Phospholipids and Related Substances as Growth Substrates for Soil Microorganisms

Schatz, Albert; Adelson, Lionel M.; Trelawny, Gilbert S.

doi:10.1128/am.4.5.223-228.1956

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…The addition of lecithin to cultivations of different actinomycetes isolated from soil was beneficial in previous studies regarding product formation (Adelson et al, 1957; Brock, 1956; Choi & Cho, 2004; Schatz et al, 1956). Due to the positive effects for similar microorganisms as L. aerocolonigenes lecithin supplemented cultivations were performed.…”

Section: Resultsmentioning

confidence: 99%

Macroparticle‐enhanced cultivation of Lentzea aerocolonigenes: Variation of mechanical stress and combination with lecithin supplementation for a significantly increased rebeccamycin production

Schrinner

Schrader

Niebusch

et al. 2021

Biotech & Bioengineering

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The actinomycete Lentzea aerocolonigenes produces the antitumor antibiotic rebeccamycin. In previous studies the rebeccamycin production was significantly increased by the addition of glass beads during cultivation in different diameters between 0.5 and 2 mm and the induced mechanical stress by the glass beads was proposed to be responsible for the increased production. Thus, this study was conducted to be a systematic investigation of different parameters for macroparticle addition, such as bead diameter, concentration, and density (glass and ceramic) as well as shaking frequency, for a better understanding of the particle‐induced stress on L. aerocolonigenes. The induced stress for optimal rebeccamycin production can be estimated by a combination of stress energy and stress frequency. In addition, the macroparticle‐enhanced cultivation of L. aerocolonigenes was combined with soy lecithin addition to further increase the rebeccamycin concentration. With 100 g L−1 glass beads in a diameter of 969 µm and 5 g L−1 soy lecithin a concentration of 388 mg L−1 rebeccamycin was reached after 10 days of cultivation, which corresponds to the highest rebeccamycin concentrations achieved in shake flask cultivations of L. aerocolonigenes stated in literature so far.

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Section: Resultsmentioning

confidence: 99%

Macroparticle‐enhanced cultivation of Lentzea aerocolonigenes: Variation of mechanical stress and combination with lecithin supplementation for a significantly increased rebeccamycin production

Schrinner

Schrader

Niebusch

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…The results of these investigations are of general interest with respect to the metabolism of actinomycetes, a group of organisms that has 1957] been less extensively studied firom this point of view than other microflora (Schatz et al, 1956a;Schatz et al, 1955). As regaids demyelination, the ability of this actinomycete to catabolize specific components of myelin in vitro suggests enzymes, reactions, and loci that may be involved in the degradation of intact myelin in spinal cord tissue (Adelson et al, 1956).…”

Section: Discussionmentioning

confidence: 99%

“…Actinomycete 58-B, a culture originally isolated from sheep manure (Schatz et al, 1956a), resembled the two original strains of Streptomyces griseus that produced streptomycin (Schatz et al, 1944). For respirometric studies, actinomyeete 58-B was grown in agitated cultures of Krebs-Ringer-phosphate solution (Umbreit et al, 1951) supplemented with 0.0005 per cent FeCl3 6H20, 0.05 per cent (NH4)2SO4, 0.2 per cent Armour B.S.C.…”

Section: Methodsmentioning

confidence: 99%

“…The yeast extract (Difco), stearic and palmitic acids, and lipid substrates were supplied in suspension at the rate of 100.0 mg per vessel. The water-insoluble materials were emulsified in the buffered synthetic mineral solution by grinding with a Teflon tissue homogenizer, a procedure found satisfactory for previous studies with these substances (Schatz et al, 1956a). The animal cephalin (Nutritional Biochemicals Corp.) was a mixture of phosphatidyl serine, phosphatidyl ethanolamine, and diphosphoinositide (Folch, 1949).…”

Section: Methodsmentioning

confidence: 99%

“…These considerations motivated earlier studies in which 132 soil microflora were surveyed for their ability to develop on phospholipids ancf related substances (Schatz et al, 1956a). Of the 109 actinomycetes included in the 132 organisms, 6 proved capable of demyelinating fresh blocks of bovine spinal cord in vitro (Adelson et al, 1956).…”

mentioning

confidence: 99%

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