Phospholipid requirements of ATPase of Escherichia coli

Peter, Horst W.; Ahlers, J.

doi:10.1016/0003-9861(75)90108-3

Cited by 24 publications

(5 citation statements)

References 14 publications

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“…The results generated here are generally in accord with prior lipid analyses in E. coli, which show PE lipids to be the most abundant (∼70 to 90% of total phospholipids), PG to be the next most abundant (∼5 to 15%), and a more minor contribution of cardiolipin (∼1 to 9 %). − Varying reports additionally record phosphatidic acid (PA), phosphatidylserine (PS), or lysophosphatidylethanolamine (LPE) lipids. − However, these latter classes seem to be more minor components, with PA being an intermediate in phospholipid synthesis and PS being an intermediate for PE lipids. Fatty acids identified previously range from 10:0 to 19:1, but 16:0, 18:1, and 16:1 were the most common. ,− …”

Section: Resultssupporting

confidence: 85%

Characterization of the In Vivo Deuteration of Native Phospholipids by Mass Spectrometry Yields Guidelines for Their Regiospecific Customization

Keller,

Zhang,

Qian

et al. 2023

Anal. Chem.

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Customization of deuterated biomolecules is vital for many advanced biological experiments including neutron scattering. However, because it is challenging to control the proportion and regiospecificity of deuterium incorporation in live systems, often only two or three synthetic lipids are mixed together to form simplistic model membranes. This limits the applicability and biological accuracy of the results generated with these synthetic membranes. Despite some limited prior examination of deuterating Escherichia coli lipids in vivo, this approach has not been widely implemented. Here, an extensive mass spectrometrybased profiling of E. coli phospholipid deuteration states with several different growth media was performed, and a computational method to describe deuterium distributions with a onenumber summary is introduced. The deuteration states of 36 lipid species were quantitatively profiled in 15 different growth conditions, and tandem mass spectrometry was used to reveal deuterium localization. Regressions were employed to enable the prediction of lipid deuteration for untested conditions. Small-angle neutron scattering was performed on select deuterated lipid samples, which validated the deuteration states calculated from the mass spectral data. Based on these experiments, guidelines for the design of specifically deuterated phospholipids are described. This unlocks even greater capabilities from neutron-based techniques, enabling experiments that were formerly impossible.

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Section: Resultssupporting

confidence: 85%

Characterization of the In Vivo Deuteration of Native Phospholipids by Mass Spectrometry Yields Guidelines for Their Regiospecific Customization

Keller,

Zhang,

Qian

et al. 2023

Anal. Chem.

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“…This reduction in alginic acid polysac-charide production during phosphate-limited growth may be a consequence of alterations in membrane phospholipid content. Many enzymes are dependent on or influenced by the presence of phospholipids (35) such as the C5Sisoprenoid alcohol kinase (15,36), ATPase (11,32), and reduced adenine dinucleotide oxidase (6,8). Therefore, the reduction in polysaccharide synthesis may be a reflection of the importance of specific phospholipids in maintaining optimum activity of various membrane-bound enzymes.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylcholine stimulates capsule formation of phosphate-limited mucoid Pseudomonas aeruginosa

1988

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Production of both alginic acid and lipopolysaccharide by a mucoid strain of Pseudomonas aeruginosa, SRM-3, was studied in a chemostat system during growth under nutrient-limiting conditions chosen to reflect the chronic growth conditions in the lungs of cystic fibrosis patients. Since mucoid strains have been shown to elaborate extracellular proteases and phospholipase C, nitrogen and phosphate limitation were selected for analysis. A modified alginate-promoting medium containing either 1 mM glutamate or 0.05 mM K2HPO4 as limiting nutrient and doubling times of 1.6 to 15.7 h were used. Under nitrogen limitation, strain SRM-3 produced 1.4 mg of uronic acid per mg (dry weight) of cells at all doubling times studied. However, phosphate limitation resulted in the synthesis of only 0.4 mg of uronic acid per mg (dry weight) of cells. The role of phosphate in alginic acid polysaccharide production was further investigated by using phosphorylcholine, a product of phospholipase C activity on phosphatidylcholine, the major lung surfactant. Not only were mucoid cells capable of utilizing phosphorylcholine for growth, but a highly specific interaction occurred among phosphorylcholine, alginate, and whole cells, resulting in greatly enhanced culture viscosity. Electron micrographs showed the gradual formation of a capsule during growth on phosphorylcholine, indicating that the mucoid strain has the ability to utilize surfactant not only as a nutrient source but also for constructing a capsule with greatly enhanced adhesive properties.

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“…Hare (158) reported generally similar results using a partially purified, deoxycholatesolubilized ATPase complex from the same organism and attributed the stimulatory and sensitizing properties of the added phospholipids (of which phosphatidyl choline, phosphatidyl serine, and phosphatidyl ethanolamine were the most potent) to their abilities to replace those that were lost during the solubilization procedures. It is interesting to note that phospholipids are also required for maximal ATPase activity in mitochondrial and other bacterial F0-F, preparations (210,380,425) and possibly also in purified F1 (315).…”

Section: Purification and Properties Of The F-f Complexmentioning

confidence: 99%