Phospholipids: synthesis, sorting, subcellular traffic - the yeast approach

Kohlwein, Sepp D.; Daum, Günther; Schneiter, Roger; Paltauf, F.

doi:10.1016/0962-8924(96)10025-8

Cited by 28 publications

(14 citation statements)

References 50 publications

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“…They have been found to be evenly distributed in the apical and basolateral domains in epithelial cells [82]. Trafficking of free GPIs to various cellular locations could occur via any of the traffic options available to lipids [83,84] including lipid transfer carrier proteins, contact-site-mediated transfer, and vesicular transport [15].…”

Section: Intracellular Distribution Of Gpi-apsmentioning

confidence: 99%

The GPI-anchor and protein sorting

Chatterjee¹,

Mayor²

2001

CMLS, Cell. Mol. Life Sci.

170

126

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Glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) are a diverse class of proteins that are anchored to the membrane solely via means of a posttranslational lipid modification, the GPI-moiety. Since their discovery in the late 1970s, years of research have provided significant insight into the functions of this ubiquitous modification. In addition to the structure and biosynthesis of the GPI-moiety, perhaps the best-studied feature of this glycolipid is its ability to impart characteristic membrane-trafficking properties to the proteins that it anchors. Study of the mechanism of sorting of GPI-APs has brought to light the importance of lateral heterogeneities in cell membranes, termed rafts, in biological sorting processes. The focus of this review is to examine the emerging role of the GPI-anchor and mechanisms involved in GPI-AP sorting in the context of intracellular trafficking pathways.

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Section: Intracellular Distribution Of Gpi-apsmentioning

confidence: 99%

The GPI-anchor and protein sorting

Chatterjee¹,

Mayor²

2001

CMLS, Cell. Mol. Life Sci.

170

126

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“…Phospholipid components of cellular membranes control membrane permeability and hence its efficiency as a barrier. Phospholipids contain phosphate molecules, their biosynthesis and transport require ATE CTP or CDP (Kohlwein et al 1996, Moreau et al 1998). Thus both low Pi and ATP concentration may affect phospholipid content of plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

The effect of phosphate deficiency on membrane phospholipid composition of bean (Phaseolus vulgaris L.) roots

1999

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Plasma membranes were isolated from ro~ts of bean (Phaseolus vulgaris L.) plants cultured on phosphate sufficient or phosphate deficient medium. The phospholipid composition of plasma membranes was analyzed and compared with that of the microsomal fraction. Phosphate deficiency had no influence on lipid/protein ratio in microsomal as well as plasma membrane fraction. In phosphate deficient roots phospholipid content was lower in the plasma membrane, but did not change in the microsomal fraction. Phosphatidylcholine and phosphatidylethanolamine were two major phospholipids in plasmalemma and microsomal membranes (80 % of the total). After two weeks of phosphate starvation a considerable decrease (about 50 %) in phosphatidylcholine and phosphatidylethanolamine in microsomat membranes was observed. The decline in two major phospholipids was accompanied by an increase in phosphatidic acid and lysophosphatidylcholine content. The effect of alterations in plasma membrane phospholipids on membrane fnnction e.g. nitrate uptake is discussed.

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“…Furthermore, lipids can be distributed asymmetrically across the leaflets of a membrane bilayer (67). Thus, the concerted synthesis, degradation, modification, and trafficking of lipids generate and sustain the highly heterogeneous lipid composition of organellar membranes (68)(69)(70). Mitochondria are organelles of endosymbiotic origin that, over evolution, have transferred most of their protein encoding genes into the cell host nucleus, with only a few genes still residing in the mitochondrial DNA (mtDNA) (71).…”

Section: Making Mitochondrial Membranes: the Contribution Of Er-mitocmentioning

confidence: 99%

Lipid synthesis and membrane contact sites: a crossroads for cellular physiology

Fernández-Murray

McMaster

2016

Journal of Lipid Research

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high throughput analyses, makes this yeast a powerful organism to unveil the intricacies of this field. The yeast S. cerevisiae has played a foundational role in the field of molecular and cellular biology of lipids, including the identification of membrane contact sites (MCSs) and how they link lipid metabolism with organelle dynamics. We present advances in two topics where extensive and outstanding contributions have recently been made where their relevance transcends the yeast lipid field. We first review new information about the phosphatidic acid (PA) phosphatase, Pah1, the yeast homolog of human lipin, and its role in directing lipid flux into membrane synthesis or storage. We review the contribution of Pah1 to lipid droplet (LD) formation and its interaction with the seipin complex, Fld1/Ldb16, to regulate endoplasmic reticulum (ER)-LD contact site dynamics. Second, we recapitulate recent developments revealing MCS connections between the ER and mitochondria with other cellular compartments, and how these intersect with the maintenance of lipid homeostasis. Furthermore, we explore MCS redundancies that allow cells to cope with changing metabolic demand. PA METABOLISM CO-COORDINATES MEMBRANE AND LD SYNTHESISPA is the common precursor for the synthesis of membrane phospholipids (PLs) and the major component of The pervasive importance of lipids in cell biology has become evident. From structural roles defining cellular compartments and surfaces with specific biological properties to functional roles in signal transduction processes and modulation of regulatory networks, the involvement of lipids in varied cellular functions is well-established. Concurrently, we have gained a broad understanding about the repertoire of proteins involved in synthesis, degradation, transport, and sensing of lipids, as well as the regulatory mechanisms that interconnect lipid metabolism with other cellular processes. The amenability of Saccharomyces cerevisiae to classical approaches of molecular genetics, and toThe work on the science described in this review was supported by a Discovery Grant from the Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada to C.R.M. Manuscript received 19 July 2016 and in revised form 6 August 2016. Published, JLR Papers in Press, August 12, 2016 DOI 10.1194 Lipid synthesis and membrane contact sites: a crossroads for cellular physiology Abbreviations: cER, cortical endoplasmic reticulum; ChiMERA, construct helping in mitochondria-endoplasmic reticulum association; CL, cardiolipin; DAG, diacylglycerol; EMC, endoplasmic reticulum membrane protein complex; ER, endoplasmic reticulum; ERMES, endoplasmic reticulum-mitochondria encounter structure; HOPS, homotypic fusion and vacuole protein sorting; Lam, lipid transfer protein anchored at a membrane contact site; LD, lipid droplet; Ltc, lipid transfer at contact site; LTP, lipid transfer protein; Mcp, maintenance of mitochondrial morphology 10 complementing protein; M...

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Phospholipids: synthesis, sorting, subcellular traffic - the yeast approach

Cited by 28 publications

References 50 publications

The GPI-anchor and protein sorting

The GPI-anchor and protein sorting

The effect of phosphate deficiency on membrane phospholipid composition of bean (Phaseolus vulgaris L.) roots

Lipid synthesis and membrane contact sites: a crossroads for cellular physiology

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