Phospholipid synthesis in the preimplantation mouse embryo

Pratt, Hester P. M.

doi:10.1530/jrf.0.0580237

Cited by 53 publications

(37 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether preimplantation embryos also contain an acetyl transferase capable of con¬ verting the lyso-phosphocholines into active PAF-acether is still to be determined. Preimplantation embryos, prelabelled with [3H]choline, lost 10-40% of labelled phospholipids during a 24-h culture period and it was suggested that this was due to turnover of membrane phospholipids (Pratt, 1980). In view of the present findings, it will be necessary to perform pulse-chase studies to determine whether some of this label is released as embryo-derived PAF. This work was supported by a Grant from the Australian Research Grants Scheme (ARGS).…”

Section: Methodsmentioning

confidence: 72%

See 1 more Smart Citation

Partial characterization of the embryo-derived platelet-activating factor in mice

O’Neill¹

1985

Reproduction

147

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 72%

“…The preimplantation mouse embryo was capable of incorporating methyl-[3H]choline into phosphocholine, phosphatidylcholine and lysolecithins, via the Kennedy pathway (Pratt, 1980). Embryo extracts showed considerable phospholipase A activity, resulting in the deacylation of exogenous phospholipids (Pratt, 1980).…”

Section: Methodsmentioning

confidence: 99%

Partial characterization of the embryo-derived platelet-activating factor in mice

O’Neill¹

1985

Reproduction

147

View full text Add to dashboard Cite

show abstract

“…Cell-cell compaction, which precedes normal blastulation, depends on the lipid composition of embryo plasma membranes (Pratt, 1978) and, as shown in sheep (Walker et al, 1998), can be compromised in conditions that alter embryo lipid content artificially.…”

Section: Discussionmentioning

confidence: 99%

Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida

McEvoy

Coull²,

Broadbent³

et al. 2000

Reproduction

260

212

View full text Add to dashboard Cite

Cattle, pig and sheep oocytes isolated from healthy cumulus-oocyte complexes were pooled, within species, to provide samples of immature denuded oocytes with intact zona pellucida (n = 1000 per sample) for determination of fatty acid mass and composition in total lipid, constituent phospholipid and triglyceride. Acyl-containing lipid extracts, transmethylated in the presence of a reference penta-decaenoic acid (15:0), yielded fatty acid methyl esters which were analysed by gas chromatography. Mean (± SEM) fatty acid content in samples of pig oocytes (161 ± 18 µg per 1000 oocytes) was greater than that in cattle (63 ± 6 µg; P < 0.01) and sheep oocytes (89 ± 7 µg; P < 0.05). Of 24 fatty acids detected, palmitic (16:0; 25-35%, w/w), stearic (18:0; 14-16%) and oleic (18:1n-9; 22-26%) acids were most prominent in all three species. Saturated fatty acids (mean = 45-55%, w/w) were more abundant than mono-(27-34%) or polyunsaturates (11-21%). Fatty acids of the n-6 series, notably linoleic (18:2n-6; 5-8%, w/w) and arachidonic acid (20:4n-6; 1-3%), were the most abundant polyunsaturates. Phospholipid consistently accounted for a quarter of all fatty acids in the three species, but ruminant oocytes had a lower complement of polyunsaturates (14-19%, w/w) in this fraction than pig oocytes (34%, w/w) which, for example, had a three-to fourfold greater linoleic acid content. An estimated 74 ng of fatty acid was sequestered in the triglyceride fraction of individual pig oocytes compared with 23-25 ng in ruminant oocytes (P < 0.01). It is concluded that the greater fatty acid content of pig oocytes is primarily due to more abundant triglyceride reserves. Furthermore, this speciesspecific difference, and that in respect of polyunsaturated fatty acid reserves, may underlie the contrasting chilling, culture and cryopreservation sensitivities of embryos derived from pig and ruminant (cattle, sheep) oocytes.

show abstract

“…A 13-fold increase in phosphocholine (PCho) synthesis in mouse embryos from the two-to eight-cell stages has been previously reported by supplementing the culture medium with labeled choline (methyl-3H-choline) as a lipid precursor, suggesting not only membrane synthesis activity but also membrane remodeling during preimplantation development [16]. The enzyme cytidine 5′-triphosphate phosphocholine cytidylyltransferase, which participates in the biosynthesis of choline-containing PL, has been shown to be present in mouse oocyte and early embryo extracts using a specific enzymatic assay [17].…”

Section: Introductionmentioning

confidence: 90%

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

et al. 2012

View full text Add to dashboard Cite

Knowledge of the lipids present in individual preimplantation embryos is of interest in fundamental studies of embryology, in attempts to understand cellular pluripotency and in optimization of in vitro culture conditions necessary for the application and development of biotechnologies such as in vitro fertilization and transgenesis. In this work, the profiles of fatty acids and phospholipids (PL) in individual mouse preimplantation embryos and oocytes were acquired using an analytical strategy based on desorption electrospray ionization mass spectrometry (DESI-MS). The methodology avoids sample preparation and provides information on the lipids present in these microscopic structures. Differences in the lipid profiles observed for unfertilized oocytes, two-and four-cell embryos, and blastocysts were characterized. For a representative set of embryos (N= 114) using multivariate analysis (specifically principal component analysis) unfertilized oocytes showed a narrower range of PL species than did blastocysts. Two-and four-cell embryos showed a wide range of PLs compared with unfertilized oocytes and high abundances of fatty acids, indicating pronounced synthetic activity. The data suggest that the lipid changes observed in mouse preimplantation development reflect acquisition of a degree of cellular membrane functional and structural specialization by the blastocyst stage. It is also noteworthy that embryos cultured in vitro from the two-cell through the blastocyst stage have a more homogeneous lipid profile as compared with their in vivo-derived counterparts, which is ascribed to the restricted diversity of nutrients present in synthetic culture media. The DESI-MS data are interpreted from lipid biochemistry and previous reports on gene expression of diverse lipids known to be vital to early embryonic development.

show abstract

Phospholipid synthesis in the preimplantation mouse embryo

Cited by 53 publications

References 34 publications

Partial characterization of the embryo-derived platelet-activating factor in mice

Partial characterization of the embryo-derived platelet-activating factor in mice

Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

Contact Info

Product

Resources

About