHEP5 capable of heterotrophic nitrification-aerobic denitrification: Safety assessment, removal characterization, mechanism exploration and wastewater treatment

Zhang, Mengyu; Pan, Luqing; Liu, Liping; Su, Chen; Dou, Le; Su, Zhaopeng; He, Ziyan

doi:10.1016/j.biortech.2020.123633

Cited by 71 publications

(7 citation statements)

References 36 publications

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“…Over the past few decades, efforts have been devoted to isolating functional microorganisms in AS, such as nitrogen and phosphorus removing bacteria [11][12][13], strains leading to sludge flocculation [14], and harmful strains that cause sludge swelling and foaming [7,15]. Despite this, studies of microbial composition and microbial interactions at different stages of the long-term running WWTPs are still limited.…”

Section: Introductionmentioning

confidence: 99%

Community Composition and Function of Bacteria in Activated Sludge of Municipal Wastewater Treatment Plants

Xie

Zhong

Ouyang

et al. 2021

Water

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Municipal wastewater treatment plants (WWTPs) use functional microorganisms in activated sludge (AS) to reduce the environmental threat posed by wastewater. In this study, Illumina NovaSeq sequencing of 16S rRNA genes was performed to explore the microbial communities of AS at different stages of the two WWTP projects in Shenzhen, China. Results showed that Proteobacteria, Bacteroidetes, Acidobacteria, Firmicutes, and Nitrospirae were the dominant phyla in all the samples, with Proteobacteria being the most abundant and reaching a maximum proportion of 59.63%. There was no significant difference in biodiversity between the two water plants, but Stage 1 and Stage 2 were significantly different. The Mantel test indicated that nitrate, total nitrogen (TN), chemical oxygen demand (COD), and nutrients were essential factors affecting the bacterial community structure. FAPROTAX analysis emphasized that the leading functional gene families include nitrification, aerobic nitrite oxidation, human pathogens, and phototrophy. This study reveals changes in the community structure of AS in different treatment units of Banxuegang WWTP, which can help engineers to optimize the wastewater treatment process.

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Section: Introductionmentioning

confidence: 99%

Community Composition and Function of Bacteria in Activated Sludge of Municipal Wastewater Treatment Plants

Xie

Zhong

Ouyang

et al. 2021

Water

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“…Sniffen et al found that 9.18 mg/L of total nitrogen were removed per day in raw leachate by using the mixed culture of bacteria and algae 59 whereas Zhang et al observed that the phosphate-accumulating organism, Arthrobacter sp. HHEP 5 removed 99% of phosphorus and 99.37% of nitrate isolated from the mariculture effluents 60 . Wang et al demonstrated that the removal rate of total nitrogen and total phosphorus were 78.3% and 87.2% correspondingly after the treatment by Chlorella-exiguobacterium from the piggery wastewater treatment 61 .…”

Section: Discussionmentioning

confidence: 99%

In-situ remediation of nitrogen and phosphorus of beverage industry by potential strains Bacillus sp. (BK1) and Aspergillus sp. (BK2)

Bhambri¹,

Karn²,

Singh³

2021

Sci Rep

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The bioremediation of beverage (treated and untreated) effluent was investigated in the current study by using the potential strains of Bacillus sp. (BK1) and Aspergillus sp. (BK2). Effluent was collected from the beverage industry (initial concentration of nitrogen were 3200 ± 0.5 mg/L and 4400 ± 0.6 mg/L whereas phosphorus were 4400 ± 2 mg/L and 2600 ± 1 mg/L in treated and untreated effluent correspondingly). Further, the BK1 and BK2 exhibited high removal competence after 1 week of incubation; BK1 removed phosphorus 99.95 ± 0.7% and BK2 95.69 ± 1% in treated effluent while nitrogen removed about 99.90 ± 0.4% by BK1 and 81.25 ± 0.8% by BK2 (initial concentration of phosphorus 4400 ± 2 mg/L and nitrogen 3200 ± 0.5 mg/L). Next, in the untreated effluent BK1 removed 99.81 ± 1% and BK2 99.85 ± 0.8% of phosphorus while removed nitrogen 99.93 ± 0.5% by BK1 and 99.95 ± 1.2% by BK2 correspondingly, (initial concentration of phosphorus 2600 ± 1 mg/L and nitrogen 4400 ± 0.6 mg/L). The physiochemical composition of sample such as pH, total carbohydrates, total proteins, total solids of treated and untreated effluent were also analysed before and after treatment of both the samples. BK1 and BK2 increased the pH by 8.94 ± 0.3 and 9.5 ± 0.4 correspondingly in treated effluent whereas 6.34 ± 0.5 and 7.5 ± 0.2 correspondingly in untreated effluent (initial pH of treated and untreated effluent 7.07 ± 0.8 and 4.85 ± 0.3 correspondingly). Total Carbohydrates removed about 17,440 ± 4.6 mg/L and 10,680 ± 3.2 mg/L by BK1 and BK2 correspondingly in treated effluent whereas 18,050 ± 3.5 mg/L and 18,340 ± 2.3 mg/L correspondingly in untreated effluent (initial concentration of treated and untreated effluent 25,780 ± 1.6 mg/L and 35,000 ± 1.5 mg/L correspondingly) while BK1 and BK2 removed total proteins by 30.336 ± 4.6 mg/L and 40.417 ± 2.3 mg/L correspondingly in treated effluent whereas 18.929 ± 1.2 mg/L and 17.526 ± 0.8 mg/L correspondingly in untreated effluent (initial concentration of treated and untreated effluent 49.225 ± 1.5 mg/L and 20.565 ± 1 mg/L correspondingly). Next, total solids removed by BK1 and BK2 2.5 ± 0.3 mg/L and 1.6 ± 0.6 mg/L correspondingly in treated effluent whereas 5.5 ± 0.8 mg/L and 4.6 ± 0.6 mg/L in untreated effluent (initial concentration of treated and untreated effluent 5.6 ± 1.5 mg/L and 9.48 ± 1.2 mg/L correspondingly). Both the strains BK1 and BK2 are highly efficient in the nitrogen and phosphorus removal therefore this strain may be applied for the potential remediation.

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“…Figure 6a confirms the presence of target gene fragments in strain L2. The nitrification gene amoA (encoding ammonia nitrogen oxygenase, NH 4 + -N to NH 2 OH) contributes to the conversion of ammonia nitrogen to a high-oxidation form [58]. The hao gene encodes hydroxylamine dehydrogenase that oxidizes NH 2 OH to NO 2 − .…”

Section: Detection Of Genes Related To Nitrogen Removal In Hn-ad Stra...mentioning

confidence: 99%

Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

Li,

He,

Wang

et al. 2024

Water

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The biological denitrification of low-C/N wastewater is a great challenge in treatment plants due to the lack of microorganisms with heterotrophic nitrification–aerobic denitrification (HN-AD) abilities. In this study, Bacillus sp. L2 was isolated from aeration tank water samples using a nitrification medium and screened for its ability to perform HN-AD in low-C/N wastewater. The strain showed a maximum NH4+-N removal rate of 98.37% under low-C/N conditions. In the presence of a mixed N source, strain L2 was capable of completely removing NH4+-N within 24 h. Furthermore, optimal nitrogen removal conditions for strain L2 were found to be C/N = 9, pH = 9, and sodium acetate as the C source. Under optimal conditions, the strain was able to maintain a high NH4+-N removal rate under 0–3% salinity and an NH4+-N concentration of 200 mg/L or less. The denitrification pathways of strain L2 were NH4+→NH2OH→NO2−(↔NO3−)→NO→N2O→N2 and NH4+→NH2OH→NO→N2O→N2. Furthermore, semi-continuous wastewater treatment was conducted using immobilized technology, which resulted in more than 82% NH4+-N removal after three cycles of reuse. This study demonstrates the great potential of Bacillus sp. L2 in wastewater treatment applications.

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Phosphorus and nitrogen removal by a novel phosphate-accumulating organism, Arthrobacter sp. HHEP5 capable of heterotrophic nitrification-aerobic denitrification: Safety assessment, removal characterization, mechanism exploration and wastewater treatment

Cited by 71 publications

References 36 publications

Community Composition and Function of Bacteria in Activated Sludge of Municipal Wastewater Treatment Plants

Community Composition and Function of Bacteria in Activated Sludge of Municipal Wastewater Treatment Plants

In-situ remediation of nitrogen and phosphorus of beverage industry by potential strains Bacillus sp. (BK1) and Aspergillus sp. (BK2)

Heterotrophic Nitrification–Aerobic Denitrification by Bacillus sp. L2: Mechanism of Denitrification and Strain Immobilization

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