Phosphoproteomic analysis of duck egg yolk provides novel insights into its characteristics and biofunctions

Arshad, Rida; Meng, Yaqi; Qiu, Ning; Geng, Fang; Mine, Yoshinori; Keast, Russell; Zhu, Chunxia

doi:10.1002/jsfa.11453

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…It has been reported that dehydroepiandrosterone affected both protein synthesis and phosphorylation level to decrease fat deposition in broiler chickens during embryonic development [13]. Several phosphoproteomics studies have been performed on duck eggs, the phosphoproteins in duck egg white were mainly involved in the binding, transport activity, biological regulation, and metabolic processes [14], and the phosphoproteins in duck egg yolk were mainly involved in binding, catalytic, immune response, and metabolic activity [15]. In our study, phosphoproteomics was employed to dissect the molecular regulation mechanisms of F1 cross chicken cecum infected with S. Enteritidis.…”

Section: Introductionmentioning

confidence: 99%

Phosphoproteomics Profile of Chicken Cecum in the Response to Salmonella enterica Serovar Enteritidis Inoculation

Miao

Zhao

et al. 2022

Animals

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Salmonella enterica serovar Enteritidis (S. Enteritidis) is a foodborne pathogen, which can cause great threats to human health through the consumption of contaminated poultry products. This research combines TMT labeling, HPLC and mass-spectrometry-based phosphoproteomics on cecum of the F1 cross of Guangxi Yao chicken and Jining Bairi chicken. The treated group was inoculated with 0.3 mL inoculum S. Enteritidis, and the control group was inoculated with 0.3 mL phosphate-buffered saline (PBS). A total of 338 differentially phosphorylated modification sites in 243 differentially phosphorylated proteins (DPPs) were chosen for downstream analyses. A total of 213 sites in 146 DPPs were up-regulated and 125 sites in 97 DPPs were down-regulated. Functional analysis was performed for DPPs based on gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and the protein domain. The DPPs were mainly enriched in immune- and metabolic-related GO-BP (biological process) and KEGG pathways. We predicted and classified the subcellular structure and COG/KOG of DPPs. Furthermore, protein–protein interaction network analyses were performed by using multiple algorithms. We identified 71 motifs of the phosphorylated modification sites and selected 18 sites randomly to detect the expression level through parallel reaction monitoring (PRM). S. Enteritidis inoculation caused phosphorylation alteration in immune- and metabolic-related proteins. The invasion of S. Enteritidis may be actualized by inducing cecum cell apoptosis through the endoplasmic reticulum pathway, and chickens could resist the invasion of S. Enteritidis by affecting the function of ECM receptors. The findings herein provide a crucial theoretical foundation to understand the molecular mechanism and epigenetic regulation in response to S. Enteritidis inoculation in chickens.

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Section: Introductionmentioning

confidence: 99%