Phosphorolytic Reactivity of <i>o</i>-Iodosylcarboxylates and Related Nucleophiles

Morales‐Rojas, Hugo; Moss, Robert A.

doi:10.1021/cr9405462

Cited by 194 publications

(106 citation statements)

References 190 publications

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“…2-iodosyl-and 2-iodylbenzoates, may successfully be used for the degradation of organophosphorus substrates, including those mentioned above. 3 This is why we believe that our novel easy and fairly effective syntheses of 2-iodosyl-or 2-iodylbenzoic acids may be of a practical interest.…”

Section: Introductionmentioning

confidence: 99%

Optimized syntheses of iodylarenes from iodoarenes, with sodium periodate as the oxidant. Part II

Kraszkiewicz¹,

Skulski²

2003

Arkivoc

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An improved method is reported for optimized preparations of iodylarenes, ArIO 2 , from iodoarenes, ArI, using NaIO 4 as the oxidant dissolved in boiling 30% (v:v) aq. CH 3 COOH solutions. The former reaction times, reported in Part I, 1 were shortened from 8-16 hours to 3-6 hours, with preserving the same good yields and high purities of the crude final products, ArIO 2 . A considerably improved method of preparing "2-iodosylbenzoic acid" is also reported in this paper. See also our Warning submitted in Experimental.

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Section: Introductionmentioning

confidence: 99%

Optimized syntheses of iodylarenes from iodoarenes, with sodium periodate as the oxidant. Part II

Kraszkiewicz¹,

Skulski²

2003

Arkivoc

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“…The target of our directed evolution is the phosphotriesterase (PTE) from Pseudomonas Diminuta, an enzyme that detoxifies hazardous pesticides and nerve gas agents by hydrolysis 20,21 . By successful application of GSBs as vehicles for directed enzyme evolution of PTE, we establish the principles behind this approach and demonstrate its utility.…”

Section: Resultsmentioning

confidence: 99%

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

et al. 2014

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Natural evolution relies on the improvement of biological entities by rounds of diversification and selection. In the laboratory, directed evolution has emerged as a powerful tool for the development of new and improved biomolecules, but it is limited by the enormous workload and cost of screening sufficiently large combinatorial libraries. Here we describe the production of gel-shell beads (GSBs) with the help of a microfluidic device. These hydrogel beads are surrounded with a polyelectrolyte shell that encloses an enzyme, its encoding DNA and the fluorescent reaction product. Active clones in these manmade compartments can be identified readily by fluorescence-activated sorting at rates >107 GSBs per hour. We use this system to perform the directed evolution of a phosphotriesterase (a bioremediation catalyst) caged in GSBs and isolate a 20-fold faster mutant in less than one hour. We thus establish a practically undemanding method for ultrahigh-throughput screening that results in functional hybrid composites endowed with evolvable protein components. Evolution of Catalysts Caged in Biomimetic Gel-Shell Beads AbstractNatural evolution relies on improvement of biological entities by rounds of

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“…To enhance hydrolysis, various reagents such as nucleophiles, α-effect nucleophiles, metal complexes, metallomicelles, and biological catalysts and media such as micelles, microemulsions, solid supports, and latexes have been employed. [15][16][17][18] In the present study, we focused on the neutralization of methyl parathion (MP) by hydrolysis. The choice of this compound is mainly related to its extensive use in Algeria as a pesticide, often in contact with aquatic environments.…”

Section: Co-genotoxic Potential In Human Hepatoma Hepg2 Cells May Be mentioning

confidence: 99%

Hydrolytic decontamination of methyl parathion in the presence of 2-aminoethanol: Kinetics study

Doumandji¹,

Moussiden

Ihdene³

et al. 2018

Journal of Pesticide Science

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Methyl parathion (MP) decontamination by hydrolysis in aqueous solutions containing 2-aminoethanol (EA) was investigated using the UV-Visible and GC/MS techniques. The kinetics of the hydrolysis reaction was studied at pH values of 4, 7, and 9 in water at 75, 85, and 95°C, respectively. At various EA concentrations 60, 100, and 200 mg/L MP degradation followed pseudofirst order kinetics and was found to be strongly pH and temperature dependent. The rate of MP degradation accelerated significantly as pH increased. The conversion reached 93.5% after 90 min of hydrolysis at a pH of 9, as compared to 69.9% and 49.8% at pH values of 7 and 4, respectively. The MP degradation byproducts removed from aqueous solutions by solid phase microextraction (SPME) were identified. The main intermediate products were p-nitrophenol and O,O-dimethyl phosphorothioate. This study concludes that hydrolysis in the presence of EA is an effective process for decontaminating solutions containing MP.

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Phosphorolytic Reactivity of o-Iodosylcarboxylates and Related Nucleophiles

Cited by 194 publications

References 190 publications

Optimized syntheses of iodylarenes from iodoarenes, with sodium periodate as the oxidant. Part II

Optimized syntheses of iodylarenes from iodoarenes, with sodium periodate as the oxidant. Part II

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

Hydrolytic decontamination of methyl parathion in the presence of 2-aminoethanol: Kinetics study

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