Phosphoprotein Phosphatase Function of Secreted Purple Acid Phosphatases

Ghahremani, Mina; Plaxton, William C.

doi:10.1007/978-3-030-48733-1_2

Cited by 3 publications

(1 citation statement)

References 50 publications

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“…Plant PAPs are typically composed of homodimers with subunits that weigh around 50–70 kDa, which is classified as high molecular weight (HMW) PAPs. In contrast, mammalian PAPs exist as monomers weighing approximately 35 kDa and are classified as low molecular weight (LMW) PAPs (Ghahremani & Plaxton, 2020; Schenk et al., 2013). However, it is noteworthy that LMW ‘mammalian‐like’ PAPs have been found in plants, and a gene encoding a HMW ‘plant‐like’ PAP has been identified in the human genome (Feder et al., 2020; Flanagan et al., 2006; O'Gallagher et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Multi‐omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

Luo,

Chen,

Huang

et al. 2023

The Plant Journal

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SUMMARYStylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the crucial APase responsible for this variation remains unidentified. In this study, transcriptomic and proteomic analyses were employed to identify eight Pi starvation‐inducible (PSI) APases belonging to the purple APase (PAP) family in the roots of stylo and seven in the leaves. Among these PSI‐PAPs, SgPAP7 exhibited a significantly positive correlation in its expression levels with the activities of both internal APase and root‐associated APase across 20 stylo genotypes under low‐Pi conditions. Furthermore, the recombinant SgPAP7 displayed high catalytic activity toward adenosine 5′‐diphosphate (ADP) and phosphoenolpyruvate (PEP) in vitro. Overexpression (OE) of SgPAP7 in Arabidopsis facilitated exogenous organic phosphorus utilization. Moreover, SgPAP7 OE lines showed lower shoot ADP and PEP levels than the wild type, implying that SgPAP7 is involved in the catabolism and recycling of endogenous ADP and PEP, which could be beneficial for plant growth in low‐Pi soils. In conclusion, SgPAP7 is a key gene with a major role in stylo adaptation to low‐Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources. It may also function as a PEP phosphatase involved in a glycolytic bypass pathway that minimizes the need for adenylates and Pi. Thus, SgPAP7 could be a promising target for improving tolerance of crops to low‐Pi availability.

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Section: Introductionmentioning

confidence: 99%

Multi‐omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

Luo,

Chen,

Huang

et al. 2023

The Plant Journal

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Recent insights into the metabolic adaptations of phosphorus-deprived plants

Dissanayaka

Ghahremani

Siebers

et al. 2020

Journal of Experimental Botany

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Orthophosphate (H2PO4 −, Pi) is an essential macronutrient required for many fundamental processes in plants, including photosynthesis and respiration, as well as nucleic acid, protein, and membrane phospholipid synthesis. The immense use of Pi-containing fertilizers in agriculture demonstrates how the soluble Pi levels of most soils are suboptimal for crop growth. The aim of this review is to explore recent and exciting advances concerning our understanding of adaptive metabolic processes that plants have evolved to alleviate the negative impact of nutritional Pi deficiency. Plant Pi starvation responses arise from complex signaling pathways that integrate altered gene expression with post-transcriptional and post-translational mechanisms. The resultant remodeling of the transcriptome, proteome, and metabolome enhances the efficiency of root Pi acquisition from the soil, as well as the use of assimilated Pi throughout the plant. We emphasize how the upregulation of high affinity Pi transporters and intra- and extracellular Pi scavenging and recycling enzymes, organic acid anion efflux, membrane remodeling, and the remarkable flexibility of plant metabolism and bioenergetics contribute to the survival of Pi-deficient plants. This research field is enabling development of a broad range of innovative and promising strategies for engineering P-efficient crops. Such cultivars are urgently needed to reduce inputs of unsustainable and nonrenewable Pi fertilizers for maximum agronomic benefit, and long-term global food security and ecosystem preservation.

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Arabidopsis PAP17 is a dual-localized purple acid phosphatase up-regulated during phosphate deprivation, senescence, and oxidative stress

O'Gallagher

Ghahremani

Stigter

et al. 2021

Journal of Experimental Botany

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A 35 kDa monomeric purple acid phosphatase (APase) was purified from cell wall extracts of Pi starved (–Pi) Arabidopsis thaliana suspension cells and identified as AtPAP17 (At3g17790) by mass spectrometry and N-terminal microsequencing. AtPAP17 was de novo synthesized and dual-localized to the secretome and/or intracellular fraction of –Pi or salt stressed plants, or senescing leaves. Transiently expressed AtPAP17-GFP localized to lytic vacuoles of the Arabidopsis suspension cells. No significant biochemical or phenotypical changes associated with AtPAP17 loss-of-function were observed in an atpap17 mutant during Pi deprivation, leaf senescence, or salinity stress. Nevertheless, AtPAP17 is hypothesized to contribute to Pi metabolism owing to its marked upregulation during Pi starvation and leaf senescence, broad APase substrate selectivity and pH-activity profile, and rapid repression and turnover following Pi-resupply to –Pi plants. While AtPAP17 also catalyzed the peroxidation of luminol, which was optimal at pH 9.2, it exhibited a low Vmax and affinity for hydrogen peroxide relative to horseradish peroxidase. These results, coupled with absence of a phenotype in the salt stressed or –Pi atpap17 mutant, do not support proposals that AtPAP17’s peroxidase activity contributes to the detoxification of reactive oxygen species during stresses that trigger AtPAP17 upregulation.

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Phosphoprotein Phosphatase Function of Secreted Purple Acid Phosphatases

Cited by 3 publications

References 50 publications

Multi‐omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

Multi‐omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

Recent insights into the metabolic adaptations of phosphorus-deprived plants

Arabidopsis PAP17 is a dual-localized purple acid phosphatase up-regulated during phosphate deprivation, senescence, and oxidative stress

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