Phosphomannans and Other Components of Flocculent and Non-Flocculent Walls of Saccharomyces cerevisiae

Mill, P. J.

doi:10.1099/00221287-44-3-329

Cited by 77 publications

(28 citation statements)

References 19 publications

(14 reference statements)

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“…Glycosyl phosphate (mannosyl phosphate and glucosyl phosphate) substitutes have been described in yeast N-linked oligosaccharides (Mill, 1966;Hernandez et 1989) and also in lipophosphoglycans (LPG) from Leishmania major (McConville and Homans, 1992).…”

Section: Discussionmentioning

confidence: 99%

Glycosylinositol-phosphoceramide in the free-living protozoan Paramecium primaurelia: modification of core glycans by mannosyl phosphate.

Azzouz¹,

Striepen²,

Gerold³

et al. 1995

The EMBO Journal

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Glycolipids synthesized in a cell‐free system prepared from the free‐living protozoan Paramecium primaurelia and labelled with [3H]mannose and [3H]glucosamine using GDP‐[3H]mannose and UDP‐[3H]N‐acetyl glucosamine, respectively, were identified and structurally characterized as glycosylinositol‐phosphoceramides (GIP‐ceramides). The ceramide‐based lipid was also found in the GIP membrane anchor of the G surface antigen of P.primaurelia, strain 156. Using a combination of in vitro labelling with GDP‐[3H]mannose and in vivo labelling with 33P, we found that the core glycans of the P.primaurelia GIP‐ceramides were substituted with an acid‐labile modification identified as mannosyl phosphate. The modification of the glycosylinositol‐phospholipid core glycan by mannosyl phosphate has not been described to date in other organisms. The biosynthesis of GIP‐ceramide intermediates in P.primaurelia was studied by a pulse‐chase analysis. Their structural characterization is reported. We propose the following structure for the putative GIP‐ceramide membrane anchor precursor of P.primaurelia surface proteins: ethanolamine phosphate‐6Man‐alpha 1–2Man‐alpha 1–6Man‐(mannosyl phosphate)‐alpha 1–4glucosamine‐inositol‐phosphoceramide.

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Section: Discussionmentioning

confidence: 99%

Glycosylinositol-phosphoceramide in the free-living protozoan Paramecium primaurelia: modification of core glycans by mannosyl phosphate.

Azzouz¹,

Striepen²,

Gerold³

et al. 1995

The EMBO Journal

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“…Once developed, flocculation is a property intrinsic to cell-walls, isolated walls continuing to show flocculence (Masschelein and Devreux, 1957;Mill, 1966). The cell-wall structure of Succhuromyces cerevisiae, recently reviewed by Fleet (1 990), may be summarily described as a layer of insoluble P-glucan fibrils (Kopecka et al, 1974), overlaid by mannoproteins (mannan) and amorphous P-glucans (Kopecka et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Yeast flocculation: Flocculation onset and receptor availability

Stratford

1993

Yeast

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Flocculent strains of brewing yeast grow and ferment as single cells and flocculate in the stationary phase of growth. The switch from single-celled yeast growth to multi-celled aggregation, flocculation onset, is of critical importance to the brewing industry. Yeast flocculation involves adhesion of surface-lectins on flocculent cells to carbohydrate receptors on neighbouring cell-walls. The presence of carbohydrate receptors, outer-chain mannan side-branches, was monitored throughout growth of flocculent and non-flocculent strains of Saccharomyces cerevisiae, with particular attention to the growth phases where flocculation is normally developed. Receptors were probed by coflocculation with flocculent strains and by aggregation with concanavalin A, a lectin shown to use the same receptors as flocculation. While considerable variation was found in coflocculation and concanavalin A aggregation between strains, little or no change in receptor availability was found throughout the growth of all yeast strains. Yeast cells could easily be coflocculated at any growth stage. It was concluded that receptor availability is not involved in the process of flocculation onset.

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“…The chemical composition of the yeast cell wall changes during the development of the yeast (Bonaly et al, 1971) and flocculation phenomena depend directly on the components of the wall (Masschelein et al, 1963). The nature of these cell wall components and the effect that they have are the subject of contrasting theories concerning the phenomenon of yeast flocculation (Lyons & Hough, 1970, 1971Griffin & MacWilliam, 1969;Mill, 1966).…”

Section: Introductionmentioning

confidence: 99%

Yeast Flocculation: Influence of Nutritional Factors on Cell Wall Composition

Amri

Bonaly

Duteurtre³

et al. 1982

Microbiology

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The walls of two strains of Saccharomyces uvarum (flocculent and non-flocculent) were isolated from exponential and stationary phase cells after growth in media of different Ca2+ and K+ composition. Flocculation of the walls was identical to that of intact cells. The transition from the non-flocculent to the flocculent state involved alterations of the polysaccharide, protein and mineral (Ca2+ and K+) composition of the walls. The walls of flocculent cells had a higher mannose/glucose molar ratio, a lower percentage of protein, a lower percentage of Ca2+ and a higher percentage of K+.

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Phosphomannans and Other Components of Flocculent and Non-Flocculent Walls of Saccharomyces cerevisiae

Cited by 77 publications

References 19 publications

Glycosylinositol-phosphoceramide in the free-living protozoan Paramecium primaurelia: modification of core glycans by mannosyl phosphate.

Glycosylinositol-phosphoceramide in the free-living protozoan Paramecium primaurelia: modification of core glycans by mannosyl phosphate.

Yeast flocculation: Flocculation onset and receptor availability

Yeast Flocculation: Influence of Nutritional Factors on Cell Wall Composition

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