Phospholipid requirement of microsomal chitinase fromMucor mucedo

Edifenphos (Hinosan) reduces hyphal extension, hyphal growth unit length and phosphatidylcholine content of Fusarium graminearum A3/5, but has no effect on specific growth rate

“…The interaction of membrane proteins with membrane lipids is well documented (Lee, 1987;Lenaz, 1987) and some fungal enzymes have specific phospholipid requirements for optimal activity (Duran & Cabib, 1978;Vermeulen & Wessels, 1983;Montgomery & Gooday, 1985;Humphreys & Gooday, 1984). Robson et al (1989) suggested that if the activity (K,,,, V ) of enzymes involved in tip extension is diminished when the phospholipid environment is altered, then the mean hyphal extension rate will be reduced.…”

Lipids in the Structure and Function of Fungal Membranes

“…Apart from a handful of records, phosphatidyl glycerol appears to be confined to the order Peronosporales of the Oomycetes. Phospholipid requirements have been demonstrated for chitin synthase activity in Schizophyllum commune and Coprinus cinereus (Vermeulen and Wessels 1983;Montgomery and Gooday 1985; see also Chapters 5 and 6) and for chitinase in Mucor mucedo (Humphreys and Gooday 1984; see also Chapter 5). Strong evidence has recently been provided for the role of phosphatidyl inositol in glucomannan synthesis by Rhizoctonia cerealis (Robson 1987).…”

Evidence for the involvement of acylglycerides on chitin synthetase activity in Candida albicans

“…In Candida albicans the enzyme is located, partly active, in the plasma membrane [3,4] as well as in cytoplasmic particles containing the zymogenic enzyme (chitosomes) [4,5], similar to the enzyme location in Saccharomyces cerevisiae [6,7]. The activity of the membrane-bound enzymes may be modulated by changes in the lipid environment [9][10][11][12][13]. The activity of the membrane-bound enzymes may be modulated by changes in the lipid environment [9][10][11][12][13].…”