Phosphoproteome Dynamics Upon Changes in Plant Water Status Reveal Early Events Associated With Rapid Growth Adjustment in Maize Leaves

Bonhomme, Ludovic; Valot, Benoı̂t; Tardieu, François; Zivy, Michel

doi:10.1074/mcp.m111.015867

Cited by 95 publications

(108 citation statements)

References 59 publications

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“…They are compatible with hydraulic processes but also with posttranslational protein modifications such as phosphorylation/dephosphorylation (Novak et al, 2010;Bonhomme et al, 2012) or with the transfer of a plant hormone such as ABA over short distances from the apoplast to the symplast (Hartung et al, 2002). They are not necessarily compatible with mechanisms involving the synthesis of new transcripts and protein, the trafficking of the latter toward sites of action, and their effects on growth.…”

Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 75%

“…In an experiment involving a similar protocol of soil rehydration to that in Figure 4, with the same maize line, Bonhomme et al (2012) showed that 40% of the phosphopeptides affected by water deficit displayed half-recovery of their phosphorylation status during the first 1 h of rehydration. Three and six phosphorylation sites recovered as early as 5 and 10 min after rewatering, respectively.…”

Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 99%

“…It might also involve rapid changes in cell wall properties triggered by a hydraulic signal. Indeed, the rapid phosphorylation events observed by Bonhomme et al (2012) may trigger rapid changes in cell wall mechanical properties. They also could affect the activity of PIP proteins (Maurel et al, 2008;Van Wilder et al, 2008).…”

Section: The Hydraulic Trigger Of Changes In Ler Does Not Rule Out Nomentioning

confidence: 99%

“…The proposed mechanism of elongation control during rapid changes of environmental conditions was then tested with a model. Finally, other putative controls were analyzed via a temporal series of genome-wide transcriptomes, presented in this study, and of a phosphoproteome published in a companion paper (Bonhomme et al, 2012). Table S1) were classified into a limited number of patterns in each experiment, according to the evaporative demand and water availability measured on the considered day.…”

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confidence: 99%

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A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

et al. 2014

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Plants are constantly facing rapid changes in evaporative demand and soil water content, which affect their water status and growth. In apparent contradiction to a hydraulic hypothesis, leaf elongation rate (LER) declined in the morning and recovered upon soil rehydration considerably quicker than transpiration rate and leaf water potential (typical half-times of 30 min versus 1–2 h). The morning decline of LER began at very low light and transpiration and closely followed the stomatal opening of leaves receiving direct light, which represent a small fraction of leaf area. A simulation model in maize (Zea mays) suggests that these findings are still compatible with a hydraulic hypothesis. The small water flux linked to stomatal aperture would be sufficient to decrease water potentials of the xylem and growing tissues, thereby causing a rapid decline of simulated LER, while the simulated water potential of mature tissues declines more slowly due to a high hydraulic capacitance. The model also captured growth patterns in the evening or upon soil rehydration. Changes in plant hydraulic conductance partly counteracted those of transpiration. Root hydraulic conductivity increased continuously in the morning, consistent with the transcript abundance of Zea maize Plasma Membrane Intrinsic Protein aquaporins. Transgenic lines underproducing abscisic acid, with lower hydraulic conductivity and higher stomatal conductance, had a LER declining more rapidly than wild-type plants. Whole-genome transcriptome and phosphoproteome analyses suggested that the hydraulic processes proposed here might be associated with other rapidly occurring mechanisms. Overall, the mechanisms and model presented here may be an essential component of drought tolerance in naturally fluctuating evaporative demand and soil moisture.

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Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 75%

Section: A Transcriptome Analysis Suggests Possible Contributions Of mentioning

confidence: 99%

Section: The Hydraulic Trigger Of Changes In Ler Does Not Rule Out Nomentioning

confidence: 99%

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confidence: 99%

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A Hydraulic Model Is Compatible with Rapid Changes in Leaf Elongation under Fluctuating Evaporative Demand and Soil Water Status

et al. 2014

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“…However, these are specific for osmotic stress and are not induced by salt stress, although this stress also has an osmotic component. While some studies attempted to study molecular responses at different stress levels, this was usually done in progressive soil-drying experiments (Cramer et al, 2007;Harb et al, 2010;Bonhomme et al, 2012), where the time factor cannot be separated from the stress severity factor. To our knowledge, no dedicated controlled studies have been performed on the effects of stress severity on gene expression.…”

Section: Current Stress Marker Genes Are Indicators Of Severe Stress mentioning

confidence: 99%

What Is Stress? Dose-Response Effects in Commonly Used in Vitro Stress Assays

et al. 2014

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In vitro stress assays are commonly used to study the responses of plants to abiotic stress and to assess stress tolerance. A literature review reveals that most studies use very high stress levels and measure criteria such as germination, plant survival, or the development of visual symptoms such as bleaching. However, we show that these parameters are indicators of very severe stress, and such studies thus only provide incomplete information about stress sensitivity in Arabidopsis (Arabidopsis thaliana). Similarly, transcript analysis revealed that typical stress markers are only induced at high stress levels in young seedlings. Therefore, tools are needed to study the effects of mild stress. We found that the commonly used stress-inducing agents mannitol, sorbitol, NaCl, and hydrogen peroxide impact shoot growth in a highly specific and dose-dependent way. Therefore, shoot growth is a sensitive, relevant, and easily measured phenotype to assess stress tolerance over a wide range of stress levels. Finally, our data suggest that care should be taken when using mannitol as an osmoticum.

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