Phospholipids diffusion on the surface of model lipid droplets

Asfia, Shima; Seemann, Ralf; Fleury, Jean‐Baptiste

doi:10.1016/j.bbamem.2022.184074

Cited by 3 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This can be easily understood as DOPE lipids reduce the bilayer packing and increase the number of defects inside the bilayer, which facilitates the AuNPs translocation. 43,44 Furthermore, adding 10% DOPS (in total molar ratio)…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle Translocation Across a Droplet Interface Bilayer via Dark-Field Microscopy

Fleury

moro

Faure

et al. 2023

Preprint

View full text Add to dashboard Cite

For a wide range of biomedical applications, measuring the transport of metal nanoparticles across a cell membrane is of tremendous interest. Such studies are gaining high importance due to applications, especially in the field of drug delivery using NPs. In this work, we measure the passive transport of arginine-functionalized ultra-small gold nanoparticles (AuNPs) with sizes lower than 3 nm across a planar lipid bilayer formed with the droplet interface bilayer (DiB) technique. The AuNPs transport is monitored using dark-field microscopy, which enables the tracking of AuNPs without the use of fluorescent labeling. The measurements of high numbers of events provide a robust quantitative estimation of AuNP translocation dynamics and energy barrier obtained from the lipid bilayer translocation analysis. Interestingly, we observed that the arginine number coated onto AuNP and the bilayer packing control the AuNPs translocation accross the lipid bilayer.

show abstract

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle Translocation Across a Droplet Interface Bilayer via Dark-Field Microscopy

Fleury

moro

Faure

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The model LD surface was covered with a phospholipid monolayer at the buffer-triolein interface composed of DOPC:DOPE with a molar ratio of 3:1. As DOPE contains 2 w% of the fluorescent phospholipid DOPE-Cy5, we can perform FRAP experiments on this monolayer (see Figure 3) [27]. The diffusion coefficient extracted from the FRAP data is ≈8 µm 2 •s −1 when the total phospholipid concentration in triolein used to form the monolayer is equal or above 1 w%; see Figure 3 [34].…”

Section: Effect Of Adrp On the Phospholipid Diffusion Properties Of A...mentioning

confidence: 99%

“…During the following fluorescence recovery, image acquisition was continued for at least 5 min to be able to observe the full recovery. The thus obtained fluorescence recovery (FRAP) data were directly fitted with the Soumpasis equation using Python [26,27,34]. All experiments were performed at a room temperature of 23 • C.…”

Section: Confocal Imaging and Frap Experimentsmentioning

confidence: 99%

“…In this manuscript, we investigate the effects of ADRP on phospholipid diffusion and the 3D shape of lipid droplets embedded in a bilayer. For that, we used two different microfluidic setups: One for the fabrication of bilayer-embedded LDs and a second to mimic the surface of a single LD [26,27]. Using the first setup, we found that ADRP remains preferentially localized on the surfaces of bilayer-embedded LDs and investigated the impact of ADRP on the 3D geometry of bilayer-embedded LDs.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, we measured the effect of ADRP concentration on the exchange rate of phospholipid molecules between the monolayer and bilayer [26] by performing fluorescent recovery after photobleaching (FRAP) experiments. With the second setup, we determined the phospholipid diffusion on an artificial LD's surface as a function of ADRP concentration [27] by FRAP measurements.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bilayer-Embedded Lipid Droplets Coated with Perilipin-2 Display a Pancake Shape

Puza

Asfia

Seemann

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Lipid droplets (LD) are organelles localized in the membrane of the endoplasmic reticulum (ER) that play an important role in many biological functions. Free LDs that have been released from the ER membrane and are present in the cytosol resemble an oil-in-water emulsion. The surface of an LD is coated with a phospholipid monolayer, and the core of an LD is composed of neutral lipids. Adipose differentiation-related protein (ADRP), also known as perilipin-2, is a protein that surrounds the LD, together with the phospholipid monolayer. ADRP molecules are involved in assisting in the storage of neutral lipids within LDs. In this article, we focus our interest on the influence of ADRP molecules on the 3D shape of bilayer-embedded LDs and the diffusion of phospholipids in the monolayer covering LDs. For this study, we employed two different microfluidic setups: one to produce and explore bilayer-embedded LDs and a second one to mimic the surface of a single LD. Using the first setup, we demonstrate that ADRP molecules stay preferentially localized on the surfaces of bilayer-embedded LDs, and we study their 3D-shape in the presence of ADRP. Using the second setup, we performed FRAP experiments to measure the phospholipid diffusion on a model LD surface as a function of the ADRP concentration. Although the presence of proteins on the LD surface minimally affects the phospholipid and protein motility, ADRP appears to have a significant effect on the 3D structure of LDs embedded in the bilayer.

show abstract

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Mandal,

Brandt,

Tempra

et al. 2024

Preprint

View full text Add to dashboard Cite

Cellular membranes are composed of lipids typically organized in a double-leaflet structure. Interactions between these two leaflets - often referred to as interleaflet coupling - play a crucial role in various cellular processes. Despite extensive study, the mechanisms governing such interactions remain incompletely understood. Here, we investigate the effects of interleaflet coupling from a specific point of view, i.e. by comparing diffusive dynamics in bilayers and monolayers, focusing on potential lipid-specific interactions between opposing leaflets. Through quantitative fluorescence microscopy techniques, we characterize lipid diffusion and mean molecular area in monolayers and bilayers composed of different lipids. Our results suggest that the observed decrease in bilayer lipid diffusion compared to monolayers depends on lipid identity. Furthermore, our analysis suggests that lipid acyl chain structure and spatial configuration at the bilayer may strongly influence interleaflet interactions and dynamics in bilayers. These findings provide insights into the role of lipid structure in mediating interleaflet coupling and underscore the need for further experimental investigations to elucidate the underlying mechanisms.

show abstract

Phospholipids diffusion on the surface of model lipid droplets

Cited by 3 publications

References 46 publications

Gold Nanoparticle Translocation Across a Droplet Interface Bilayer via Dark-Field Microscopy

Gold Nanoparticle Translocation Across a Droplet Interface Bilayer via Dark-Field Microscopy

Bilayer-Embedded Lipid Droplets Coated with Perilipin-2 Display a Pancake Shape

A comparison of lipid diffusive dynamics in monolayers and bilayers in the context of interleaflet coupling

Contact Info

Product

Resources

About