Phosphoribulokinase mediates nitrogenase-induced carbon dioxide fixation gene repression in Rhodobacter sphaeroides

Farmer, Ryan M.; Tabita, F. Robert

doi:10.1099/mic.0.000160

Cited by 3 publications

(3 citation statements)

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“…BLAST search with PRK of cyanobacterium Synechococcus PCC7942 against archaeal protein database identified PRK homologues in Archaea. Photosynthetic PRKs are classified into two groups from photosynthetic bacteria and plant-type oxygenic phototrophs such as plants, algae and cyanobacteria41011. Archaeal PRK homologues show approximately 30% amino acid sequence identity with PRKs in photosynthetic organisms and form a clade clearly distinct from those of bacterial and plant-type PRKs in a phylogenetic tree (Fig.…”

Section: Resultsmentioning

confidence: 99%

A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea

et al. 2017

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Two enzymes are considered to be unique to the photosynthetic Calvin–Benson cycle: ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for CO2 fixation, and phosphoribulokinase (PRK). Some archaea possess bona fide RuBisCOs, despite not being photosynthetic organisms, but are thought to lack PRK. Here we demonstrate the existence in methanogenic archaea of a carbon metabolic pathway involving RuBisCO and PRK, which we term ‘reductive hexulose-phosphate' (RHP) pathway. These archaea possess both RuBisCO and a catalytically active PRK whose crystal structure resembles that of photosynthetic bacterial PRK. Capillary electrophoresis-mass spectrometric analysis of metabolites reveals that the RHP pathway, which differs from the Calvin–Benson cycle only in a few steps, is active in vivo. Our work highlights evolutionary and functional links between RuBisCO-mediated carbon metabolic pathways in methanogenic archaea and photosynthetic organisms. Whether the RHP pathway allows for autotrophy (that is, growth exclusively with CO2 as carbon source) remains unknown.

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Section: Resultsmentioning

confidence: 99%

A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea

et al. 2017

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“…Phosphoribulokinase (n. 59, spot 23), an enzyme that catalyzes the ATP‐dependent phosphorylation of ribulose‐5‐phosphate to ribulose‐1,5‐phosphate, may play a role in the carbon and nitrogen balance, as suggested by recent results obtained using Rhodobacter sphaeroides (Farmer & Tabita, ). Spot 23 also displayed a significant result for data linked to protein hydrolysate 2 (HP2).…”

Section: Discussionmentioning

confidence: 96%

Effects of different nitrogen fertilizers on two wheat cultivars: An integrated approach

et al. 2018

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Investigation of cultivated plant physiology grown under low energy input plays an important role to indicate their fitness to the new environmental conditions. The durum‐wheat cultivars Creso and Dylan were tested to evaluate the growth, production, and proteomic and transcriptomic profiles of the crop under different synthetic and organic nitrogen fertilization regimes. In this work, a two‐dimensional gel electrophoresis (2‐DE) approach combined with liquid chromatography–mass spectrometry (LC–MS) was used to investigate the protein changes induced by the use of different nitrogen sources (hydrolysate of proteins 1 and 2, rhizovit, synthesis, leather) on wheat plants. Proteomic studies were integrated with qPCR analysis of genes related to glutamine synthetase/glutamine‐2‐oxoglutarate aminotransferase (GS‐GOGAT) and tricarboxylic acid (TCA) metabolic pathways because most relevant for nitrogen‐dependent plants growth. The proteomic analysis lead to the isolation of 23 spots that were able to distinguish the analyzed samples. These spots yielded the identification of 60 proteins involved in photosynthesis, glycolysis, and nitrogen metabolism. As an example, the quinone oxidoreductase‐like protein and probable glutathione S‐transferase GSTU proteins were identified in two spots that represents the most statistically significant ones in Dylan samples. Transcript analysis indicated that related genes exhibited different expression trends; the heat map also revealed the different behaviors of the hydrolysates of the proteins 1 and 2 nitrogen sources. The effects of nitrogenous fertilizers at the proteomic and agronomic levels revealed that plants fertilized with synthesis or rhizovit gave the best results concerning yield, whereas rhizovit and protein hydrolysates were most effective for proteins content in the grain (% of dry weight). Therefore, all parameters measured in this study indicated that different kinds of nitrogen fertilization used have a relevant impact on plant growth and production.

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“…10 In nature, six major routes of CO 2 metabolic conversion (including the Calvin cycle, the reductive citric acid cycle, the dicarboxylate/4-hydroxybutyrate cycle, the 3-hydroxypropionate cycle, the 3-hydroxypropionate/4-hydroxybutyrate cycle, and the reductive acetylCoA route) have been reported, which are able to produce organic materials. 9,10 Directly adopting a single enzyme to catalyze CO 2 conversion by formate dehydrogenase (FDH), 11,12 carbon monodioxide dehydrogenase (CODH) 13,14 or nitrogenase 15,16 has also been reported. Furthermore, integration of several enzymes to implement multienzyme reactions by the oxidoreductases has been studied.…”

Section: Introductionmentioning

confidence: 99%