Phosphomevalonate kinase regulates the MVA/MEP pathway in mango during ripening

Pathak, Garima; Dudhagi, Shivanand Suresh; Raizada, Saumya; Singh, Rajesh Kumar; Sane, Aniruddha P.; Sane, Vidhu A.

doi:10.1016/j.plaphy.2023.01.030

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…For the other genes involved in the MVA pathway, almost all decreased gradually during the development of leaves in B. balsamifera, including one BbHMGCs, seven BbHMGCRs, and one BbMVK. However, the expression levels of two BbPMKs increased gradually in the late development stages, consistent with MiPMK in mango during ripening [39]. One BbMVD also displayed a similar expression trend from BBLII to BBLV.…”

Section: Discussionsupporting

confidence: 70%

Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.

Ju,

Liang,

Zheng

et al. 2024

Genes

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Blumea balsamifera (L.) DC., an important economic and medicinal herb, has a long history of being used as a traditional Chinese medicine. Its leaves have always been used as a raw material for the extraction of essential oils, comprising large amounts of terpenoids, which have good therapeutic effects on many diseases, such as eczema, bacterial infection, and hypertension. However, the genetic basis of terpenoid biosynthesis in this plant is virtually unknown on account of the lack of genomic data. Here, a combination of next-generation sequencing (NGS) and full-length transcriptome sequencing was applied to identify genes involved in terpenoid biosynthesis at five developmental stages. Then, the main components of essential oils in B. balsamifera were identified using GC–MS. Overall, 16 monoterpenoids and 20 sesquiterpenoids were identified and 333,860 CCS reads were generated, yielding 65,045 non-redundant transcripts. Among these highly accurate transcripts, 59,958 (92.18%) transcripts were successfully annotated using NR, eggNOG, Swissprot, KEGG, KOG, COG, Pfam, and GO databases. Finally, a total of 56 differently expressed genes (DEGs) involved in terpenoid biosynthesis were identified, including 38 terpenoid backbone genes and 18 TPSs, which provide a significant amount of genetic information for B. balsamifera. These results build a basis for resource protection, molecular breeding, and the metabolic engineering of this plant.

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Section: Discussionsupporting

confidence: 70%

Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.

Ju,

Liang,

Zheng

et al. 2024

Genes

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“…VIGR technology exploits the ability of viruses to multiply in plants and the plant antiviral defence system to overexpress (virus-induced gene overexpression (VIGO)) and silence (VIGS) target genes in plants (Paudel et al, 2022). Tobacco rattle virus (TRV) and apple latent spherical virus (ALSV) based VIGR systems are two of the most common systems used for VIGS and VIGO in fruit trees such as peach, apple, European pear, Japanese pear, almond and mango (Paudel et al, 2022;Pathak et al, 2023). In fruit trees, VIGR systems have been mostly used for functional genomics to characterise genes regulating different biological pathways such as carotenoid accumulation and fruit ripening in peach (Li et al, 2017) and mango (Pathak et al, 2023), pathogen resistance in peach (Cui and Wang, 2017), and flowering in apple, citrus, Japanese pear and Vitis spp.…”

Section: Functional Genetics For Trait Characterisation and Improvementmentioning

confidence: 99%

Advancing tree genomics to future proof next generation orchard production

Kerr,

Shehnaz,

Paudel

et al. 2024

Front. Plant Sci.

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The challenges facing tree orchard production in the coming years will be largely driven by changes in the climate affecting the sustainability of farming practices in specific geographical regions. Identifying key traits that enable tree crops to modify their growth to varying environmental conditions and taking advantage of new crop improvement opportunities and technologies will ensure the tree crop industry remains viable and profitable into the future. In this review article we 1) outline climate and sustainability challenges relevant to horticultural tree crop industries, 2) describe key tree crop traits targeted for improvement in agroecosystem productivity and resilience to environmental change, and 3) discuss existing and emerging genomic technologies that provide opportunities for industries to future proof the next generation of orchards.

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“…Key candidate genes associated with the biosynthesis of phenylpropanoids and flavonoids in C. cassia were identified from three tissue types: bark, leaves, and branches [16]. The mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways are two alternative, compartmentally separated pathways in terpenoid biosynthesis [17,18]. The MEP pathway mainly produces mono-and diterpenes, whereas the MVA pathway is associated with the formation of sesquiterpenes, triterpenes, and sterols [19].…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptomics and metabolomics analysis provides insights into aromatic volatiles formation in Cinnamomum cassia bark at different harvesting times

Yao,

Tan,

Huang

et al. 2024

BMC Plant Biol

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Background Cinnamomum cassia Presl, classified in the Lauraceae family, is widely used as a spice, but also in medicine, cosmetics, and food. Aroma is an important factor affecting the medicinal and flavoring properties of C. cassia, and is mainly determined by volatile organic compounds (VOCs); however, little is known about the composition of aromatic VOCs in C. cassia and their potential molecular regulatory mechanisms. Here, integrated transcriptomic and volatile metabolomic analyses were employed to provide insights into the formation regularity of aromatic VOCs in C. cassia bark at five different harvesting times. Results The bark thickness and volatile oil content were significantly increased along with the development of the bark. A total of 724 differentially accumulated volatiles (DAVs) were identified in the bark samples, most of which were terpenoids. Venn analysis of the top 100 VOCs in each period showed that twenty-eight aromatic VOCs were significantly accumulated in different harvesting times. The most abundant VOC, cinnamaldehyde, peaked at 120 months after planting (MAP) and dominated the aroma qualities. Five terpenoids, α-copaene, β-bourbonene, α-cubebene, α-funebrene, and δ-cadinene, that peaked at 240 MAP could also be important in creating C. cassia’s characteristic aroma. A list of 43,412 differentially expressed genes (DEGs) involved in the biosynthetic pathways of aromatic VOCs were identified, including phenylpropanoids, mevalonic acid (MVA) and methylerythritol phosphate (MEP). A gene-metabolite regulatory network for terpenoid and phenylpropanoid metabolism was constructed to show the key candidate structural genes and transcription factors involved in the biosynthesis of terpenoids and phenylpropanoids. Conclusions The results of our research revealed the composition and changes of aromatic VOCs in C. cassia bark at different harvesting stages, differentiated the characteristic aroma components of cinnamon, and illuminated the molecular mechanism of aroma formation. These foundational results will provide technical guidance for the quality breeding of C. cassia.

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Phosphomevalonate kinase regulates the MVA/MEP pathway in mango during ripening

Cited by 7 publications

References 34 publications

Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.

Full-Length Transcriptome Sequencing and RNA-Seq Analysis Offer Insights into Terpenoid Biosynthesis in Blumea balsamifera (L.) DC.

Advancing tree genomics to future proof next generation orchard production

Integrated transcriptomics and metabolomics analysis provides insights into aromatic volatiles formation in Cinnamomum cassia bark at different harvesting times

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