Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Hyperplastic nodules and hepatomas were induced in livers of rats fed a diet containing 0.05% N-2-fluorenylacetamide (2-FAA). The lipid contents, and phospholipid and fatty acid compositions were analyzed in plasma membranes (PM's) isolated from these tissues and normal rat liver, and the following trends were observed. The molar ratio of cholesterol to phospholipid-phosphorus (phospholipid-P) increased in the order: hepatoma less than normal liver less than hyperplastic nodules. The molar percentage of plasmalogen to phospholipid-P decreased in the order: hepatoma = hyperplastic nodules greater than normal liver. The percentages of choline phosphoglycerides (sum of phosphatidylcholine and lysophosphatidylcholine) and ethanolamine phosphoglycerides (sum of phosphatidylethanolamine and lysophosphatidylethanolamine) both decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. On the other hand, the percentages of sphingomyelin and phosphatidylserine both increased in the order: hepatoma less than hyperplastic nodules less than normal liver. As regards fatty acid composition, the percentages of both 18:1 and 18:2 decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. Those of 18:0 and 20:4 increased in the order: hepatoma less than hyperplastic nodules less than normal liver. These results suggested that the lipid bilayer in PM of hyperplastic nodules has characteristics roughly intermediate between those of hepatoma and liver PM's, although the molar ratio of cholesterol to phospholipid-P in hyperplastic nodules PM was not intermediate.

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Changes in the cell membrane of erythrocytes in tumor-bearing rats.

Fujii

Koizumi

Tamiya‐Koizumi

et al. 1984

Cell Struct. Funct.

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ABSTRACT. Cell electrophoresis of erythrocytes taken from AH 7974 hepatoma-bearing rats showed a marked decrease in the surface negative charge, as represented by a decrease in cell mobility. This change in the erythrocyte membrane began during the stationary phase of growth of transplanted tumor cells and became more pronounced in the late phase, during which time a marked decrease in the amount of sialidase-sensitive sialic acid present in the plasma membrane also took place. The erythrocytes of AH 7974F-bearing rats (which died from tumor growth much earlier than the AH 7974-bearing rats) had a similar, but less marked, decrease in their electrophoretic mobility and in the amount of sialidase-sensitive sialic acid present. These erythrocytes all had somewhat abnormal cell morphology, their shapes being slightly spherocytic.

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Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Cited by 3 publications

References 19 publications

Close Interrelationship of Sphingomyelinase and Caveolin in Triton X-100-lnsoluble Membrane Microdomains

Close Interrelationship of Sphingomyelinase and Caveolin in Triton X-100-lnsoluble Membrane Microdomains

Lipid Composition of the Plasma Membrane Isolated from Hyperplastic Nodules of Rat Liver1

Changes in the cell membrane of erythrocytes in tumor-bearing rats.

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